Evaluation of an Ussing Chamber System Equipped with Rat Intestinal Tissues to Predict Intestinal Absorption and Metabolism in Humans.

Background And Objective: Oral bioavailability (F) is one of the key factors that need to be determined in drug discovery. This factor is determined by the permeability and solubility of new molecule entities (NMEs) according to the biopharmaceutics classification system (BCS).

Methods: In the present study, we evaluated the permeability of 22 drugs in rat intestinal tissues using an Ussing chamber system and correlated the permeability with data on human intestinal absorption (F) and intestinal availability (F × F) reported in the literature.

RNA sensor MDA5 suppresses LINE-1 retrotransposition by regulating the promoter activity of LINE-1 5'-UTR.

Background: Type 1 long interspersed elements, or LINE-1, are the only retroelements that replicate autonomously in human cells. The retrotransposition process of LINE-1 can trigger the activation of the innate immune system and has been proposed to play a role in the development of several autoimmune diseases, including Aicardi-Goutières syndrome (AGS). In contrast, all known AGS-associated proteins, except MDA5, have been reported to affect LINE-1 activity.

MicroRNA-17 as a promising diagnostic biomarker of gastric cancer: An investigation combining TCGA, GEO, meta-analysis, and bioinformatics.

Integrated studies of accumulated data can be performed to obtain more reliable information and more feasible measures for investigating potential diagnostic biomarkers of gastric cancer (GC) and to explore related molecular mechanisms. This study aimed to identify microRNAs involved in GC by integrating data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus. Through our analysis, we identified hsa-miR-17 (miR-17) as a suitable candidate.

Clinical observation of antibiotics in preventing nosocomial infection in premature infants.

Objective: To study the possibility of lower the nosocomial infection rate among the premature infants by prophylactic use of antibiotics.

Methods: Retrospectively collect 409 premature infants admitted to the neonatal intensive care unit (NICU) of First Hospital of Jilin University between 1st January in 2009 and 31th December in 2011, who meet inclusion criteria. There were prohylactic group and nonprohylactic group randomly divided and compared the nosocomial infection rates of two groups.