Seeds are a major source of contamination by foodborne pathogens such as , , and , significantly increasing the risk of foodborne diseases associated with fresh produce like sprouts. In this study, we described novel endolysins and the engineered variants that exhibited potent bactericidal activity against these pathogens. These endolysins demonstrated strong bactericidal effects independently of outer membrane permeabilizers, effectively killing , , and to undetectable levels (>4-log kill) at concentrations as low as 12.
View Article and Find Full Text PDFObjective: This study aimed to investigate the effects of cellulase, protease, and starch on the fermentation quality, digestibility, and microbial community of mulberry leaf silage after 30d of ensiling.
Methods: Mulberry leaves (376 g/kg dry matter (DM)) were ensiled with four experimental treatments: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]); iii) CPR, added cellulase (120 U/g FM) and protease (50 U/g FM); and iv) CPS, added cellulase (120 U/g FM), protease (50 U/g FM), and starch (2% FM).
Results: All treatments with additives improved fermentation quality, showing higher DM (353 ~ 378 vs.
Ethnopharmacological Relevance: Qingjie Fuzheng Granules (QFG), a herbal formula, has been employed as an adjuvant therapy for colitis-associated colorectal cancer (CAC), yet the underlying mechanisms by which QFG operates remain unclear.
Aims Of The Study: The aim of this study is to investigate whether the potential mechanism of QFG against CAC is associated with macrophage polarization.
Materials And Methods: Non-targeted metabolomics and molecular docking assessed potential compounds of QFG to interact with targets associated with macrophage polarization.
Background And Aims: Visceral adipose tissue has been indicated closely connected with the severity of acute pancreatitis (AP). Visceral adiposity index (VAI) is a mathematical model that consists of waist circumference, body mass index, triglyceride and high-density lipoprotein cholesterol, which has been demonstrated to be a better indicator of visceral fat than other traditional indices. This study aimed to evaluate the relationship between VAI and the severity of AP.
View Article and Find Full Text PDFRBOH gene may regulate the resistance of Isatis indigotica Fort. to salt stress by mediating the production of HO. RBOH gene plays an important role in plant growth and development, abiotic and biotic stress response, and hormone signalling.
View Article and Find Full Text PDFInfection is among the most common causes of death in patients with acute myeloid leukemia (AML) after chemotherapy. The anti-tumor effect of the intestinal microbiota in patients with AML is increasingly being recognized. Tigecycline, a broad-spectrum antibiotics, plays a vital role in the anti-infection treatment of AML patients with neutropenia and accompanying infections.
View Article and Find Full Text PDFPhage-derived peptidoglycan hydrolases (i.e. lysins) are considered promising alternatives to conventional antibiotics due to their direct peptidoglycan degradation activity and low risk of resistance development.
View Article and Find Full Text PDFA study of 355 nm laser with high pulse energy across various types of atherosclerotic lesion models is presented. The 355 nm laser pulses (10 ns) are delivered via a single fiber (600 μm diameter), and the ablation of calcified tissue, lipid tissue, and thrombus-like tissue are studied under varied laser fluence (40-70 mJ/mm) and repetition rate (5-30 Hz). The contact and noncontact ablation processes of chicken tibia samples (calcified tissue) are compared at 60 mJ/mm and 30 Hz, and the size of ablation particles is in the range of 0.
View Article and Find Full Text PDFTo reveal the effect of sucrose concentration on the production of secondary metabolites, a metabolome and transcriptome joint analysis was carried out using callus induced from grape variety Mio Red cambial meristematic cells. We identified 559 metabolites-mainly flavonoids, phenolic acids, and stilbenoids-as differential content metabolites (fold change ≥2 or ≤0.5) in at least one pairwise comparison of treatments with 7.
View Article and Find Full Text PDFObjective: The purpose of this experiment was to explore the effect of adding cellulase and laccase on fermentation quality and microbial community in mixed silage of corn stover and wet brewer's grains. Try to a new approach for the proper preservation and utilization of the agro-industrial by-products (corn stover and wet brewer's grains).
Methods: The experiment was divided into four groups: CK (control), C (cellulase, 120 U/g fresh matter [FM]), L (laccase, 50 U/g FM), CL (cellulase 120 U/g FW and laccase 50 U/g FM), and the chemical composition, fermentation quality, microbial population and microbial community in mixed silage of corn stover and wet brewer's grains after 30 day's fermentation were determined.
At present, researches on brain fatigue recognition are still in the stage of single task and simple brain region network features, while researches on high-order brain functional network features and brain region state mechanisms during fatigue in multi-task scenarios are still insufficient, making it difficult to meet the needs of fatigue recognition under complex conditions. Therefore, this study utilized functional near-infrared spectroscopy (fNIRS) technology to explore the correlation and differences in the low-order and high-order brain functional network attributes of three task induced mental fatigue, and to explore the brain regions that have a major impact on mental fatigue. Self-training algorithms were used to identify the three levels of brain fatigue.
View Article and Find Full Text PDFDaily meteorological observation data of the early period (pre-1950) were critically important for investigating the long-term trends and multi-decadal scale variability of extreme climate events. The high-resolution surface air temperature (SAT) data for time period before 1950 are lacking in China. We extended the SAT observations of China back to 1840 through developing a pre-1950 daily SAT dataset.
View Article and Find Full Text PDFWe demonstrate (1) detectable halogen bonding is not critical for enabling light-driven radical generation from diaryliodonium salts and (2) radicals generated by this route can be captured by transition-metals for C-H arylation reactions. These results are the first step toward developing new metal-catalyzed aryl radical couplings without exogenous photocatalysts.
View Article and Find Full Text PDFChiral 1,2,3-triazoles are highly attractive motifs in various fields. However, achieving catalytic asymmetric click reactions of azides and alkynes for chiral triazole synthesis remains a significant challenge, mainly due to the limited catalytic systems and substrate scope. Herein, we report an enantioselective azidation/click cascade reaction of N-propargyl-β-ketoamides with a readily available and potent azido transfer reagent via copper catalysis, which affords a variety of chiral 1,2,3-triazoles with up to 99% yield and 95% ee under mild conditions.
View Article and Find Full Text PDFUnlabelled: The gene family known as the Lateral Organ Boundary Domain () is responsible for producing transcription factors unique to plants, which play a crucial role in controlling diverse biological activities, including their growth and development. This research focused on examining ' gene, owing to its significant ecological, economic, and nutritional benefits. Examining the gene family's member count, physicochemical characteristics, phylogenetic evolution, gene configuration, and motif revealed 41 gene family members spread across 8 chromosomes, with gene's full-length coding sequences (CDSs) ranging from 327 to 1737 base pairs, and the protein sequence's length spanning 109 ()-579 () amino acids.
View Article and Find Full Text PDFPassive radiative cooling (PRC) has been acknowledged to be an environmentally friendly cooling technique, and especially artificial photonic materials with manipulating light-matter interaction ability are more favorable for PRC. However, scalable production of radiative cooling materials with advanced biologically inspired structures, fascinating properties, and high throughput is still challenging. Herein, we reported a bioinspired design combining surface ordered pyramid arrays and internal three-dimensional hierarchical pores for highly efficient PRC based on mimicking natural photonic structures of the white beetle ' wings.
View Article and Find Full Text PDFACS Appl Mater Interfaces
April 2024
Radiative cooling fabrics have gained significant attention for their ability to enhance comfort without consuming extra energy. Nevertheless, sweat accumulation on the skin and diminishing cooling efficiency usually exist in the reported polymer cooling membranes. Herein, we report a universal method to obtain a calcium (Ca)-salt-enhanced fiber membrane with high infrared emission and hydrophilicity for efficient passive cooling and flame retardancy.
View Article and Find Full Text PDFMid-ultraviolet light (290-320 nm) can promote human vitamin D synthesis, which helps in the prevention and treatment of rickets and cartilage disease. For people who lack sufficient ultraviolet radiation all year round, it is significant to supplement the ultraviolet component in daily lighting sources. However, there are few luminous materials showing a combination of mid-ultraviolet light and white light emission on the market.
View Article and Find Full Text PDFis a widely used medicinal plant. The total length of the chloroplast genome was 151,390 bp, and it comprised a large single-copy (LSC, 83,432 bp) region, a small single-copy (SSC, 18,304 bp) region, and a pair of inverted repeats (IRs, 49,654 bp). A total of 130 coding genes were annotated, including 88 protein-coding genes, 8 rRNA genes, and 34 tRNA genes.
View Article and Find Full Text PDFRandom speckle patterns contain valuable information about the incident light. Researchers have successfully constructed spectrometers and wavemeters by utilizing the speckles generated by inter-mode interferences of a multimode fiber (MMF). However, cameras were often employed to record the speckle data in previous reports.
View Article and Find Full Text PDFBunge, a perennial herb belonging to the Ranunculaceae family, has been extensively used in traditional Chinese medicine. Documented in the Supplements to the Compendium of Materia Medica, its medicinal properties encompass a spectrum of applications, including heat clearance, detoxification, alleviation of oral/throat sores, earaches, eye pain, cold-induced fever, and vision improvement. Furthermore, is used in clinical settings to treat upper respiratory infections, pharyngitis, tonsillitis, esoenteritis, canker, bronchitis, etc.
View Article and Find Full Text PDFPurpose: This cross-sectional study aims to assess the level of academic burnout among Chinese medical students during the controlled COVID-19 pandemic and identify the potential demographic factors affecting academic burnout. It also explored the relationship between career calling, family functioning, resource support, and academic burnout, as well as investigated whether family functioning and resource support could moderate the relationship between career calling and academic burnout among Chinese medical students.
Methods: The study was conducted in five Chinese cities in 2021.