A novel strategy for enhancing anaerobic biodegradation of an anthraquinone dye reactive blue 19 with resuscitation-promoting factors.

Anaerobic process has been widely applied as a cost-effective method for textile wastewater treatment. However, many bacteria exhibit low metabolic activity in unfavorable conditions due to the entry into a viable but non-culturable (VBNC) state. Thus, in this study, a novel method of using resuscitation-promoting factors (Rpfs), which has been proven to resuscitate and stimulate the growth of VBNC bacteria, is explored to enhance the degradation of the anthraquinone dye reactive blue 19 (RB19) in the anaerobic process.

Effective decolorization of anthraquinone dye reactive blue 19 using immobilized Bacillus sp. JF4 isolated by resuscitation-promoting factor strategy.

Given the highly complex recalcitrant nature of synthetic dyes, biological treatment of textile wastewater using efficient bacterial species is still considered as an environmentally friendly manner. In this study, a reactive blue 19 (RB19)-degrading strain, Bacillus sp. JF4, which was isolated by resuscitation-promoting factor (Rpf) strategy, was immobilized into polyvinyl alcohol-calcium alginate-activated carbon beads (JF4-immobilized beads) for RB19 decolorization.

Resuscitation, isolation and immobilization of bacterial species for efficient textile wastewater treatment: A critical review and update.

Given highly complex and recalcitrant nature of synthetic dyes, textile wastewater poses a serious challenge on surrounding environments. Until now, biological treatment of textile wastewater using efficient bacterial species is still considered as an environmentally friendly and cost-effective approach. The advances in resuscitating viable but non-culturable (VBNC) bacteria via signaling compounds such as resuscitation-promoting factors (Rpfs) and quorum sensing (QS) autoinducers, provide a vast majority of potent microbial resources for biological wastewater treatment.

Aerobic degradation of 3,3',4,4'-tetrachlorobiphenyl by a resuscitated strain Castellaniella sp. SPC4: Kinetics model and pathway for biodegradation.

Resuscitated strains which were obtained by addition of resuscitation promoting factor (Rpf) could provide a vast majority of microbial source for obtaining highly efficient polychlorinated biphenyl (PCB)-degrading bacteria. In this study, the Castellaniella sp. strain SPC4 which was resuscitated by Rpf addition showed the highest efficiency in degradation of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) among the resuscitated and non-resuscitated isolates.