CRISPR/Cas12a-Triggered Visible-Light-Driven Photoelectrochemical Assay with Single-Nucleotide Resolution for Drug-Resistant Foodborne Detection.

The prevalence of foodborne pathogenic bacteria, especially drug-resistant strains, such as , poses serious threats to public health, highlighting the requirement for the development of rapid and precise detection methods. Herein, a CRISPR/Cas12a-triggered visible-light-driven photoelectrochemical (PEC) assay (CasPEC) was developed using a SiO-quenched BiVO/MoS p/n-type heterojunction as the photoactive material. The CRISPR/Cas12a recognition endowed the CasPEC assay with high specificity capable of resolving single-nucleotide polymorphisms (SNPs) and identifying SNP-involved drug-resistant bacteria.