Publications by authors named "Jiacai Yang"
Article Synopsis
- A study compared a new blood collection technique from mice, called inferior vena cava puncture under continuous cardiac perfusion (IVCP-UCCP), to the traditional cardiac puncture (CP) method.
- The results revealed that IVCP-UCCP collected over twice the amount of usable blood compared to CP, without affecting blood cell composition or viability.
- This improved method allows for more efficient use of limited blood resources in mice, offering benefits for both scientific research and ethical considerations, and could be applied to other small animal models as well.
Article Synopsis
- - STING is identified as a vital immune mediator that helps resolve inflammation and aids in skin repair by regulating the movement and function of macrophages during wound healing.
- - Mice lacking STING showed delayed wound healing due to persistent TNF-α+ leukocytes and impaired recruitment of macrophages, highlighting its role in controlling myeloid cell trafficking to wounds.
- - The study reveals that STING enhances macrophage migration through STAT3 activation and regulates chemokine production, suggesting potential therapeutic targets for enhancing wound healing and treating inflammatory diseases.
Article Synopsis
- Macrophages play dual roles in skin wound healing, switching from proinflammatory (M1) to anti-inflammatory (M2) states, which is crucial for effective recovery.
- The study identifies fibroblast-derived exosomes as key players that regulate this transition in macrophage activation without direct cell contact, enhancing their responsiveness to changes in the healing environment.
- Administering these exosomes can correct dysfunctional inflammation in chronic wounds, presenting a promising therapeutic avenue for improving healing in inflammatory conditions.
Dysregulated macrophage polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotypes underlies impaired cutaneous wound healing. This study reveals Vγ4 γδ T cells spatiotemporally calibrate macrophage trajectories during skin repair via sophisticated interferon-γ (IFN-γ) conditioning across multiple interconnected tissues. Locally within wound beds, infiltrating Vγ4 γδ T cells directly potentiate M1 activation and suppress M2 polarization thereby prolonging local inflammation.
View Article and Find Full Text PDFA Nanomedicine-Enabled Ion-Exchange Strategy for Enhancing Curcumin-Based Rheumatoid Arthritis Therapy.
Angew Chem Int Ed Engl
October 2023
Article Synopsis
- Curcumin is used to treat rheumatoid arthritis due to its ability to scavenge reactive oxygen species (ROS) and regulate the immune response, but its poor water solubility limits its effectiveness.
- Metal complexes of curcumin, like zinc-curcumin (Zn-Cur) and copper-curcumin (Cu-Cur), improve some properties, with Zn-Cur being more soluble and Cu-Cur exhibiting greater antioxidant activity.
- The proposed ion-exchange strategy involves using copper silicate nanoparticles to deliver Zn-Cur, which releases Cu and Zn in the acidic environment of arthritis, forming a more effective Cu-Cur that enhances anti-inflammatory responses and promotes bone cell development.
Background: P311, a highly conserved 8 kDa intracellular protein, has recently been reported to play an important role in aggravating hypertrophic scaring by promoting the differentiation and secretion of fibroblasts. Nevertheless, how P311 regulates the differentiation and function of fibroblasts to affect granulation tissue formation remains unclear. In this work, we studied the underlying mechanisms via which P311 affects fibroblasts and promotes acute skin wound repair.
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- The transition from the proinflammatory to prohealing phase is vital for skin wound repair and depends on the balance of M1 and M2 macrophage polarization.
- P311 is crucial in this process as it enhances the function of skin cells and promotes wound closure, but its role in macrophage polarization was previously unclear.
- Deficiency of P311 leads to reduced M2 macrophage polarization, impaired healing processes like angiogenesis and granulation tissue formation, and downregulates the IL-4 receptor, highlighting the importance of P311 in effective wound healing.
Article Synopsis
- γδ T cells are essential for the skin's immune defense against damage and infections, differing from conventional αβ T cells in their characteristics.
- In adult mice, key subsets of γδ T cells, like dendritic epidermal T cells (DETCs), Vγ4, and Vγ6, are crucial for skin wound repair through their coordination and interaction.
- The review explores the derivation, development, colonization, and activation of γδ T cells, focusing on their roles in both acute and chronic wound healing to inform research on human epidermal γδ T cells and potential skin treatment options.
Constructing nanomaterials mimicking the coordination environments of natural enzymes may achieve biomimetic catalysis. Here we construct a two-dimensional (2D) metal-organic framework (MOF) nanosheet catalyst as an artificial antioxidase for nanocatalytic rheumatoid arthritis treatment. The 2D MOF periodically assembles numbers of manganese porphyrin molecules, which has a metal coordination geometry analogous to those of two typical antioxidases, human mitochondrial manganese superoxide dismutase (Mn-SOD) and human erythrocyte catalase.
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- Efficient re-epithelialization is key for skin wound healing, and the balance of epidermal stem cells (EpSCs) and dendritic epidermal T cells (DETCs) influences this process, although the regulatory mechanism of DETCs on EpSCs is unclear.
- The study utilized normal and Tcrσ knockout mice, applying various analyses (e.g., flow cytometry, immunohistochemistry) to assess how DETCs and their exosomes (Exos) impact EpSCs and wound healing.
- Results indicated that DETCs significantly boost the proportion of specific EpSC markers and enhance proliferation and wound closure through their exosomes, with these
Article Synopsis
- Mesenchymal stem cells (MSCs) are being researched as a therapeutic option for healing injured tissues, with a focus on enhancing their healing abilities.
- The study highlights the importance of neuronal protein 3.1 (P311) in improving MSCs' function, showing that its increased expression boosts healing by reducing inflammation, raising IL10 levels, and promoting significant healing factors like collagen and blood vessel formation.
- The research identifies the mTOR signaling pathway as a key mechanism through which P311 enhances vascular endothelial growth factor (VEGF) production in MSCs, supporting the potential for P311-modified MSCs in more effective skin wound healing treatments.
Fenton reactions have been recently applied in tumor catalytic therapy, whose efficacy suffers from the unsatisfactory reaction kinetics of Fe to Fe conversion. Here we introduce a co-catalytic concept in tumor catalytic therapy by using a two-dimensional molybdenum disulfide (MoS ) nanosheet atomically dispersed with Fe species. The single-atom Fe species act as active sites for triggering Fenton reactions, while the abundant sulfur vacancies generated on the nanosheet favor electron capture by hydrogen peroxide for promoting hydroxyl radical production.
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- - This study explores using natural enzyme-like coordination geometry to create a new therapeutic approach for treating rheumatoid arthritis by designing a composite nanomedicine that mimics the activity of natural antioxidants.
- - The nanomedicine is developed by embedding cationic porphyrin ligands in a manganese-engineered silica carrier, which releases these components in mildly acidic environments, allowing for the synthesis of manganese porphyrin that exhibits enzymatic properties similar to superoxide dismutase (SOD) and catalase.
- - Experimental results show that this composite effectively reduces inflammation by promoting an anti-inflammatory shift in M1 macrophages and additionally supports bone regeneration by aiding biomineralization processes.
Article Synopsis
- Epidermal stem cells (EpSCs) are crucial for skin healing and maintenance, but their response to photodynamic therapy (PDT) needed further exploration in this study.
- Researchers used mouse models and 5-aminolevulinic acid (ALA) for PDT on wounds, finding that it sped up healing by promoting EpSC differentiation, proliferation, and migration.
- PDT not only enhanced wound closure and reduced inflammation but also increased the presence of growth factors, suggesting potential clinical applications for improving skin wound treatments.
Background: Delayed wound healing remains a common but challenging problem in patients with acute or chronic wound following accidental scald burn injury. However, the systematic and detailed evaluation of the scald burn injury, including second-degree deep scald (SDDS) and third-degree scald (TDS), is still unclear. The present study aims to analyze the wound-healing speed, the formation of granulation tissue, and the healing quality after cutaneous damage.
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- The corrosion of documents caused by iron gall ink poses a significant threat to Western cultural heritage due to the generation of reactive oxygen species from the iron gall complex.
- Researchers have adapted this pro-oxidative mechanism for use in cancer therapy by creating a nanomedicine that releases gallate and iron in acidic tumor environments.
- This novel approach produces a nanocomplex that generates hydrogen peroxide and hydroxyl radicals, leading to oxidative damage in tumors, potentially advancing future cancer treatment strategies.
Article Synopsis
- Ferroptosis and autophagy are two types of programmed cell death that are important in cancer therapy, but combining them effectively remains challenging.
- The study presents a novel nanomedicine, called TreMMM nanoparticles, that enhances ferroptosis while promoting autophagy, utilizing the release of trehalose and glutathione depletion.
- This combined approach shows significant antitumor effects in both lab tests and animal models, offering a promising strategy for more effective cancer treatments.
Article Synopsis
- The study investigated the effectiveness of autologous platelet-rich plasma (PRP) in promoting wound healing in mice, comparing it with a saline control.
- Results showed that PRP significantly enhanced wound healing by reducing inflammation, increasing new blood vessel formation (angiogenesis), and improving skin cell regeneration (re-epithelialization).
- The benefits of PRP were linked to decreased inflammatory cytokines and increased growth factors, suggesting its potential clinical application in treating wounds, although more research is needed to understand the mechanisms involved.
Article Synopsis
- The study focuses on the problem of restenosis after angioplasty, which results from endothelial injury and leads to excessive growth of vascular smooth muscle cells.
- Researchers aimed to explore whether exosomes from mesenchymal stem cells (MSC-Exo) could inhibit neointimal hyperplasia in a rat model following carotid artery injury, investigating their mechanisms.
- Results indicated that MSC-Exo were taken up by endothelial cells, reduced neointimal hyperplasia, and enhanced re-endothelialization, as shown by various staining and immunohistochemistry methods.
Article Synopsis
- * Most burn cases involved men (73.62%) and children under 10 years (29.57%), with higher admissions during the winter months due to various causes, notably fire (40.42%) and electricity (30.85%).
- * Key findings highlighted that deeper burns and larger affected areas increased the need for surgery and amputation, with the necessity for preventive measures targeting children and non-workplace incidents.
Article Synopsis
- Burn injuries disrupt the intestinal barrier, leading to inflammation and increased permeability, which triggers the release of pro-inflammatory cytokines like IL-17A and TNF-α.
- * Neutralizing IL-17A significantly reduces intestinal damage and inflammation, helping to preserve the barrier function and lower cytokine levels.
- * The study identifies IL-17AVγ4 T cells as a key source of IL-17A in the intestines following burn injury, underscoring the importance of targeting this pathway for potential therapeutic strategies.
Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management. In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner. The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.
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- Researchers aimed to create an ideal wound dressing combining good biocompatibility, a favorable porous structure, strong mechanical properties, and effective antibacterial action against drug-resistant bacteria.
- They developed a flexible dressing from a thermoplastic polyurethane (TPU) membrane, enhancing it with a polydopamine coating to incorporate nano-silver for antibacterial benefits while maintaining a suitable pore size and porosity.
- Testing showed the TPU/NS membranes had excellent antibacterial properties without harming mammalian cells, demonstrating promise for safe and effective clinical wound care.
Article Synopsis
- Predatory stress activates the HPA axis, impacting stress hormone levels and immune responses in mice, with a focus on differences between left-pawed and right-pawed mice.
- The study involved exposing mice to a cat, measuring their stress response through plasma hormones and gene expression analysis after acute and chronic exposure.
- Results showed that chronic stress significantly elevated stress hormones and altered gene expression, with notable differences in response between the left-pawed and right-pawed mice, suggesting lateralization impacts stress resilience.