Transcriptomic analysis of the inhibition mechanisms against by antibacterial aptamer B4.

is a common bacterial pathogen in aquaculture, often leading to visceral white spot disease in large yellow croakers (). Previous studies have found that certain aptamers show an efficient antibacterial effect against this pathogen. In this study, we analyzed the transcriptome of to get insights into the antibacterial and inhibitions mechanisms following exposure to the aptamer B4.

Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe.

DNA helicases play a pivotal role in maintaining genome integrity by unwinding the DNA double helix and are often considered promising targets for drug development. However, assessing specific DNA helicase activity in living cells remains challenging. Herein, the first anchor-embedded duplex (ATED) probe, 17GC, is constructed to uniquely monitor the unwinding activity of Werner syndrome helicase (WRN), a clinical anticancer target.

The functional role of N-link glycosylation in a novel cellobiohydrolase II (LsCel6A) from a white-rot fungus Lentinus sp. WR2.

White-rot fungi produce a wide spectrum of lignocellulose-degradation enzymes, which can be used in bioenergy, bioremediation, and other industrial applications. This study identified a cellobiohydrolase II (Cel6A, GH6 cellobiohydrolase, EC 3.2.

A rapid and highly sensitive immunosorbent assay to monitor helicases unwinding diverse nucleic acid structures.

Helicases are crucial enzymes in DNA and RNA metabolism and function by unwinding particular nucleic acid structures. However, most convenient and high-throughput helicase assays are limited to the typical duplex DNA. Herein, we developed an immunosorbent assay to monitor the Werner syndrome (WRN) helicase unwinding a wide range of DNA structures, such as a replication fork, a bubble, Holliday junction, G-quadruplex and hairpin.

Design, synthesis and evaluation of N3-substituted quinazolinone derivatives as potential Bloom's Syndrome protein (BLM) helicase inhibitor for sensitization treatment of colorectal cancer.

The homologous recombination repair (HRR) pathway is critical for repairing double-strand breaks (DSB). Inhibition of the HRR pathway is usually considered a promising strategy for anticancer therapy. The Bloom's Syndrome Protein (BLM), a DNA helicase, is essential for promoting the HRR pathway.

Bioinspired High-Strength Montmorillonite-Alginate Hybrid Film: The Effect of Different Divalent Metal Cation Crosslinking.

The natural nacre has a regular ordered layered structure of calcium carbonate tablets and ion crosslinking proteins stacked alternately, showing outstanding mechanical properties. Inspired by nacre, we fabricated different divalent metal cation-crosslinked montmorillonite-alginate hybrid films (MMT-ALG-X2+; X2+ = Cu2+, Cd2+, Ba2+, Ca2+, Ni2+, Co2+ or Mn2+). The effect of ionic crosslinking strength and hydrogen bond interaction on the mechanical properties of the nacre-mimetics was studied.

Design, Synthesis, and Evaluation of New Quinazolinone Derivatives that Inhibit Bloom Syndrome Protein (BLM) Helicase, Trigger DNA Damage at the Telomere Region, and Synergize with PARP Inhibitors.

DNA damage response (DDR) pathways are crucial for the survival of cancer cells and are attractive targets for cancer therapy. Bloom syndrome protein (BLM) is a DNA helicase that performs important roles in DDR pathways. Our previous study discovered an effective new BLM inhibitor with a quinazolinone scaffold by a screening assay.

Emotional prosody perception and its association with pragmatic language in school-aged children with high-function autism.

Emotional prosody perception is essential for social communication, but it is still an open issue whether children with high-function autism (HFA) exhibit any prosodic perception deficits or experience selective impairments in recognizing the prosody of positive emotions. Moreover, the associations between prosody perception, pragmatic language, and social adaptation in children with HFA have not been fully explored. This study investigated whether emotional prosody perception for words and sentences in children with HFA (n=25, 6-11 years of age) differed from age-matched, typically developing children (TD, n=25) when presented with an emotional prosody identification task.