Single-holed cobalt - nitrogen - carbon hollow structure with oxidase-mimicking activity for the chemiluminescence determination of β - galactosidase activity.

A single-holed cobalt - nitrogen - carbon (Co - N - C) hollow structure nanozyme has been fabricated by in situ growth of zeolitic imidazolate framework (ZIF - 67) on the polystyrene (PS) sphere and following treatment by high-temperature carbonization. The Co - N - C nanostructure mimics the activity of oxidase and can activate O into reactive oxygen species (ROS), giving a remarkable enhancement on the chemiluminescence (CL) signal of luminol - O reaction. The Co - N - C oxidase mimic has further been exploited in the biosensing field by the determination of the activity of β - galactosidase (β - gal).

An Aggregation-Induced Emission Nanosensor for Real-Time Chemiluminescent Sensing of Light-Independent Intracellular Singlet Oxygen.

Characterizing the transient ultratrace light-independent intracellular singlet oxygen (O), which plays a vital role in multiple biological processes in living organisms, brings about tremendous help for understanding the nature of O-mediated or related bioevents. Nevertheless, an approach to detect the light-independent intracellular O is hard to find. Herein, we developed a chemiluminescent nanosensor by compacting a great number of TPE-N(Ph)-DBT-PH molecules in one nanostructure via autoaggregation.

Real-Time Mapping of Ultratrace Singlet Oxygen in Rat during Acute and Chronic Inflammations via a Chemiluminescent Nanosensor.

Sensing nonradiation-induced singlet oxygen ( O ) in whole-animal is deemed as one of the most challenging tasks in noninvasive techniques due to the µs level lifetime of O and quenching by numerous reductants in tissues. Here a distinct chemiluminescent (CL) nanosensor (NTPE-PH) that boasts ultrahigh concentrated CL units in one nanoparticle is reported. Taking advantage of the intramolecular energy transfer mechanism that promises high energy transfer efficiency and the aggregation-induced emission behavior that guarantees high CL amplification, the NTPE-PH sensor is sensitive to a nm level O .

A novel thermometer-type hydrogel senor for glutathione detection.

A thermometer-type visual sensor for glutathione (GSH) sensing was developed with stimulus-responsive fluorescent hydrogel which was obtained by using 5, 6-bicarboxylic fluorescein crossli`nked partly ammoniated polyacrylamide. Various experimental parameters such as the particle size of hydrogel, buffer solution and swelling time were optimized. It is accessible to measure the volume change of hydrogel with the sensor by reading the graduation on a pipette like thermometer with naked eye.

Self-Catalyzing Chemiluminescence of Luminol-Diazonium Ion and Its Application for Catalyst-Free Hydrogen Peroxide Detection and Rat Arthritis Imaging.

We report the unique self-catalyzing chemiluminescence (CL) of luminol-diazonium ion (N-luminol) and its analytical potential. Visual CL emission was initially observed when N-luminol was subjected to alkaline aqueous HO without the aid of any catalysts. Further experimental investigations found peroxidase-like activity of N-luminol on the cleavage of HO into OH radical.

A visual volumetric hydrogel sensor enables quantitative and sensitive detection of copper ions.

We propose a visual volumetric sensor with 5,6-dicarboxylic fluorescein cross-linked amine-functionalized polyacrylamide hydrogel. The sensor undergoes volume response to Cu(2+) ions at the μM level, which enables naked-eye quantitative detection by reading the graduation on a pipette.

A siphonage flow and thread-based low-cost platform enables quantitative and sensitive assays.

For pump-free, material abundant, portable, and easy-to-operate low-cost microfluidics, a siphonage flow microfluidic thread-based analytical device (S-μTAD) platform enabling quantitative and sensitive assays was designed. Renewable and continuous siphonage flow allowed replicate sampling and detection on one channel/device, obviating some possible inconsistencies among channels or devices. Y-shaped channels were fabricated with polyester cotton blend thread, due to its greater chemiluminescent sensitivity in comparison with that of cotton and polyester threads.

A high-performance liquid chromatography:chemiluminescence method for potential determination of vardenafil in dietary supplement.

A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H(3)PO(4) and ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K(3)Fe(CN)(6) reaction in alkaline medium.

Three-dimensional positron emission tomography/computed tomography analysis of 13NO3- uptake and 13N distribution in growing kohlrabi.

We report the application of three-dimensional positron emission tomography/computed tomography (PET/CT) for the analysis of (13)NO(3)(-) uptake and (13)N distribution in growing kohlrabi. The analytical procedures, equipment parameters, and image reconstruction mode for plant imaging were tested and selected. (13)N in growing kohlrabi plants was imaged versus time using both PET movies and PET/CT tomograms.

Flow-injection chemiluminescence simultaneous determination of cobalt(II) and copper(II) using partial least squares calibration.

A flow-injection chemiluminescence (CL) system is proposed for simultaneous determination of Co(2+) and Cu(2+) using partial least squares (PLS) calibration. This method is based on the fact that both Co(2+) and Cu(2+) catalyse the CL reaction of luminol-H(2)O(2), and that their kinetic characteristics of Co(2+) and Cu(2+) are significantly different in the luminol-H(2)O(2) system. The CL intensity was measured and recorder at different reaction times of luminol-H(2)O(2)Co(2+)Cu(2+), and the obtained data were processed by the chemometric approach of partial least squares.

Utilizing multibubble sonophotoluminscence for calcium quantitative analysis in pharmaceuticals.

Sonoluminescence can photoexcite fluorescent solutes yielding sonophotoluminescence (SPL). The possibility of applying multibubble sonophotoluminescence (MBSPL) for chemical quantitative analysis is examined in this work. Special devices for MBSPL quantitative detection are designed and fabricated.

Flow injection chemiluminescence detection and solvent extraction for human skin ointment dexamethasone acetate absorption analysis and the reaction mechanism study.

A flow-injection chemiluminescence (CL) method coupled with solvent extraction for the determination of dexamethasone acetate (DA) in ointment was presented in this paper. It was based on the enhancing effect of the studied drug on CL emission of luminol-K(3)Fe(CN)(6) system. This method has the advantages of simple sample treatment, cheap instrumentation and rapid detection.

Determination of phenol at ng l-1 level by flow-injection chemiluminescence combined with on-line solid-phase extraction.

In this paper, a simple flow-injection chemiluminescence (CL) system combined with on-line solid-phase extraction is presented to determine phenol. This method is based on the enhancement effect of phenol on the luminol-K3Fe(CN)6 CL system. The solid-phase extraction promised the high sensitivity and improved selectivity of CL detection.

Chemometrics-assisted simultaneous determination of cobalt(II) and chromium(III) with flow-injection chemiluminescence method.

In this paper, a flow-injection chemiluminescence (CL) system is proposed for simultaneous determination of Co(II) and Cr(III) with partial least squares calibration. This method is based on the fact that both Co(II) and Cr(III) catalyze the luminol-H(2)O(2) CL reaction, and that their catalytic activities are significantly different on the same reaction condition. The CL intensity of Co(II) and Cr(III) was measured and recorded at different pH of reaction medium, and the obtained data were processed by the chemometric approach of partial least squares.

Simultaneous determination of rifampicin and isoniazid by continuous-flow chemiluminescence with artificial neural network calibration.

In this paper a continuous-flow chemiluminescence (CL) system with artificial neural network calibration is proposed for simultaneous determination of rifampicin and isoniazid. This method is based on the different kinetic spectra of the analytes in their CL reaction with alkaline N-bromosuccinimide as oxidant. The CL intensity was measured and recorded every second from 1 to 300 s.

A micro-chemiluminescence determination of cyanide in whole blood.

A reactant volume self-controlled micro-device was presented and applied to the flow injection chemiluminescence (CL) for determination of cyanide in whole blood. A mini distiller was fabricated for cyanide extraction from the blood samples with the extraction efficiencies of cyanide > or = 98%. A fluidic control platform with air driving was fabricated.

An on-line galvanic cell-generated electrochemiluminescence and flow injection determination of calcium in milk and vegetables.

An on-line Ag/Al galvanic cell was studied and employed to generate electrochemiluminescence (ECL) of calcein blue. The potential of the galvanic cell could be adjusted by varying the components of flow reagent or by using different metals to substitute for Ag or Al. The reported cell exhibited perfect capability of supplying a stable potential for ECL generation.