Rapid and dual-mode nitrite detection with improved sensitivity by nanointerface-regulated ultrafast Griess assay.

Background: The rapid and sensitive detection of nitrite is important to human health protection due to its carcinogenic and teratogenic risks with excessive intake. The Griess assay is widely applied for the design of nitrite detection system. However, its relatively slow reaction kinetics and sole colorimetry mode might limit it's the sensitivity and practical application.

plecoglossicida infection induces neutrophil autophagy-driven NETosis in large yellow croaker .

Neutrophil extracellular traps (NETs) are crucial for the immune defense of many organisms, serving as a potent mechanism for neutrophils to capture and eliminate extracellular pathogens. While NETosis and its antimicrobial mechanisms have been well studied in mammals, research on NETs formation in teleost fish remains limited. In this study, we used the large yellow croaker () as the study model to investigate NETosis and its role in pathogen defense.

In-Situ Sulfuration of Ni(OH) to Heterostructured NiS/Ni(OH)@Ni Catalyst for Efficient Water Splitting.

The exploring and developing non-precious transition metal-based catalysts for practical water electrolysis with the low cost, high efficiency and easy macroscopic preparation was still a challenge. Herein, NiS/Ni(OH) heterojunction with different sulfuration time was proposed and hydrothermally synthesized using a simple two-step approach, which served as a bifunctional electrocatalyst for water splitting in alkaline solution at industrial temperature. Among these catalysts, NiS/Ni(OH)-5h displayed the smallest overpotentials (237 mV@100 mA cm and 360 mV@100 mA cm) for OER and HER at room temperature, along with low Tafel slopes of 62.

Emerging role of Jumonji domain-containing protein D3 in inflammatory diseases.

Jumonji domain-containing protein D3 (JMJD3) is a 2-oxoglutarate-dependent dioxygenase that specifically removes transcriptional repression marks di- and tri-methylated groups from lysine 27 on histone 3 (H3K27me2/3). The erasure of these marks leads to the activation of some associated genes, thereby influencing various biological processes, such as development, differentiation, and immune response. However, comprehensive descriptions regarding the relationship between JMJD3 and inflammation are lacking.

Nanotechnologies in ceramic electrochemical cells.

Although they are emerging technologies for achieving high-efficiency and green and eco-friendly energy conversion, ceramic electrochemical cells (CECs), solid oxide electrolysis cells (SOECs) and fuel cells (SOFCs), are still fundamentally limited by their inferior catalytic activities at low temperature, poor thermo-mechanical stability, high material cost, The materials used in electrolytes and electrodes, which are the most important components in CECs, are highly associated with the cell performances. Therefore, rational design of electrolytes and electrodes with excellent catalytic activities and high stabilities at relatively low cost is a meaningful and valuable approach for the development of CECs. Nanotechnology is a powerful tool for improving the material performances in CECs owing to the favourable effects induced by the nanocrystallization of electrolytes and electrodes.

Role of mTORC1 Signaling in Regulating the Immune Function of Granulocytes in Teleost Fish.

Granulocytes are crucial innate immune cells that have been extensively studied in teleost fish. Studies in mammals have revealed that mechanistic target of rapamycin complex 1 (mTORC1) signaling acts as a significant immune regulatory hub, influencing granulocyte immune function. To investigate whether mTORC1 signaling also regulates the immune function of granulocytes in teleost fish, we established a model of RAPA inhibition of the mTORC1 signaling pathway using granulocytes from largemouth bass ().

Sulfidation and NaOH etching in CoFeAl LDH evolved catalysts for an efficient overall water splitting in an alkaline solution.

In this study, a hierarchical interconnected porous metal sulfide heterostructure was synthesized from CoFeAl layered double hydroxides (LDHs) by a two-step hydrothermal process (sulfidation and a NaOH etching process). Among the as-made samples, the CoFeAl-T-NaOH electrode exhibited excellent oxygen and hydrogen evolution reaction catalytic activities with overpotentials of 344 mV and 197 mV at the current density of 100 mA cm, respectively. Meanwhile, small Tafel slopes of 57.

Regulatory roles of cytokines in T and B lymphocytes-mediated immunity in teleost fish.

T and B lymphocytes (T and B cells) are immune effector cells that play critical roles in adaptive immunity and defend against external pathogens in most vertebrates, including teleost fish. In mammals, the development and immune response of T and B cells is associated with cytokines including chemokines, interferons, interleukins, lymphokines, and tumor necrosis factors during pathogenic invasion or immunization. Given that teleost fish have evolved a similar adaptive immune system to mammals with T and B cells bearing unique receptors (B-cell receptors (BCRs) and T-cell receptors (TCRs)) and that cytokines in general have been identified, whether the regulatory roles of cytokines in T and B cell-mediated immunity are evolutionarily conserved between mammalians and teleost fish is a fascinating question.

Expression analysis of Igs and mucosal immune responses upon SVCV infection in common carp ().

The immunoglobulin (Ig) is a crucial component of adaptive immune system in vertebrates including teleost fish. Here complete cDNA sequence of IgD heavy chain gene from common carp () was cloned and analyzed. The full-length cDNA of IgD heavy chain gene contained an open reading frame (ORF) of 2460 bp encoding 813 amino acids.

Conserved Role of mTORC1 Signaling in B Cell Immunity in Teleost Fish.

Mammalian studies have demonstrated that B cell immune responses are regulated by mechanistic target of rapamycin complex 1 (mTORC1) signaling. Teleost fish represent the oldest living bony vertebrates that contain bona fide B cells. So far, whether the regulatory mechanism of mTORC1 signaling in B cells occurred in teleost fish is still unknown.

Teleost swim bladder, an ancient air-filled organ that elicits mucosal immune responses.

The air-filled organs (AOs) of vertebrates (lungs and swim bladders) have evolved unique functions (air-breathing or buoyancy control in water) to adapt to different environments. Thus far, immune responses to microbes in AOs have been described exclusively in the lungs of tetrapods. Similar to lungs, swim bladders (SBs) represent a mucosal surface, a feature that leads us to hypothesize a role for SB in immunity.

Molecular Characterization and Expression Analysis of Intercellular Adhesion Molecule-1 (ICAM-1) Genes in Rainbow Trout () in Response to Viral, Bacterial and Parasitic Challenge.

The intercellular adhesion molecule-1 (ICAM-1), known as CD54, is a transmembrane cell surface glycoprotein that interacts with two integrins (i.e., LFA-1 and Mac-l) important for trans-endothelial migration of leukocytes.

Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection.

The skin of vertebrates is the outermost organ of the body and serves as the first line of defense against external aggressions. In contrast to mammalian skin, that of teleost fish lacks keratinization and has evolved to operate as a mucosal surface containing a skin-associated lymphoid tissue (SALT). Thus far, IgT representing the prevalent Ig in SALT have only been reported upon infection with a parasite.

A heterogeneous parasitic-mutualistic model of mistletoes and birds on a periodically evolving domain.

In this paper, a parasitic-mutualistic model of mistletoes and birds defined in a heterogneous and periodically evolving domain is comprehensively investigated to reveal some new dynamical phenomena caused by the domain evolution. By establishing the core spatial-temporal risk index and for birds population and mistletoes-birds population, respectively, the fundamental extinction, persistence and coexistence behaviors are studied, and distinguished by such indexes. Moreover, the impact of domain evolution on the viability of mistletoes is examined as well, and it is concluded that the average expansion of the domain can enhance mistletoes' transmission capability, therefore, pro-mote the viability of mistletoes, and vice versa.

Expression analysis of taste receptor genes (T1R1, T1R3, and T2R4) in response to bacterial, viral and parasitic infection in rainbow trout, Oncorhynchus mykiss.

Emerging evidence suggests that bitter and sweet Taste receptors (TRs) in the airway are important sentinels of innate immunity. TRs are G protein-coupled receptors that trigger downstream signaling cascades in response to activation of specific ligands. Among them, the T1R family consists of three genes: T1R1, T1R2, and T1R3, which function as heterodimers for sweet tastants and umami tastants.

Viral-Infected Change of the Digestive Tract Microbiota Associated With Mucosal Immunity in Teleost Fish.

The digestive tract is a unique series of organs that is inhabited by a range of commensal microbes while also exposed to an overwhelming load of dietary antigens. It is widely known that mammals have evolved complex and efficient immune strategies to protect the mucosa of the digestive tract. However, in the early vertebrates, the roles of mucosal immune defense and microbial communities in the different segments of the digestive tract are not well-understood.

Pharyngeal Immunity in Early Vertebrates Provides Functional and Evolutionary Insight into Mucosal Homeostasis.

The pharyngeal organ is located at the crossroad of the respiratory and digestive tracts in vertebrate, and it is continuously challenged by varying Ags during breathing and feeding. In mammals, the pharyngeal mucosa (PM) is a critical first line of defense. However, the evolutionary origins and ancient roles of immune defense and microbiota homeostasis of PM are still unknown.

Convergent Evolution of Mucosal Immune Responses at the Buccal Cavity of Teleost Fish.

The buccal mucosa (BM) is a critical first line of defense in terrestrial animals. To gain further insights into the evolutionary origins and primordial roles of BM in teleosts here we show that rainbow trout, a teleost fish, contains a diffuse mucosal associated lymphoid tissue (MALT) within its buccal cavity. Upon parasite infection, a fish immunoglobulin specialized in mucosal immunity (sIgT) was induced to a high degree, and parasite-specific sIgT responses were mainly detected in the buccal mucus.

An Efficient Electron-Blocking Interlayer Induced by Metal Ion Diffusion for SOFC Based on Y-Doped Ceria Electrolyte.

To suppress the internal electronic leakage at ceria-based electrolyte, a novel electron-blocking layer consisting of doped BaCeYO was fabricated in situ at the interface of Ba-containing anode and Y-doped ceria electrolyte. The anode-supported full cell based on YCeO (YDC20) electrolyte presents a remarkable peak power density of 814 mW/cm as well as an open-circuit voltage of 1.0 V at 650 °C, which are much higher than those of the cells with GdCeO (GDC10) electrolyte (453 mW/cm at 650 °C) and BaCeYO|YCeO (BCY|YDC20) bilayered electrolyte (419 mW/cm at 650 °C).

Upconversion luminescence of BaLa(PO):Yb-Er/Tm phosphors for optimal temperature sensing.

To explore new upconversion (UC) luminescent materials for optical temperature sensing, a series of BaLa(PO):0.1Yb,xEr (abbreviated as BLP:0.1Yb, xEr, 0.