Effect of mutation at c.493T>C locus of transcription factor HNF1α gene on its protein level.

Hepatocyte nuclear factor 1α (HNF1α) is a transcription factor that is crucial for the regulation to maintain the function of pancreatic β-cell, hepatic lipid metabolism, and other processes. Mature-onset diabetes of the young type 3 is a monogenic form of diabetes caused by HNF1α mutations. Although several mutation sites have been reported, the specific mechanisms remain unclear, such hot-spot mutation as the P291fsinsC mutation and the P112L mutation and so on.

Structural insight into SSE15206 in complex with tubulin provides a rational design for pyrazolinethioamides as tubulin polymerization inhibitors.

Tubulin protein is a promising target for antitumor drugs. Some tubulin inhibitors targeting the colchicine binding site are not substrates of the multidrug-resistance efflux pump, which can overcome the mechanism of drug resistance mediated by P-glycoprotein. SSE15206 is a colchicine binding site inhibitor with antiproliferative activity against different drug-resistant cell lines.

Structural insights into targeting of the colchicine binding site by ELR510444 and parbendazole to achieve rational drug design.

Microtubules consisting of α- and β-tubulin heterodimers have proven to be an efficient drug target for cancer therapy. A broad range of agents, including ELR510444 and parbendazole, can bind to tubulin and interfere with microtubule assembly. ELR510444 and parbendazole are colchicine binding site inhibitors with antiproliferative activities.

Erratum: Chemopreventive effect of 4'-hydroxychalcone on intestinal tumorigenesis in Apc mice.

[This corrects the article DOI: 10.3892/ol.2021.

Chemopreventive effect of 4'-hydroxychalcone on intestinal tumorigenesis in mice.

Chalcones and its derivatives are reported to exhibit anti-cancer effects in several cancer cell lines, including colon cancer cells. However, the anticancer effects and associated mechanisms of chalcones against intestinal tumorigenesis currently remain unclear. The aim of the present study was to investigate the chemopreventive effect of a chalcone derivative, 4'-hydroxychalcone (4-HC), in a transgenic adenomatous polyposis coli multiple intestinal neoplasia mouse model () of spontaneous intestinal adenomas.

The investigation on the role of mitochondrial fusion protein 1 in the development of myopia.

Purpose: The aim of this study is to preliminarily investigate the expression of mitochondrial fusion protein 1 (MFN1) in a lens-induced animal myopia (LIM) model and to explore the relationship between MFN1 and the visual development.

Materials And Methods: MFN1 gene expression in guinea pigs was examined during the development of minus LIM, 15 tri-colored guinea pigs were obtained, and one eye of each pig was randomly selected and treated with -7.00D lenses.

Correlation between polymorphisms in the MFN1 gene and myopia in Chinese population.

Aim: To explore whether genetic variations in the MFN1 gene are associated with low to moderate myopia in Chinese population.

Methods: The case-control association analysis was used. The study included 100 independent myopia patients (-0.

Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine.

A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected.

Potentiometric aptasensing of Listeria monocytogenes using protamine as an indicator.

Exposure to pathogens in recreational or drinking water is a serious public health concern. It is important to rapidly determine and identify trace levels of pathogens in real environmental samples. We report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of Listeria monocytogenes (LM), a pathogen widely distributed in the environment.

[Cytogenetics and application in domesticated silkworm Bombyx mori].

Chromosome identification and karyotype analyses of Bombyx mori (Lepidoptera) have long been hampered by the high number and the absence of suitable markers, such as centromeric position and chromosome bands. Recently, the cytological map has been constructed using comparative genomic hybridization-genomic in situ hybridization and BAC-based fluorescence in situ hybridization. Dense cytogenetic maps are being constructed by integrating cytological map with molecular linkage maps.