Effect of different restorative design and materials on stress distribution in cracked teeth: a finite element analysis study.

Objectives: To compare the stress distribution and crack propagation in cracked mandibular first molar restored with onlay, overlay, and two types of occlusal veneers using two different CAD/CAM materials by Finite Element Analysis (FEA).

Materials And Methods: A mandibular first molar was digitized using a micro CT scanning system in 2023. Three-dimensional dynamic scan data were transformed, and a 3D model of a cracked tooth was generated.

Vaccination with a DNA vaccine cocktail encoding TgROP2, TgROP5, TgROP9, TgROP16, TgROP17, and TgROP18 confers limited protection against Toxoplasma gondii in BALB/c mice.

Toxoplasmosis is a foodborne zoonotic parasitic disease caused by Toxoplasma gondii, which seriously threatens to human health and causes economic losses. At present, there is no effective vaccine strategy for the prevention and control of toxoplasmosis. T.

Exploring the complexity of xylitol production in the fungal cell factory Aspergillus niger.

Production of xylitol from agricultural by-products offers a promising approach for the circular bioeconomy. This study investigates the roles of transcription factors XlnR and CreA in xylitol production from wheat bran in Aspergillus niger by generating strains with a constitutively active XlnR (XlnR, V756F mutation) and/or deletion of creA, in a previously generated xylitol-producing strain. The XlnR mutation increased the initial rate of xylitol production but lowered the overall accumulation.

Surface hydrophobicity mechanism of poultry red mite, Dermanyssus gallinae (Acari: Dermanyssidae), gives novel meaning to chemical control.

Surface hydrophobicity of organisms provides biological self-protection. The hydrophobicity of pest surface, acting as a main obstacle for the pest control, can lead to low utilization and high loss of pesticides. Dermanyssus gallinae is a notorious pest in egg-laying hens, whose control primarily depends on acaricide spraying, while its surface hydrophobicity and potential influence on pesticide effectiveness are not clear.

Platelet and Erythrocyte Membranes Coassembled Biomimetic Nanoparticles for Heart Failure Treatment.

Cardiac fibrosis is a prevalent pathological process observed in the progression of numerous cardiovascular diseases and is associated with an increased risk of sudden cardiac death. Although the BRD4 inhibitor JQ1 has powerful antifibrosis properties, its clinical application is extremely limited due to its side effects. There remains an unmet need for effective, safe, and low-cost treatments.

Effect of Reinforcement Bar on Trueness of Printed Titanium Kennedy I Removable Partial Denture Frameworks by Selective Laser Melting.

Objective: The addition of reinforcement bars is a commonly used method to improve the fabrication trueness of selective laser melting removable partial denture alloy frameworks. However, the effects of different reinforcement bar designs on the trueness of the entire framework remain unclear. This study investigated the trueness of removable partial denture frameworks of pure titanium fabricated by selective laser melting under different reinforcement bar settings.

Influence of 3D printed surface micro-structures on molding performance and dental bonding properties of zirconia.

Objectives: To investigate the influence of the 3D printed micro-structured surfaces on the bond strength of zirconia to resin cement.

Methods: Zirconia specimens were divided into five groups based on manufacturing technique and surface preparation: (1) milled zirconia (M group); (2) milled zirconia airborne abraded (MA group); (3) printed zirconia (M group); (4) printed zirconia airborne abraded (PA group); and (5) printed zirconia with micro-structured surface (PM group). The surface morphology, cross-sectional morphology, and elemental composition were observed using a scanning electron microscope (SEM).

Influence of intraoral scanning duration on data accuracy.

Statement Of Problem: The use of intraoral scanners (IOSs) correlates with clinical outcome and patient satisfaction. While the accuracy of IOSs has been well evaluated, studies on the effect of scanning duration on data accuracy are limited.

Purpose: The purpose of this in vitro study was to investigate the relationship between different scanning durations and the accuracy of the scanned data.

Irisin Ameliorated Skeletal Muscle Atrophy by Inhibiting Fatty Acid Oxidation and Pyroptosis Induced by Palmitic Acid in Chronic Kidney Disease.

Introduction: Protein-energy waste (PEW) is a common complication in patients with chronic kidney disease (CKD), among which skeletal muscle atrophy is one of the most important clinical features of PEW. Pyroptosis is a type of proinflammatory, programmed cell death associated with skeletal muscle disease. Irisin, as a novel myokine, has attracted extensive attention for its protective role in the complications associated with CKD, but its role in muscle atrophy in CKD is unclear.

Mechanism of Antidepressant Action of (2R,6R)-6-Hydroxynorketamine (HNK) and Its Compounds: Insights from Proteomic Analysis.

The effects of HNK, I5, and I6 on the expression of protein in hippocampus of depressed mice were studied by isobaric tags for relative and absolute quantitation (iTRAQ) to explore the mechanism of their antidepressant action. HNK, I5, and I6 were administered intragastric administration once a day in the morning for 7 days. The drug was subsequently discontinued for 7 days (without any treatment).

Function of glycogen synthase kinase3 in embryogenesis of Dermanyssus gallinae.

In the life cycle of Dermanyssus gallinae, the embryo is a developmental stage that does not require blood meals, but needs glucose to produce adenosine triphosphate (ATP) through glycolysis or oxidative phosphorylation, providing energy for embryonic development. Glycogen synthase kinase 3 (GSK3), belonging to the serine/threonine kinase family, is a key enzyme involved in glycogen metabolism in many eukaryotes, but not be described in D. gallinae.

Risk factors for acute kidney injury in COVID-19 patients: an updated systematic review and meta-analysis.

Acute kidney injury (AKI) is associated with increased mortality among coronavirus disease 2019 (COVID-19) patients. This meta-analysis aimed to identify risk factors for the development of AKI in patients with COVID-19. A systematic literature search was conducted in PubMed and EMBASE from 1 December 2019 to 1 January 2023.

Identification of an l-Arabitol Transporter from .

l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in . Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR.

Glucometer Readout for Portable Detection of Heterogeneous Circulating Tumor Cells in Lung Cancer Captured on a Dual Aptamer Functionalized Wrinkled Cellulose Hydrogel Interface.

The rarity of circulating tumor cells (CTCs) poses a great challenge to their clinical application as reliable "liquid biopsy" markers for cancer diagnosis. Meanwhile, the epithelial-mesenchymal transition (EMT) led to a reduced efficiency in capturing cells with lost or downregulated epithelial cell adhesion molecule (EpCAM) expressions. In this study, we proposed an integrated, highly efficient strategy for heterogeneous CTC capture and portable detection from the blood of non-small-cell lung cancer (NSCLC) patients.

The Sugar Metabolic Model of Can Only Be Reliably Transferred to Fungi of Its Phylum.

Fungi play a critical role in the global carbon cycle by degrading plant polysaccharides to small sugars and metabolizing them as carbon and energy sources. We mapped the well-established sugar metabolic network of to five taxonomically distant species (, , , and ) using an orthology-based approach. The diversity of sugar metabolism correlates well with the taxonomic distance of the fungi.

Identification of guanine and hematin as arrestment pheromones of poultry red mites, Dermanyssus gallinae (Acari: Dermanyssidae) and their application in mite control.

Dermanyssus gallinae, also known as the poultry red mite (PRM), is one of widespread ectoparasite in the poultry industry worldwide, causing direct and indirect detriments to poultry as well as substantial financial losses. Novel control methods are urgently needed to improve the current acaricide-based control of D. gallinae.

GalR, GalX and AraR co-regulate d-galactose and l-arabinose utilization in Aspergillus nidulans.

Filamentous fungi produce a wide variety of enzymes in order to efficiently degrade plant cell wall polysaccharides. The production of these enzymes is controlled by transcriptional regulators, which also control the catabolic pathways that convert the released monosaccharides. Two transcriptional regulators, GalX and GalR, control d-galactose utilization in the model filamentous fungus Aspergillus nidulans, while the arabinanolytic regulator AraR regulates l-arabinose catabolism.

Study on the expression of TOP2A in hepatocellular carcinoma and its relationship with patient prognosis.

Background: Hepatocellular carcinoma (HCC) is a primary liver cancer with a high mortality rate. However, the molecular mechanism of HCC formation remains to be explored and studied.

Objective: To investigate the expression of TOP2A in hepatocellular carcinoma (HCC) and its prognosis.

Case Report: A Case of Meningoencephalitis in an Immunocompetent Patient After Invasive Operation.

As an opportunistic phytopathogen, has only been shown to cause neurological disease in immunocompromised patients, where antifungal therapy was not effective. We report a case of meningoencephalitis in an apparently immunocompetent young woman who presented with severe headache and slight fever after undergoing transnasal endoscopic repair of cerebrospinal fluid rhinorrhea. Chronic sinusitis and suspicious intracranial fungal lesions were observed on enhanced magnetic resonance imaging (MRI).

Xylitol production from plant biomass by Aspergillus niger through metabolic engineering.

Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass.

Clinical profile of fatal familial insomnia: phenotypic variation in 129 polymorphisms and geographical regions.

Objective: Elucidate the core clinical and genetic characteristics and identify the phenotypic variation between different regions and genotypes of fatal familial insomnia (FFI).

Methods: A worldwide large sample of FFI patients from our case series and literature review diagnosed by genetic testing were collected. The prevalence of clinical symptoms and genetic profile were obtained, and then the phenotypic comparison between Asians versus non-Asians and 129Met/Met versus 129Met/Val were conducted.

Elevated Serum Levels of Carbohydrate Antigen 72-4 in Diabetic Kidney Disease.

The aim of this study was to determine whether carbohydrate antigen 72-4 (CA72-4) is elevated in diabetic kidney disease (DKD), and examine the association between urinary albumin-to-creatinine ratio (UACR) and CA72-4 in patients with type 2 diabetes mellitus (T2DM). Non-dialysis patients with T2DM (n=296) and 90 healthy controls were recruited in this study. CA72-4 level was measured by electrochemiluminescence immunoassay.

Molecular engineering to improve lignocellulosic biomass based applications using filamentous fungi.

Lignocellulosic biomass is an abundant and renewable resource, and its utilization has become the focus of research and biotechnology applications as a very promising raw material for the production of value-added compounds. Filamentous fungi play an important role in the production of various lignocellulolytic enzymes, while some of them have also been used for the production of important metabolites. However, wild type strains have limited efficiency in enzyme production or metabolic conversion, and therefore many efforts have been made to engineer improved strains.