High-Level Production of Nicotinamide Mononucleotide by Engineered .

Nicotinamide mononucleotide (NMN), a key precursor of NAD, is a promising nutraceutical due to its excellent efficacy in alleviating aging and disease. The bioproduction of NMN faces challenges related to incomplete metabolic engineering and insufficient metabolic flux. Here, we constructed an NMN synthesis pathway in BW25113 by deleting the competitive pathway genes and introducing three heterologous genes encoding the key enzymes nicotinamide phosphoribosyltransferase (NAMPT), phosphoribosyl pyrophosphate synthetase and an NMN transporter.

Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli.

Background: Melatonin has attracted substantial attention because of its excellent prospects for both medical applications and crop improvement. The microbial production of melatonin is a safer and more promising alternative to chemical synthesis approaches. Researchers have failed to produce high yields of melatonin in common heterologous hosts due to either the insolubility or low enzyme activity of proteins encoded by gene clusters related to melatonin biosynthesis.

Transcriptomic profiles of Dunaliella salina in response to hypersaline stress.

Background: Dunaliella salina is a good model organism for studying salt stress. In order to have a global understanding of the expression profiles of Dunaliella salina in response to hypersaline stress, we performed quantitative transcriptomic analysis of Dunaliella salina under hypersaline stress (2.5 M NaCl) of different time duration by the second and third generation sequencing method.