Generation of Zebrafish Maternal Mutants via Oocyte-Specific Knockout System.

Maternal mRNAs and proteins are produced during oogenesis by more than 60% of zebrafish genes. They are indispensable for fertilization and early embryogenesis. Generation and analysis of the maternal mutant is the most direct way to characterize the maternal function of the specific gene.

Identification of PANoptosis-related subtypes, construction of a prognosis signature, and tumor microenvironment landscape of hepatocellular carcinoma using bioinformatic analysis and experimental verification.

Background: Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide. PANoptosis is a recently unveiled programmed cell death pathway, Nonetheless, the precise implications of PANoptosis within the context of HCC remain incompletely elucidated.

Methods: We conducted a comprehensive bioinformatics analysis to evaluate both the expression and mutation patterns of PANoptosis-related genes (PRGs).

Asymmetry-enhanced attention network for Alzheimer's diagnosis with structural Magnetic Resonance Imaging.

Background And Objective: With the aging of the global population becoming severe, Alzheimer's disease (AD) has become one of the world's most common senile diseases. Many studies have suggested that the brain's left-right asymmetry is one of the possible diagnostic landmarks for AD. However, most published approaches to classification problems may not adequately explore the asymmetry between the left and right hemispheres.

EA-Net: Edge-aware network for brain structure segmentation via decoupled high and low frequency features.

Automatic brain structure segmentation in Magnetic Resonance Image (MRI) plays an important role in the diagnosis of various neuropsychiatric diseases. However, most existing methods yield unsatisfactory results due to blurred boundaries and complex structures. Improving the segmentation ability requires the model to be explicit about the spatial localization and shape appearance of targets, which correspond to the low-frequency content features and the high-frequency edge features, respectively.

A Time-Saving Strategy to Generate Double Maternal Mutants by an Oocyte-Specific Conditional Knockout System in Zebrafish.

Maternal products are those mRNAs and proteins deposited during oogenesis, which play critical roles in controlling oocyte formation, fertilization, and early embryonic development. However, loss-of-function studies for these maternal factors are still lacking, mainly because of the prolonged period of transgenerational screening and technical barriers that prevent the generation of maternal (M) and maternal and zygotic (MZ) mutant embryos. By the transgenic expression of multiple sgRNAs targeting a single gene of interest in the background of a transgenic line Tg(:) with oocyte-specific expression, we have successfully obtained maternal or maternal-zygotic mutant for single genes in F1 embryos.

Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish.

Maternal products are exclusive factors to drive oogenesis and early embryonic development. As disrupting maternal gene functions is either time-consuming or technically challenging, early developmental programs regulated by maternal factors remain mostly elusive. We provide a transgenic approach to inactivate maternal genes in zebrafish primary oocytes.

Visualisation of flooding along an unvegetated, ephemeral river using Google Earth Engine: Implications for assessment of channel-floodplain dynamics in a time of rapid environmental change.

Given rapid environmental change, the development of new, data-driven, interdisciplinary approaches is essential for improving assessment and management of river systems, especially with respect to flooding. In the world's extensive drylands, difficulties in obtaining field observations of major hydrological events mean that remote sensing techniques are commonly used to map river floods and assess flood impacts. Such techniques, however, are dependent on available cloud-free imagery during or immediately after peak discharge, and single images may omit important flood-related hydrogeomorphological events.

[Usage and efficacy of timolol maleate eye drops in treatment of superficial infantile hemangioma].

To determine drug dose and usage of timolol maleate eye drops in the treatment of superficial infantile hemangioma.
 Methods: A total of 250 superficial hemangioma infants were recruited and assigned into 5 groups (n=50 for each group): an external application group and 4 exterior coating groups (2, 4, 6, 8 times per day). We evaluated the therapeutic effect of different methods for drug application (external application or exterior coating) and the frequency for drug administration on superficial infantile hemangioma.

[Endovascular treatment for venous malformations with three-point anhydrous ethanol injection].

Objective: To explore the safe method with anhydrous ethanol injection in the treatment of venous malformation.


Methods: A total of 96 patients with venous malformation were conducted anhydrous ethanol injection for 245 times through percutaneous puncture by three-point method. The complications were observed.

MacroH2A suppresses the proliferation of the B16 melanoma cell line.

MacroH2A is the most frequently altered histone, which participates in cancer progression. Increasing evidence demonstrates that cancer progression could be regulated by macroH2A by affecting the cell cycle. In the present study, it was demonstrated that macroH2A suppresses melanoma cell progression and the molecular mechanisms underlying this process were examined.