MarR family regulator LcbR2 activates lincomycin biosynthesis in multiple ways.

Lincomycin, produced by the actinomycete Streptomyces lincolnensis, is highly effective against Gram-positive bacteria and protozoans, making it widely used in clinical settings. This study identified LcbR2, a MarR family transcriptional regulator, as an activator of lincomycin biosynthesis. Knocking out the lcbR2 gene reduced lincomycin production by 63.

Highly efficient and convenient QuEChERS using ZIF-67 derived magnetic nanoporous carbon for determination of carbamate pesticides in various vegetable and fruit samples.

Effective and convenient QuEChERS of lipophilic pesticides with wide pK range from strongly pigment-rich food samples remains a great challenge. Here, a ZIF-67 derived magnetic nanoporous carbon (Co@MPC) was firstly proposed for modified QuEChERS of carbamate pesticides (pK 4.3-12.

Exploring microfluidics-based organoid interactions through analysis of albumin secretion.

Organoids-on-a-chip exhibit significant potential for advancing disease modeling, drug screening, and precision medicine, largely due to their capacity to facilitate interactions among organoids. However, the influence of chip design on these interactions remains poorly understood, primarily due to our limited knowledge of the mediators of communication and the complexity of interaction dynamics. This study demonstrates that analyzing albumin secretion from liver organoids within an organoids-on-a-chip system can provide a measure of the interaction intensity among organoids, offering valuable insights into how chip design influences these interactions.

Efficient Photocatalytic Propane Direct Dehydrogenation to Propylene Over PtO Clusters.

The direct dehydrogenation of alkanes to olefins under mild conditions is challenging due to the inert nature of alkyl C─H bonds. Herein, an efficient photocatalytic system is developed for propane direct dehydrogenation (PDH) to propylene, consisting of ≈1.30 nm sized PtO clusters immobilized on a layered double hydroxide -derived ZnO/AlO support (LD-Pt).

Survival and prognostic factors among different types of liposarcomas based on SEER database.

The aim of this study is to elucidate the disparities in survival and risk factors among different subtypes of liposarcoma, through analysis of epidemiological and prognostic data. The study cohort consisted of 12,822 patients diagnosed with liposarcoma in the United States between 2000 and 2021, whose data were retrieved from the Surveillance, Epidemiology, and End Results (SEER) program. The prognosis for different subtypes of liposarcoma and the associated factors such as age, tumor stage, intervention, gender, tumor grade, location, size, chemotherapy and radiotherapy, were retrieved from the database.

Xenia Effect on Nutritional and Flavor Components of 'Jingbaili' Pear.

The 'Jingbaili' pear is a national geographical indication product of China, featuring an oblate shape and being rich in nutrients. But the quality of the 'Jingbaili' pear is unstable. Xenia can cause changes in the quality of pears, but the effect of xenia on the 'Jingbaili' pear is unknown, and its mechanism is still unclear.

N skeleton-regulated cobalt phthalocyanine promotes polysulfide adsorption and redox kinetics.

An N skeleton substituent on cobalt phthalocyanine (CoPc) was meticulously studied to redistribute the charge in phthalocyanine, improve the mass diffusion, and promote the redox kinetics of polysulfides (LiPS), resulting in a significant ultra-low capacity decay of 0.11% at 5C over 500 cycles.

Li-Based Nanoprobes with Boosted Photoluminescence for Temperature Visualization in NIR Imaging-Guided Drug Release.

Lanthanide-doped fluoride nanocrystals have emerged as promising tools in biomedicine, yet their applications are still limited by their low luminescence efficiency. Herein, we developed highly efficient lithium-based core-shell-shell (CSS) nanoprobes (NPs) featuring a rhombic active domain and a spherical inert protective shell. By introducing Yb as an energy transfer bridge and optimizing the CSS design, a remarkable 1643-fold enhancement in visible emission and a 33-fold increase in NIR emission are achieved compared to original nanoparticles.

Sea buckthorn polyphenols on gastrointestinal health and the interactions with gut microbiota.

The potential health benefits of sea buckthorn polyphenols (SBP) have been extensively studied, attracting increasing attention from researchers. This paper reviews the composition of SBP, the effects of processing on SBP, its interactions with nutrients, and its protective role in the gastrointestinal tract. Polyphenols influence nutrient absorption and metabolism by regulating the intestinal flora, thereby enhancing bioavailability, protecting the gastrointestinal tract, and altering nutrient structures.

Clinical Outcome of Extended Curettage with Postoperative Denosumab Administration for the Treatment of Campanacci Grade III Giant Cell Tumors of the Extremities.

Purpose: To investigate the local recurrence rate, joint preservation status, and functional outcomes after extended curettage and postoperative denosumab treatment for Campanacci Grade III giant cell tumors of the extremities.

Methods: We retrospectively reviewed 23 patients with Campanacci Grade III GCTB of the extremities in our hospital between January 2017 and June 2023 who underwent extended curettage and postoperative denosumab administration alone, without preoperative denosumab treatment. Patients were followed-up for adverse events of denosumab, surgical outcomes, limb function of lesions, and local recurrence following extended curettage with postoperative denosumab.

Time-frequency-space transformer EEG decoding for spinal cord injury.

Transformer neural networks based on multi-head self-attention are effective in several fields. To capture brain activity on electroencephalographic (EEG) signals and construct an effective pattern recognition model, this paper explores the multi-channel deep feature decoding method utilizing the self-attention mechanism. By integrating inter-channel features with intra-channel features, the self-attention mechanism generates a deep feature vector that encompasses information from all brain activities.

Chlorella-Based Biohybrid Microrobot for Removing Both Nutrient and Microalgae toward Efficient Water Eutrophication Treatment.

Excessive nutrients and explosive growth of harmful microalgae in water environments are key challenges in the treatment of eutrophication. The development of a low-cost, time-saving, and small-space-suitable research method that can simultaneously remove nutrients and microalgae is highly anticipated. This work first proposed applying microrobots to eutrophication treatment.

Discovery and optimization of tetrahydroacridine derivatives as a novel class of antibiotics against multidrug-resistant Gram-positive pathogens by targeting type I signal peptidase and disrupting bacterial membrane.

Increasing antimicrobial resistance underscores the urgent need for new antibiotics with unique mechanisms. Type I signal peptidase (SPase I) is crucial for bacterial survival and a promising target for antibiotics. Herein we designed and synthesized innovative tetrahydroacridine-9-carboxylic acid derivatives by optimizing the initial hit compound SP11 based on virtual screening.

Quantification of intratumoral heterogeneity using habitat-based MRI radiomics to identify HER2-positive, -low and -zero breast cancers: a multicenter study.

Background: Human epidermal growth factor receptor 2-targeted (HER2) therapy with antibody-drug conjugates has proven effective for patients with HER2-low breast cancer. However, intratumoral heterogeneity (ITH) poses a great challenge in identifying HER2-low tumors. ITH signatures were developed by quantifying ITH to differentiate HER2-positive, -low and -zero breast cancers.

Functional connectivity of EEG motor rhythms after spinal cord injury.

Spinal cord injury (SCI), which is the injury of the spinal cord site resulting in motor dysfunction, has prompted the use of motor imagery (MI)-based brain computer interface (BCI) systems for motor function reconstruction. However, analyzing electroencephalogram signals and brain function mechanisms for SCI patients is challenging. This is due to their low signal-to-noise ratio and high variability.

Antioxidative hydrogel based on hyaluronic acid/polyethylene glycol loaded with shikonin-coated flexible liposome and evaluation of its therapeutic effect on eczema.

Eczema, a common skin condition, is gaining attention due to its increasing annual prevalence and severe influence on both bodily and mental health. Existing treatments for this disease often have significant side effects and safety concerns. Comfrey, a traditional Chinese herbal remedy, is commonly used to treat skin conditions.

Participates in the Root Secretion of Phenolic Acids and Modulates the Development of Plant Replant Disease.

Phenolic allelochemicals from root exudates dominate rhizosphere formation, lead to autotoxicity in plants subjected to continuous monoculture (CM) stress and induce the emergence of replant disease. However, the regulatory mechanisms governing the transport of phenolics from plant roots to the rhizosphere remain poorly understood. A potential phenolic efflux transporter from , designated RgMATE35, has been preliminarily characterized.

Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1.

Diphthamide (DPH) is a highly conserved post-translational modification exclusively present in eukaryotic translation elongation factor 2 (eEF2), with its loss leading to embryonic lethality in mice and developmental disorders in humans. In this study, we unveil the role of diphthamide in mammalian cell DNA damage stress, with a particular emphasis on DNA replication stress. We developed a systematic strategy to identify human proteins affected by diphthamide with a combination of computational profiling and quantitative proteomics.