Ammonium chloride mitigates the amplification of fish virus by blocking autophagy-dependent replication.

Ammonia fertilizer, primarily composed of ammonium chloride, is widely used in pond fish farming throughout Asia. Despite the belief that it possesses antiviral properties, the underlying mechanisms remain unclear. Ammonium chloride (NH4Cl) has been demonstrated to act as a potent inhibitor of autophagy, which is used by many fish viruses to promote their proliferation during infection.

Intrapancreatic adipocytes and beta cell dedifferentiation in human type 2 diabetes.

Aims/hypothesis: Fat deposition in the pancreas is implicated in beta cell dysfunction and the progress of type 2 diabetes. However, there is limited evidence to confirm the correlation and explore how pancreatic fat links with beta cell dysfunction in human type 2 diabetes. This study aimed to examine the spatial relationship between pancreatic fat and islets in human pancreases.

Optimized Porous KTi(PO)@N-Doped Carbon Electrode for Enhanced Sodium/Potassium-Storage and Accelerated Activation Process.

Porous KTi(PO) nanoparticles are synthesized via a solvothermal method and subsequently modified with nitrogen-doped carbon layers by using polydopamine as the carbon source. The resultant KTi(PO)@N-doped carbon composite (KTP@NC) exhibits a preserved porous structure with abundant pores, facilitating ion diffusion and electrolyte infiltration. Various characterizations, including X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy, reveal the successful formation of an interconnected nitrogen-doped carbon network.

Highly Stable, Excellent Foliar Adhesion and Anti-Photodegradation Nucleic Acid-Peptide Coacervates for Broad Agrochemicals Delivery.

Agrochemicals play a pivotal role in the management of pests and diseases and the way agrochemicals are utilized exerts significant impacts on the environment. Ensuring rational application and improving utilization rates of agrochemicals are major demands in developing green delivery systems. Herein, a model of nucleic acid-peptide coacervate (NPC) for agrochemical delivery is presented, which is formed by mixing negatively charged single-stranded DNAs with positively charged poly-L-lysine.

Isolated from Populus euphratica rhizosphere soil, and mining their metabolites.

Introduction: The microbial community in desert ecosystems is a vital and highly active component. , one of the dominant genera within this community, exhibits significant capabilities in metabolic degradation and the synthesis of secondary metabolites.

Methods: To investigate the metabolic potential of , two strains of were isolated from rhizosphere soil samples of in the Taklimakan Desert during the initial phase of this study, TRM 70351 and TRM 70361.

Functionalized zeolite regulates bone metabolic microenvironment.

The regulation of bone metabolic microenvironment imbalances in diseases such as osteoporosis, bone defects, infections, and tumors remains a significant challenge in orthopedics. Therefore, it has become urgent to develop biomaterials with effective bone metabolic microenvironmental regulatory functions. Zeolites, as advanced biomedical materials, possess distinctive physicochemical properties such as multi-level pore structures, adjustable frameworks, easily modifiable surfaces, and excellent adsorption capabilities.

Differences in storage stability of cow's milk-based and goat's milk-based infant formulas.

Goat's milk-based infant formula (YIF) has the advantages of high nutritional value and hypoallergenicity, and fewer studies have been conducted on YIF. Therefore, this study examined the changes in physicochemical and functional properties of cow's milk-based infant formula (ZIF) and YIF during 6 months of storage at 25 °C, 37 °C, and 50 °C, respectively. The results showed that YIF had higher pH (7.

Enhancing Session-Based Recommendation With Multi-Interest Hyperbolic Representation Networks.

Session-based recommendation (SBR) aims to predict the next item a user might click within an ongoing session, without relying on user profiles or historical data. Modern approaches typically use graph networks to learn item embeddings in Euclidean space via graph convolution operations. However, they often struggle to capture the diversity of user interactions within short, hierarchically structured sessions, which is essential for accurate predictions in SBR.

CT-Less Whole-Body Bone Segmentation of PET Images Using a Multimodal Deep Learning Network.

In bone cancer imaging, positron emission tomography (PET) is ideal for the diagnosis and staging of bone cancers due to its high sensitivity to malignant tumors. The diagnosis of bone cancer requires tumor analysis and localization, where accurate and automated wholebody bone segmentation (WBBS) is often needed. Current WBBS for PET imaging is based on paired Computed Tomography (CT) images.

Injectable biodegradable microchamber array films for long-term delivery of glucocorticoids.

Glucocorticoids (GCs) are widely recognized for their potent anti-inflammatory and analgesic effects. Although they can cause an array of side effects when delivered systemically these are generally avoided when delivered locally at disease sites such as the eyes, lungs and joints. Glucocorticoid formulations for local use range from crystals and particles through to non-biodegradable implants.

Nanoconfined in-situ sampling and extracting switching needle device construction for highly enrichment of polar metabolites in human urine.

Urine, as a non-invasive sampling specimen, offers significant advantages for the diagnosis and treatment of diseases. However, as a water-based matrix, most metabolites in urine are high polarity, which limits the ultrafast extraction and high enrichment of these polar metabolites from urine. Though solvent-based extraction method has demonstrated considerable benefits in extraction rates, the necessity for selecting water-immiscible solvents restricts the extraction of highly polar metabolites.

Association of Sleep Traits, Physical Activity, and Sedentary Leisure Behavior With Female Reproductive Health: A Two-Sample Mendelian Randomization Analysis.

Purpose: Living habits affect female endocrine function and fertility, along with aging and other life factors. Because of this, female reproductive health can be promoted through living habit adjustment.

Methods: We used two-sample Mendelian randomization analysis with summary datasets from a genome-wide association study to investigate the causal relationships between sleep traits, sedentary leisure behavior, physical activity, and reproductive health traits in women, calculating odds ratios (ORs) and 95% confidence intervals (CIs).

Ferroptosis emerges as the predominant form of regulated cell death in goat sperm cryopreservation.

Background: Freezing-induced sperm damage, often associated with oxidative stress, can result in regulated cell death. Given that oxidative stress can trigger various forms of regulated cell death, the prevailing form during sperm cryopreservation remains unknown. Our study aimed to investigate this issue using cashmere goats as a model.

Chemotaxis-driven hybrid liposomes recover intestinal homeostasis for targeted colitis therapy.

Inflammatory bowel disease (IBD) is closely linked to the dysregulation of intestinal homeostasis, accompanied by intestinal epithelial barrier destruction, dysbiosis of gut microbiota, subsequent inflammatory factor infiltration, and excessive oxidative stress. Conventional therapeutics focus on suppressing inflammation and often suffer from metabolic instability as well as limited targeting, thereby leading to suboptimal remission rates and severe side effects. Here, we designed bacterial outer membrane vesicle (OMV, from Stenotrophomonas maltophilia)-fused and borneol-modified liposomes (BO/OMV-lipo@LU) for targeted delivery of luteolin to recover intestinal homeostasis by alleviating inflammation and modulating dysregulated intestinal epithelial barrier, redox balance, and gut microbiota in IBD.

Ecological stoichiometry in leaves, branches, and soils of at different stand ages in the Taihang Mountains, China.

To understand the changes and relationship of plant-soil eco-stoichiometric characteristics of plantations with stand ages, we investigated plantations of different stand ages (10, 23, 39, and 47 years old) in the Taihang Mountains. We measured the concentration and stoichiometry of carbon (C), nitrogen (N), and phosphorus (P) in leaves, branches, and soils at different layers. We analyzed the relationship between leaf, branch nutrient characteristics and soil physicochemical properties.

An integrated optimization strategy by Joule heating technique enabling rapid fabrication of robust LiAlTi(PO) solid-state electrolyte for all-solid-state lithium metal batteries.

The advancement of high-energy-density and intrinsically safe all-solid-state lithium metal batteries (ASSLMBs) is crucial for the comprehensive electrification of energy consumption. Nevertheless, intrinsic defects in solid-state electrolytes (SSEs) and suboptimal interfacial contacts often lead to uncontrolled dendritic growth during practical battery operation. To address these challenges, this study proposes an integrated optimization strategy for bulk-phase defects and interfacial contacts of LiAlTi(PO) (LATP) SSEs based on ultra-fast Joule heating sintering (UHS) technique.

Oocyte transcriptomes and follicular fluid proteomics of ovine atretic follicles reveal the underlying mechanisms of oocyte degeneration.

Background: In mammals, female fertility is influenced by the result of follicular development (ovulation or atresia). Follicular atresia is a complex physiological process that results in the degeneration of oocytes from the ovary. However, the molecular mechanisms of oocyte degeneration and key protein markers of follicular atresia remain unclear.

Forensic identification of fatal mushroom poisoning: a case report.

Mushroom poisoning incidents happen infrequently, yet owing to the non-lethal nature of most toxins and the efficacy of timely treatment, fatalities from mushroom poisoning are uncommon, leading to a scarcity of pertinent clinical and pathological data. Here, we reported a case of death caused by the consumption of raw mushrooms, alongside detailed clinical data and multi-organs pathological alterations, which underscored its potential significant reference value in forensic practice. Futhermore, ibotenic acid, a type of mushroom toxin, was detected both in the patient's blood and gastric lavage fluid about 19 h after the consumption of mushrooms, and was successfully quantified at concentrations of 0.

Smurf2 Suppresses Proliferation and Cell Cycle of Triple-Negative Breast Cancer Cells by Promoting the Polyubiquitination and Degradation of RPL35A.

Human L35a ribosomal protein (RPL35A) has been reported to confer higher drug resistance and viability to triple-negative breast cancer (TNBC) cells, but the mechanism related to its promotion of TNBC malignant progression is still unclear. Here, we found that silencing of RPL35A could inhibit the proliferation of TNBC cells by suppressing the G1/S phase transition. Furthermore, SMAD-specific E3 ubiquitin protein ligase 2 (Smurf2) was found to be a potential upstream ubiquitin ligase of RPL35A.

A Spiropyran-Based Hydrogel Composite for Wearable Detectors to Monitor Visible Light Intensity to Prevent Myopia.

A wearable detector to monitor visible light intensity is realized by the restrained photochromism of a hydrogel composite containing light-responsive spiropyran with hydroxyl groups (SPOH). When exposed to visible light, the SPOH experiences a ring-opening to a ring-closed transition accompanied by discoloration from red to yellow. Unlike in the solution, the photochromism/discoloration rate is strongly correlated to the cross-linking points.

Ribes diacanthum Pall modulates bile acid homeostasis and oxidative stress in cholestatic mice by activating the SIRT1/FXR and Keap1/Nrf2 signaling pathways.

Ethnopharmacological Relevance: Cholestatic liver injury (CLI) is a pathophysiological syndrome characterized by the accumulation of bile acids (BAs), which leads to significant hepatic dysfunction. This condition is frequently associated with disturbances in BAs homeostasis and the induction of oxidative stress. Ribes diacanthum Pall (RDP), a conventional folk medicinal plant, has been employed in Mongolia, the Inner Mongolia region of China, and other areas for the remediation of hepatic disorders.

Cyclooxygenase 2 overexpression suppresses Smad3 and augments ERK1/2 signaling activated by TGFβ1 in endometrial stromal cells: A novel insight into endometriosis pathogenesis.

Research Question: To investigate the underlying mechanisms driving the opposing effects of transforming growth factor-beta 1 (TGFβ1) on the proliferation of control (CESCs) and ectopic (EESCs) endometrial stromal cells.

Design: Cell proliferation assays (CCK-8 and colony formation) were employed to assess the effects of TGFβ1 on CESC and EESC proliferation. An immortalized human endometrial stromal cell line (HESC) was used to elucidate the mechanisms behind cytostatic effect of TGFβ1 and the potential role of cyclooxygenase (COX)-2 in mediating the modulation of TGFβ1 signaling.

Toxicity of Per- and Polyfluoroalkyl Substances and Their Substitutes to Terrestrial and Aquatic Invertebrates-A Review.

Per- and polyfluoroalkyl substances (PFASs) have been widely used in daily life but they cause certain impacts on the environment due to their unique carbon-fluorine chemical bonds that are difficult to degrade in the environment. Toxicological studies on PFASs and their alternatives have mainly focused on vertebrates, while terrestrial and aquatic invertebrates have been studied to a lesser extent. As invertebrates at the bottom of the food chain play a crucial role in the whole ecological chain, it is necessary to investigate the toxicity of PFASs to invertebrates.