The herbicidal activity and mechanism of Talaromyces purpureogenus CY-1 metabolites.

Background: Studies have shown that Talaromyces can produce a large number of secondary metabolites in its metabolic process, many of which have good insecticidal, antibacterial, antitumor, antiviral and other biological activities. In order to explore the herbicidal activity and mechanism of Talaromyces purpureogenus CY-1, we determined the inhibitory effect of the fermentation broth of the CY-1 strain on weeds, identified the major active components, and further investigated the herbicidal mechanism.

Result: The results showed that CY-1, with IC50 values of 5.

Quercetin and kaempferol from saffron petals alleviated hydrogen peroxide-induced oxidative damage in B16 cells.

Background: Saffron petals are usually considered as waste after saffron harvest. However, saffron petals contain many important phytochemical components (e.g.

Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells.

Glyphosate is a widely used broad-spectrum herbicide in agriculture and horticulture to control a variety of weeds and undesirable plants. However, the excessive use of glyphosate has raised a number of environmental and human health concerns. It is urgent to develop tools to detect glyphosate.

Purification and characteristics of a novel milk-clotting metalloprotease from Bacillus velezensis DB219.

Milk-clotting enzyme (MCE) is the essential active agents in dairy processing. The traditional MCE is mainly obtained from animal sources, in which calf rennet is the most widely used in cheese industry. Traditional MCE substitute is becoming necessary due to its limited production and increased cheese consumption.

Isolation and production optimization of a novel milk-clotting enzyme Bacillus velezensis DB219.

The milk-clotting enzyme (MCE) is a crucial ingredient in cheese manufacture. Due to the limits of traditional MCE, finding viable substitute is a pressing issue. This study aims to isolate and identify a wild strain with high milk-clotting activity (MCA) and low proteolytic activity (PA) and optimize the fermentation conditions for MCE production.

Preparation and characterization of flexible laminated composites impregnated with TPU/SiO for static puncture resistance.

At present, people face the danger of various occupational exposures, resulting in the rapid development of protective composites among which puncture-resistant materials are an essential component. In this article, composites impregnated with polyester polyurethane (TPU)/SiO are used to improve static puncture resistance. Different types of TPU and different concentrations of SiO in the impregnation solution are selected.

Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance.

A siderophore-based active bacterial pull-down strategy was integrated in a localized surface plasmon resonance (LSPR) sensing platform and subsequently tested by detecting whole-cell . The LSPR-based whole-cell sensing approach was previously demonstrated with aptamer-based molecular recognition motifs, and here it is extended to the powerful siderophore system, which exploits the natural bacterial need to sequester Fe(III). Specifically, a biscatecholate-monohydroxamate mixed ligand siderophore linked to a biotin three polyethylene glycol repeating units was synthesized and immobilized on Au trigonal nanoprisms of an LSPR sensor.

Nocardia panacis sp. nov., a novel actinomycete with antiphytopathogen activity isolated from the rhizosphere of Panax notoginseng.

Strain YIM PH21724 was isolated from the rhizosphere of Panax notoginseng. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain exhibits close phylogenetic relatedness to Nocardia kroppenstedtii N1286 (97.70%), Nocardia farcinica NCTC 11134 (97.

Enhanced sensitivity of capture IgE‑ELISA based on a recombinant Der f 1/2 fusion protein for the detection of IgE antibodies targeting house dust mite allergens.

The detection of allergen‑specific immunoglobulin (Ig)E is an important method for the diagnosis of IgE‑mediated allergic diseases. The sensitivity of the indirect IgE‑ELISA method against allergen extracts is limited by interference from high IgG titers and low quantities of effectual allergen components in extracts. To overcome these limitations, a novel capture IgE‑ELISA based on a recombinant Der f 1/Der f 2 fusion protein (rDer f 1/2) was developed to enhance the sensitivity to IgEs that bind allergens from the house dust mite (HDM) species Dermatophagoides farina.

Amycolatopsis panacis sp. nov., isolated from Panax notoginseng rhizospheric soil.

A novel Gram-positive bacterium, designated strain YIM PH21725, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0.

Microscale and Nanoscale Electrophotonic Diagnostic Devices.

Detecting and identifying infectious agents and potential pathogens in complex environments and characterizing their mode of action is a critical need. Traditional diagnostics have targeted a single characteristic (e.g.

Anti-IgE therapy for IgE-mediated allergic diseases: from neutralizing IgE antibodies to eliminating IgE B cells.

Allergic diseases are inflammatory disorders that involve many types of cells and factors, including allergens, immunoglobulin (Ig)E, mast cells, basophils, cytokines and soluble mediators. Among them, IgE plays a vital role in the development of acute allergic reactions and chronic inflammatory allergic diseases, making its control particularly important in the treatment of IgE-mediated allergic diseases. This review provides an overview of the current state of IgE targeted therapy development, focusing on three areas of translational research: IgE neutralization in blood; IgE-effector cell elimination; and IgE B cell reduction.

Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance Aptasensor.

The detection of whole-cell Pseudomonas aeruginosa presents an intriguing challenge with direct applications in health care and the prevention of nosocomial infection. To address this problem, a localized surface plasmon resonance (LSPR) based sensing platform was developed to detect whole-cell Pseudomonas aeruginosa strain PAO1 using a surface-confined aptamer as an affinity reagent. Nanosphere lithography (NSL) was used to fabricate a sensor surface containing a hexagonal array of Au nanotriangles.

Optical Biosensing of Bacteria and Bacterial Communities.

Bacterial sensing is important for understanding the numerous roles bacteria play in nature and in technology, understanding and managing bacterial populations, detecting pathogenic bacterial infections, and preventing the outbreak of illness. Current analytical challenges in bacterial sensing center on the dilemma of rapidly acquiring quantitative information about bacteria with high detection efficiency, sensitivity, and specificity, while operating within a reasonable budget and optimizing the use of ancillary tools, such as multivariate statistics. This review starts from a general description of bacterial sensing methods and challenges, and then focuses on bacterial characterization using optical methods including Raman spectroscopy and imaging, infrared spectroscopy, fluorescence spectroscopy and imaging, and plasmonics, including both extended and localized surface plasmon resonance spectroscopy.

Polymer particles filled with multiple colloidal silica via in situ sol-gel process and their thermal property.

The in situ formation of dielectric silica (SiO) particles was carried out in the presence of temperature-responsive poly(N-isopropylacrylamide) particles. Unlike the typical sol-gel method used to prepare various SiO particles, the highly uniform growth of SiO particles was achieved within the cross-linked polymer particles (i.e.

Pains and Gains from China's Experiences with Emerging Epidemics: From SARS to H7N9.

Over the recent decades, China experienced several emerging virus outbreaks including those caused by the severe acute respiratory syndrome- (SARS-) coronavirus (Cov), H5N1 virus, and H7N9 virus. The SARS tragedy revealed faults in China's infectious disease prevention system, propelling the Chinese government to enact reforms that enabled better combating of the subsequent H1N1 and H7N9 avian flu epidemics. The system is buttressed by three fundamental, mutually reinforcing components: (1) enduring government administration reforms, including legislation establishing a unified public health emergency management system; (2) prioritized funding for biotechnology and biomedicine industrialization, especially in the areas of pathogen identification, drug production, and the development of vaccines and diagnostics; and (3) increasing investment for public health and establishment of a rapid-response infectious diseases prevention and control system.

Dissociation between Conceptual and Perceptual Implicit Memory: Evidence from Patients with Frontal and Occipital Lobe Lesions.

The latest neuroimaging studies about implicit memory (IM) have revealed that different IM types may be processed by different parts of the brain. However, studies have rarely examined what subtypes of IM processes are affected in patients with various brain injuries. Twenty patients with frontal lobe injury, 25 patients with occipital lobe injury, and 29 healthy controls (HC) were recruited for the study.

Explicit memory and implicit memory in occipital lobe stroke patients.

Background: Occipital stroke patients mainly showed cortical blindness and unilateral vision loss; memory is generally reserved. Recent reports from neuroimaging show the occipital lobe may be involved in the processing of implicit memory (IM), especially the perception type of IM processing. In this study, we explored the explicit memory (EM) and IM damage in occipital lobe stroke patients.

Sunlight-induced synthesis of various gold nanoparticles and their heterogeneous catalytic properties on a paper-based substrate.

This work describes the light-induced preparation of various gold nanoparticles and demonstrates their possible use as efficient photothermal heating materials and practical heterogeneous catalysts under the irradiation of a solar-based light after being loaded onto a paper-based substrate. The synthesis of gold nanoparticles was accomplished under the irradiation of daily sunlight and a solar-simulated light with an intensity that was closely adjusted to the one-sun condition. Tunable sizes of gold nanoparticles were systematically controlled by the ratio of trisodium citrate and gold chloride ions, particularly with the solar-simulated light source.

Thermally tunable catalytic and optical properties of gold-hydrogel nanocomposites.

We have developed a very simple approach for preparing physically embedded gold cores in a temperature-responsive hydrogel polymer nanoparticle under fluorescent light irradiation. The complete encapsulation of the multiple gold core nanoparticles is confirmed by the catalytic reduction of 4-nitrophenol, whose reactivity is significantly retarded above the lower critical solution temperature (LSCT) due to the deswelled polymer structure; its increased hydrophobicity slows the access of hydrophilic reactants to the cores. Since these gold cores are physically embedded in the polymer nanoparticles, further growth of the cores is reliably achieved in situ under light irradiation.

[Determination of tenacissoside H in Marsdeniae tenacissimae by HPLC-ELSD].

In order to improve the quality standard of Marsdenia tenacissima, a quantitative determination method of tenacissoside H was developed using high performance liquid chromatography. The method was carried out on a YMC ODS-H80 (4.6 mm x 250 mm, 4 microm) column eluted with a mixture of acetonitrile and water (50:50) as the mobile phase.