Lack of dominant-negative activity for tumor-related ZNRF3 missense mutations at endogenous levels.

ZNRF3, a negative regulator of β-catenin signaling, removes Wnt receptors from the membrane. Currently, it is unknown which tumor-associated variants can be considered driver mutations and through which mechanisms they contribute to cancer. Here we show that all truncating mutations analyzed at endogenous levels exhibit loss-of-function, with longer variants retaining partial activity.

Emulsifying and ethanol-induced gelling properties of capsicum pectin: The impact of capsicum variety and deproteinization using the Sevag method.

This study investigated the emulsifying and ethanol-induced gelling properties of capsicum pectins from bell pepper and chili pepper, and also examined the effect of deproteinization (using the Sevag method). The research revealed that bell pepper pectin (BPP) formed smaller aggregates (337 nm at pH 2) with a higher hydrophobicity in solution due to its lower molecular weight (2.02 × 10 g/mol) and higher degree of esterification (82.

RNF43 and ZNRF3: Versatile regulators at the membrane and their role in cancer.

RNF43 and ZNRF3 are recognized as important regulators of Wnt/β-catenin signaling by maintaining Wnt-receptors at minimal essential levels. In various cancer types, particularly gastrointestinal tumors, mutations in these genes lead to abnormal Wnt-dependent activation of β-catenin signaling. However, recent findings implicate RNF43/ZNRF3 also in the regulation of other tumor-related proteins, including EGFR, BRAF, and the BMP-signaling pathway, which may have important implications for tumor biology.

Assessment of the Psychometric Properties of the Perceived Empathic and Social Self-Efficacy Scale in Chinese Adolescents.

Purpose: There is a burgeoning interest in nurturing adolescents' social and emotional skills, acknowledging the significant influence these abilities have on adolescents' social development and overall well-being. The Perceived Empathic and Social Self-Efficacy Scale (PESSE) emerges as a promising tool designed to capture adolescents' self-perceptions regarding their self-efficacy in empathic and social domains. This study seeks to investigate the psychometric properties of the Chinese Version of the Perceived Empathic and Social Self-Efficacy Scale (PESSE) by examining its factor structure, measurement invariance across gender and age groups, along with its predictive validity concerning adolescent subjective and social well-being.

Starch chain-length distributions affect the processing and digestion characteristics of proso millet starch.

Starch chain-length distributions play a key role in regulating the processing and digestion characteristics of proso millet starch. Waxy proso millet starch has higher endothermic enthalpy (13.06-16.

Unraveling the impact of AXIN1 mutations on HCC development: Insights from CRISPR/Cas9 repaired AXIN1-mutant liver cancer cell lines.

Background: Hepatocellular carcinoma (HCC) is a highly aggressive liver cancer with significant morbidity and mortality rates. AXIN1 is one of the top-mutated genes in HCC, but the mechanism by which AXIN1 mutations contribute to HCC development remains unclear.

Methods: In this study, we utilized CRISPR/Cas9 genome editing to repair AXIN1-truncated mutations in five HCC cell lines.

Amylopectin chain length distributions and amylose content are determinants of viscoelasticity and digestibility differences in mung bean starch and proso millet starch.

This study aimed to reveal the underlying mechanisms of the differences in viscoelasticity and digestibility between mung bean starch (MBS) and proso millet starch (PMS) from the viewpoint of starch fine molecular structure. The contents of amylopectin B2 chains (14.94-15.

The Difficulties in Interpersonal Regulation of Emotions Scale (DIRE): Factor Structure and Measurement Invariance across Gender and Two Chinese Youth Samples.

Effective interpersonal emotion regulation (IER) strategies have been found to be meaningful predictors for positive psychological functioning. The Difficulties in Interpersonal Regulation of Emotions Scale (DIRE) is a measure developed to assess maladaptive IER strategies. This study aimed to examine the psychometric properties of the Chinese version of DIRE using two college student samples (Sample 1: = 296; Sample 2: = 419).

A bifactor representation of the Center for Epidemiological Studies Depression Scale for children: gender and age invariance and implications for adolescents' social and academic adjustment.

Background: The Center for Epidemiological Studies Depression Scale for Children (CES-DC) is a widely used scale for screening depressive symptoms in children and adolescents. This study aims to uncover the optimal factor structure of the DES-DC and presents an alternate conceptualization of adolescent depression by estimating bifactor models and several competing models using a sample of Chinese adolescents.

Methods: The participants were 533 adolescents (49.

Application of bacteriophage φPaP11-13 attenuates rat infection lesions by promoting keratinocytes apoptosis via inhibiting PI3K/Akt pathway.

Acne vulgaris caused by antibiotic-resistant () infection is difficult to treat conventionally. Phages have been suggested as a potential solution, but research on the mechanism of phage treatment is inadequate. This research investigates the underlying molecular mechanisms of phage φPaP11-13 attenuating -induced inflammation in rat models.

Development of multiplex allele-specific RT-qPCR assays for differentiation of SARS-CoV-2 Omicron subvariants.

Quick differentiation of current circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmissions. However, the widely applied gene sequencing method is time-consuming and costly especially when facing recombinant variants, because a large part or whole genome sequencing is required. Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a quick and cost-effective method for SNP (single nucleotide polymorphism) genotyping and has been successfully applied for SARS-CoV-2 variant screening.

Other-Oriented Empathetic Concern as the Underlying Factor of Extrinsic Emotion Regulation Motives and Its Positive Application for Social Well-Being.

Purpose: In recent years, research on interpersonal emotion regulation has garnered increasing attention. Extrinsic emotion regulation (EER) or the regulation of others' emotions is associated with individual personal relationships and mental health outcomes. However, investigations into why people engage in extrinsic emotion regulation are relatively rare.

Orexin recruits non-selective cationic conductance and endocannabinoid to dynamically modulate firing of caudal pontine reticular nuclear neurones.

The neuropeptide orexin is involved in motor circuit function. However, its modulation on neuronal activities of motor structures, integrating orexin's diverse downstream molecular cascades, remains elusive. By combining whole-cell patch-clamp recordings and neuropharmacological methods, we revealed that both non-selective cationic conductance (NSCC) and endocannabinoids (eCBs) are recruited by orexin signalling on reticulospinal neurones in the caudal pontine reticular nucleus (PnC).

Psychometric Properties of Interpersonal Regulation Questionnaire for Chinese College Students: Gender Differences and Implications for Well-Being.

Intrapersonal emotion dysregulation has been found to be a transdiagnostic predictor in the development of almost all affective disorders. Interpersonal resources are also involved in achieving people's emotion regulation goals. The Interpersonal Regulation Questionnaire (IRQ) has been developed to assess the tendency and efficacy of people using external resources to help manage their emotions.

Heightened Tameness and Accelerated Handling-Habituation in 3×Tg-AD Mice on a B6;129 Genetic Background.

Background: The triple transgenic mouse model of Alzheimer's disease (3×Tg-AD) has gained popularity in Alzheimer's research owing to the progressive development of both amyloid- and tau pathologies in its brain. Prior handling-habituation, a necessary preparation procedure that reduces anxiety and stress in rodents, was seldom described in the literature involving these mice and needs to be addressed.

Objective: We sought to determine whether 3×Tg-AD mice differ from B6;129 genetic control mice in terms of tameness and prior habituation to handling.

Rats Display Sexual Dimorphism in Phosphorylation of Brain Tau with Age.

Background: Women have a two-fold higher risk than men to Alzheimer's disease (AD) at midlife. Larger brain tau burden was consistently shown in older women than age-matched men. The biological basis for this gender disparity remains elusive.

Quality of pediatric clinical practice guidelines.

Background: There is a lack of a comprehensive evaluation for pediatric clinical practice guidelines (CPGs) published in recent years. Here, we assessed the quality of pediatric CPGs, considering factors that might affect their quality. The aim of the study is to promote a more coherent development and application of CPGs.

sEMG-Based Neural Network Prediction Model Selection of Gesture Fatigue and Dataset Optimization.

The fatigue energy consumption of independent gestures can be obtained by calculating the power spectrum of surface electromyography (sEMG) signals. The existing research studies focus on the fatigue of independent gestures, while the research studies on integrated gestures are few. However, the actual gesture operation mode is usually integrated by multiple independent gestures, so the fatigue degree of integrated gestures can be predicted by training neural network of independent gestures.

Antifungal resistance-modifying multiplexing action of Momordica charantia protein and phosphorylated derivatives on the basis of growth-dependent gene coregulation in Candida albicans.

Unlabelled: Fungal growth-dependent gene coregulation is strongly implicated in alteration of gene-encoding target proteases ruling with an antifungal resistance niche and biology of resistant mutants. On the basis of multi-alterative processes in this platform, the resistance-modifying strategy is designed in ketoconazole resistant Candida albicans and evaluated with less selective Momordica charantia protein and allosterically phosphorylated derivatives at the Thr102, Thr24 and Thr255 sites, respectively. We demonstrate absolutely chemo-sensitizing efficacy regarding stepwise-modifying resistance in sensitivity, by a load of only 26.

Dietary supplementation with polysaccharides from Ziziphus Jujuba cv. Pozao intervenes in immune response via regulating peripheral immunity and intestinal barrier function in cyclophosphamide-induced mice.

Ziziphus Jujuba cv. Pozao has been consumed as a traditional fruit with regional characteristics in China for a long time; however, fewer studies on polysaccharides from Ziziphus Jujuba cv. Pozao (JP) have been documented.

Effects of zinc oxide nanoparticles on sludge anaerobic fermentation: phenomenon and mechanism.

Zinc oxide nanoparticles (ZnO NPs) production and usage might lead to a large discharge of ZnO NPs into the natural environment, raising concerns of pollution and ecological security. The effects of ZnO NPs on waste activated sludge hydrolytic acidification and microbial communities were studied in semi-continuous fermentation systems. The fermentation performance of eight ZnO NPs concentrations including ZnO NPs normal [0.

Correction to: The role of C-afferents in mediating neurogenic vasodilatation in plantar skin after acute sciatic nerve injury in rats.

An amendment to this paper has been published and can be accessed via the original article.

The role of C-afferents in mediating neurogenic vasodilatation in plantar skin after acute sciatic nerve injury in rats.

Background: Vasomotor regulation of dermal blood vessels, which are critical in the function of the skin in thermoregulatory control, involves both neural and non-neural mechanisms. Whereas the role of sympathetic nerves in regulating vasomotor activities is comprehensively studied and well recognized, that of sensory nerves is underappreciated. Studies in rodents have shown that severance of the sciatic nerve leads to vasodilatation in the foot, but whether sympathetic or sensory nerve fibers or both are responsible for the neurogenic vasodilatation remains unknown.

Response of extracellular polymeric substances and enzymatic activity to salinity for the waste activated sludge anaerobic fermentation process.

Salinity (NaCl) was used in waste activated sludge (WAS) anaerobic fermentation system which had been presented to greatly enhance the extracellular polymeric substance (EPS) production including protein and polysaccharide and short-chain fatty acids (SCFAs). Salinity enhanced soluble protein and polysaccharide (SB-EPS) release which was 4.04 times (protein) and 1.