Engineering UDP-Glycosyltransferase UGTPg29 for the Efficient Synthesis of Ginsenoside Rg3 from Protopanaxadiol.

Rare ginsenosides Rg3 and Rh2, which exhibit diverse pharmacological effects, are derivatives of protopanaxadiol (PPD). UDP-glycosyltransferases, such as the M315F variant of Bs-YjiC (Bs-YjiCm) from Bacillus subtilis and UGTPg29 from Panax ginseng, can efficiently convert PPD into Rh2 and Rh2 into Rg3, respectively. In the present study, the N178I mutation of Bs-YjiCm was introduced, resulting in an increase in Rh2 production.

Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three Species Grown in Special High-Cold Environment of Northwestern Yunnan, China.

is a medicinal plant with obvious rhizosphere effects. At present, there are many plants with high application value but low recognition and resource reserves in the northwestern area of Yunnan province, China. In this study, metagenomics was used to analyze the microbial diversity and community structure of rhizosphere soil of , , and plants grown in a special high-cold environment of northwestern Yunnan, China, at different altitudes ranging from 3225 to 4353 m.

Identification of sucrose synthase from to favor biocatalytic glycosylation.

Sucrose synthase (SuSy, EC 2.4.1.

New insights into the reverse of chromium-induced reprotoxicity of pregnant mice by melatonin.

Hexavalent chromium Cr(VI) is a well-known environmental toxic metal that causes reprotoxicity in pregnant females. There are currently no appropriate interventions or treatments for Cr(VI) exposure during pregnancy. Herein, the protective effect of melatonin (MLT) against Cr(VI)-induced reprotoxicity is investigated by administrating MLT to pregnant mice exposed to Cr(VI).

Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy.

Cancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer.

Mode discrimination in dissipatively coupled laser arrays.

We show that dissipative coupling between an array of passive optical resonators creates a ladder of decay rates in the complex eigenfrequencies. This effect promotes mode discrimination in laser arrays, while the lowest-order and highest-order modes exhibit the highest and lowest lasing thresholds, respectively. The array supermodes and their corresponding eigenfrequencies are calculated analytically through a tight-binding model, and the single-mode operation range is derived.

A Case Study of Dynamic Response Analysis and Safety Assessment for a Suspended Monorail System.

A suspended monorail transit system is a category of urban rail transit, which is effective in alleviating traffic pressure and injury prevention. Meanwhile, with the advantages of low cost and short construction time, suspended monorail transit systems show vast potential for future development. However, the suspended monorail has not been systematically studied in China, and there is a lack of relevant knowledge and analytical methods.