Publications by authors named "Jiahui Ong"
Human parvovirus B19 virus-like particle formation in Nicotiana benthamiana.
Protein Expr Purif
February 2025
Article Synopsis
- There is growing interest in using plants to produce vaccine components, and this study shows successful production of the human parvovirus B19 (B19V) capsid protein (VP2) in Nicotiana benthamiana cells.
- By cloning the VP1 and VP2 genes and using estrogen-inducible promoters, the researchers were able to significantly enhance VP2 expression, especially with the help of codon optimization that improved production over 30-fold.
- The expressed VP2 proteins formed spherical particles (virus-like particles) and were primarily found in the cytoplasm, indicating a viable plant-based method for producing B19V VLPs.