Mettl3/Eed/Ythdc1 regulatory axis controls endometrial receptivity and function.

The regulatory mechanism between N6-methyladenosine (mA) RNA methylation and histone modification in endometrial receptivity remains poorly understood. In this study, we depict that RIF induced mA and Mettl3 level restrain, affecting H3K27me3 modification and chromatin accessibility. We show that Mettl3 deletion in the endometrium alters mRNA mA methylation via Eed interaction.

Genome-Wide Scans for Selection Signatures in Haimen Goats Reveal Candidate Genes Associated with Growth Traits.

Understanding the genetic characteristics of indigenous goat breeds is vital for their conservation and breeding. Haimen goats, native to China's Yangtze River Delta, possess distinctive traits such as white hair, moderate growth rate, high-quality meat, and small body size. However, knowledge regarding the genetic structure and germplasm characteristics of Haimen goats remains limited.

Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples.

The most effective method for mapping N6-methyladenosine (mA) is mA RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-mA antibody being a crucial determinant. However, comprehensive research on anti-mA antibody selection and optimal concentrations for different tissues has been limited.

The N6-methyladenosine landscape of ovarian development and aging highlights the regulation by RNA stability and chromatin state.

The versatile epigenetic modification known as N6-methyladenosine (mA) has been demonstrated to be pivotal in numerous physiological and pathological contexts. Nonetheless, the precise regulatory mechanisms linking mA to histone modifications and the involvement of transposable elements (TEs) in ovarian development and aging are still not completely understood. First, we discovered that mA modifications are highly expressed during ovarian aging (OA), with significant contributions from decreased mA demethylase FTO and overexpressed mA methyltransferase METTL16.

RNA methylation in neurodevelopment and related diseases.

Biological development and genetic information transfer are governed by genetic, epigenetic, transcriptional, and posttranscriptional mechanisms. RNA methylation, the attachment of methyl (-CH ) groups to RNA molecules, is a posttranscriptional modification that has gained increasing attention in recent years because of its role in RNA epitranscriptomics. RNA modifications (RMs) influence various aspects of RNA metabolism and are involved in the regulation of diverse biological processes and diseases.

Increased Expression of PDGFA Is Associated With Poor Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.

Background: Platelet-derived growth factor A (PDGFA) has been shown to be upregulated in several tumors, contributing to their malignant phenotypes. However, its expression and function in head and neck squamous cell carcinoma (HNSC) are not clearly understood. Thus, we aimed to evaluate this issue using bioinformatic analyses and primary experimental validation.

Tiaogan Bushen Xiaoji Formula Enhances the Sensitivity of Estrogen Receptor- Positive Breast Cancer to Tamoxifen by Inhibiting the TGF-β/SMAD Pathway.

Background: The resistance to endocrine therapy can lead to recurrence and metastasis of breast cancer (BC), affecting the survival period. Tiaogan Bushen Xiaoji (TGBSXJ) Formula, a traditional Chinese medicine (TCM) decoction, has been widely used in the treatment of estrogen receptor-positive (ER) BC. However, the underlying mechanism of TGBSXJ Formula in ERBC treatment has not been totally elucidated.

Construction and assessment of an angiogenesis-related gene signature for prognosis of head and neck squamous cell carcinoma.

Objective: Angiogenesis-associated genes (AAGs) play a critical role in cancer patient survival. However, there are insufficient reports on the prognostic value of AAGs in head and neck squamous cell carcinoma (HNSC). Therefore, this study aimed to investigate the correlation between AAG expression levels and survival in HNSC patients, explore the predictive value of signature genes and lay the groundwork for future in-depth research.

NSUN2-Mediated mC Methylation Impairs Endometrial Receptivity.

Impaired endometrial decidualization is the primary cause of recurrent implantation failure (RIF). RNA methylation modification, especially NSUN family mediated mC, is crucial for various physiological events, such as maternal-to-zygotic transition, gametogenesis, embryonic development, organismal lifespan, and cell cycle. However, the regulatory mechanisms between NSUN family mediated mC modification and RIF remain unknown.

A low-cost, low-input method establishment for m6A MeRIP-seq.

N6-methyladenosine (m6A) is a highly prevalent modification found in mammal mRNA molecules that plays a crucial role in the regulation of cellular function. m6A RNA immunoprecipitation sequencing (MeRIP-seq) has been frequently used in transcriptomics research to identify the location of m6A. MABE572 (Millipore) is the most widely utilized and efficient anti-m6A antibody for MeRIP-seq.

Transplantation of olfactory ensheathing cells can alleviate neuroinflammatory responses in rats with trigeminal neuralgia.

Trigeminal neuralgia (TN) is a common form of facial pain, which primarily manifests as severe pain similar to facial acupuncture and electric shock. Olfactory ensheathing cells (OECs) are glial cells with high bioactivity; these cells are essential for the periodic regeneration of the olfactory nerve and have been utilized for the repair of nerve injuries. A member of the P2X receptor family, P2X7R, is an ion channel type receptor that has been confirmed to participate in various pain response processes.

Deep learning-based vortex decomposition and switching based on fiber vector eigenmodes.

Structured optical fields, such as cylindrical vector (CV) and orbital angular momentum (OAM) modes, have attracted considerable attention due to their polarization singularities and helical phase wavefront structure. However, one of the most critical challenges is still the intelligent generation or precise control of these modes. Here, we demonstrate the first simulation and experimental realization of decomposing the CV and OAM modes by reconstructing the multi-view images of projected intensity distribution.

[Pollution Characteristics of PM Chemical Composition in Zhejiang Province].

To investigate the seasonal and regional pollution characteristics of PM chemical composition in Zhejiang province, this study was based on manual sampling monitoring data from 11 sampling sites of four regions in Zhejiang province from October 1, 2019 to September 30, 2020. The results showed that during the observation period, the average (PM) of the four regions ranged from 34.3 μg·m to 46.

ATP ion channel P2X purinergic receptors in inflammation response.

Different studies have confirmed that P2X purinergic receptors play a key role in inflammation. Activation of P2X purinergic receptors can release inflammatory cytokines and participate in the progression of inflammatory diseases. In an inflammatory microenvironment, cells can release a large amount of ATP to activate P2X receptors, open non-selective cation channels, activate multiple intracellular signaling, release multiple inflammatory cytokines, amplify inflammatory response.

Tailoring chiral optical properties by femtosecond laser direct writing in silica.

An object that possesses chirality, that is, having its mirror image not overlayed on itself by rotation and translation, can provide a different optical response to a left- or right-handed circular polarized light. Chiral nanostructures may exhibit polarization-selective optical properties that can be controlled for micro-to-nano optical element engineering. An attractive way to induce such complex nanostructures in three-dimension in glass is femtosecond laser direct writing.

Femtosecond laser direct writing multilayer chiral waveplates with minimal linear birefringence.

Chirality transfer from femtosecond laser direct writing in achiral transparent materials mainly originates from the interplay between anisotropic nanogratings and mechanical stress with non-parallel and non-perpendicular (oblique) neutral axes. Yet, the laser fabrication simultaneously induces non-negligible linear birefringence. For precise manipulation of circular polarization properties, as well as to unlock the full functionality, we report here a geometry-inspired multilayer method for direct writing of chiral waveplates with minimal linear birefringence.

RNA-methyltransferase Nsun5 controls the maternal-to-zygotic transition by regulating maternal mRNA stability.

Background: RNA modification-induced ovarian dysgenesis appears to be necessary for ovary development. However, how m C (5-methylcytosine)-coordinating modificatory transcripts are dynamically regulated during oogenesis, and ovarian development is unknown. The purpose of this study was to determine whether NOP2/Sun RNA methyltransferase 5 (Nsun5) deletion leads to suppression of ovarian function and arrest of embryonic development.

Observation on the efficacy of sublingual immunotherapy with dust mite allergen for perennial allergic rhinitis and the mechanism of action on ILCs with ILC1s and ILC2s and ILC3s.

Background: Allergic rhinitis (AR) is considered to be 1 of the most difficult diseases to treat globally. It has a serious impact on the quality of life and social economy of patients and has become an important global health problem. Several drugs have been recommended to treat AR, but their effectiveness and mechanism of action in these patients remain unclear.

Distributions and sources of traditional and emerging per- and polyfluoroalkyl substances among multiple environmental media in the Qiantang River watershed, China.

The presence of per- and polyfluoroalkyl substances (PFASs) in the food chain poses a threat to human health. Water and the atmosphere are the major transport pathways for PFASs in the environment, while water, soil and sediment are sinks. Herein, the concentrations and distributions of traditional and emerging PFASs in multi-environmental media samples in the Qiantang River watershed were comprehensively investigated.

Olfactory Ensheathing Cells Alleviate Facial Pain in Rats with Trigeminal Neuralgia by Inhibiting the Expression of P2X7 Receptor.

Trigeminal neuralgia (TN) is a common facial neuropathic pain that is mainly characterized by spontaneous or induced needling or electric shock pain in the innervation area of the trigeminal nerve. It is also referred to as "the cancer that never dies". The olfactory ensheathing cell (OEC) is a special glial cell in the nervous system that has a strong supportive function in nerve regeneration.

The Role of mA on Female Reproduction and Fertility: From Gonad Development to Ovarian Aging.

The growth and maturation of oocyte is accompanied by the accumulation of abundant RNAs and posttranscriptional regulation. N6-methyladenosine (mA) is the most prevalent epigenetic modification in mRNA, and precisely regulates the RNA metabolism as well as gene expression in diverse physiological processes. Recent studies showed that mA modification and regulators were essential for the process of ovarian development and its aberrant manifestation could result in ovarian aging.

Integrity of cell-free DNA in maternal plasma extracellular vesicles as a potential biomarker for non-invasive prenatal testing.

Objective: Large proportions of cell-free DNA (cfDNA) in plasma are localized in extracellular vesicles (EVs), which are secreted from placental cells. This study was conducted to reveal the integrity pattern of cfDNA in maternal plasma EVs (evcfDI) across gestation, and explore if evcfDI could be a potential biomarker in screening for aneuploid fetus in non-invasive prenatal testing (NIPT).

Methods: A total of 180 maternal plasma samples were collected during NIPT.

Vortex soliton oscillation in a mode-locked laser based on broadband long-period fiber grating.

Optical vortex beams (OVBs) have attracted much attention in diverse applications and spatiotemporal mode locking for optical soliton formation. In this Letter, a compact mode-locked (ML) vortex fiber laser is demonstrated based on a broadband long-period fiber grating near the dispersion turnaround point, where the group velocities between the core modes of ${{\rm LP}_{01}}$ and ${{\rm LP}_{11}}$ in a two-mode fiber are matched. The OVB pulses with first-order orbital angular momentum are oscillated through broadband mode conversion inside the cavity.

Human placental mesenchymal stem cells ameliorate chemotherapy-induced damage in the testis by reducing apoptosis/oxidative stress and promoting autophagy.

Background: The side effects of busulfan on male reproduction are serious, so fertility preservation in children undergoing busulfan treatment is a major worldwide concern. Human placental mesenchymal stem cells (hPMSCs) have advantages such as stable proliferation and lower immunogenicity that make them an ideal material for stimulating tissue repair, especially restoring spermatogenesis. The protective effects of hPMSCs in busulfan-induced Sertoli cells and in busulfan-treated mouse testes have not been determined.