Chronic inflammation is a factor in the development of cancer, and probiotics play a role in preventing or treating inflammation as an adjuvant therapy. To investigate potential probiotics for the prevention of colitis-associated colorectal cancer (CAC), H3-R2 and KLDS4.0325 were used to examine the effects on colon cancer cells and in an inflammation-related cancer animal model.
View Article and Find Full Text PDFIn this study, a novel homogeneous mannose-rich polysaccharide named EPS-1 from the fermentation broth of H4-2 was isolated and purified by anion exchange column chromatography and gel column chromatography. The primary structure of EPS-1 was analyzed by high-performance liquid chromatography, Fourier-transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance. The results indicated that EPS-1 had typical functional groups of polysaccharides.
View Article and Find Full Text PDFMultigrain products can prevent the occurrence of chronic noninfectious diseases such as hyperglycemia and hyperlipidemia. In this study, multigrain dough fermented by lactic acid bacteria (LAB) was used for the preparation of good-quality steamed multigrain bread, and its effects on type 2 diabetes were investigated. The results showed that the multigrain dough fermented with LAB significantly enhanced the specific volume, texture, and nutritional value of the steamed bread.
View Article and Find Full Text PDFProbiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC). In the present study, DSS-treated C57BL/6J mice were gavaged with H3-R2, B1 and C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively. We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
View Article and Find Full Text PDFJ Agric Food Chem
September 2022
Bifidobacteria are important mediators of immune system development within the gastrointestinal system and immunological homeostasis. The present study explored the anti-colitic activity of H3-R2 in a murine dextran sulfate sodium (DSS)-induced model of ulcerative colitis (UC). Moreover, this study offers novel insight regarding the molecular basis for the probiotic properties of H3-R2 by analyzing the underlying mechanisms whereby H3-R2-derived proteins affect the intestinal barrier.
View Article and Find Full Text PDFThe composition of the microbiome in the early stages of life can directly affect the health of developing infants, and prior evidence suggests that human milk oligosaccharides (HMO) are critical regulators in the maintenance of a healthy gut microbiota in infants. Herein, we conducted an analysis of the gut microbiota of 1-mo-old breastfed infants from Jining and Harbin, China, and a corresponding analysis of the HMO profiles in samples of maternal breast milk. Quantification of HMO was conducted via liquid chromatography-mass spectrometry, and bacterial DNA sequencing was employed for characterization of the fecal microbiota.
View Article and Find Full Text PDFis an important industrial lactic acid bacteria, which can be used as a starter culture for fermented milk and meat products. Many strains can produce bacteriocins with broad-spectrum antibacterial properties, heat stability, and easy to be hydrolyzed by protease in the process of food fermentation and metabolism, and their potential as biopreservative starter culture in the preservation of fermented food has been recognized. However, the high salt environment in the food matrix can affect bacteriocin production.
View Article and Find Full Text PDFThe microbiota is directly involved in the development and modulation of the intestinal immune system. In particular, members of the genus play a primary role in immune regulation. In the present study, H3-R2 was screened from 15 bifidobacterium strains by experiment, showing a positive tolerance to digestive tract conditions, adhesion ability to intestinal epithelial cells and a regulatory effect on immune cell activity.
View Article and Find Full Text PDF, a probiotic, has a high survival rate and high colonization ability in the gastrointestinal tract. Tolerance to the gastrointestinal environment and adhesion to intestinal epithelial cells by some species (excluding ) are related to /AI-2. Here, the role of in tolerance to simulated digestive juice (SDJ) and adhesion to Caco-2 cells by KLDS1.
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