CTCNet: a fine-grained classification network for fluorescence images of circulating tumor cells.

The identification and categorization of circulating tumor cells (CTCs) in peripheral blood are imperative for advancing cancer diagnostics and prognostics. The intricacy of various CTCs subtypes, coupled with the difficulty in developing exhaustive datasets, has impeded progress in this specialized domain. To date, no methods have been dedicated exclusively to overcoming the classification challenges of CTCs.

Effect of Solution Treatment on the Microstructure and Elevated Temperature Tensile Properties of Forged Rene 41 Superalloy.

The effects of a solution treatment on the microstructure and elevated mechanical properties of the forged Rene 41 superalloy were investigated. The results indicate that the solution treatment temperature has a significant influence on the γ' structure and mechanical properties. The sub-solvus solution treatment resulted in the co-existence of residual primary coarse γ' precipitates and fine secondary γ' precipitates, while the super-solvus solution treatments led to the complete dissolution of the primary γ' precipitates and the precipitation of a nano-sized secondary spherical γ' precipitate.

Exploration and validation of a novel reactive oxygen species-related signature for predicting the prognosis and chemotherapy response of patients with bladder cancer.

Background: Reactive Oxygen Species (ROS), a hallmark of cancer, is related to prognosis, tumor progression, and treatment response. Nevertheless, the correlation of ROS-based molecular signature with clinical outcome and immune cell infiltration has not been thoroughly studied in bladder cancer (BLCA). Accordingly, we aimed to thoroughly examine the role and prognostic value of ROS-related genes in BLCA.

Potential involvement of cuproptosis induced by m6A-modified autophagy gene ATG10 in KICH : Cuproptosis induced by m6A-modified ATG10 in KICH.

Kidney Chromophobe (KICH) is the third most prevalent renal malignancy, with research challenges due to a dearth of cell lines and clinical samples. There is no specific treatment regimen tailored exclusively for KICH. This study employed gene expression analysis, immunohistochemistry (IHC), Spearman's correlation, immune cell infiltration assessment, and molecular network construction to investigate the autophagy gene ATG10 in KICH.

Long-term survival of a kidney transplant patient with advanced renal cancer after combination therapy: a case report and literature review.

renal transplant carcinoma, especially in the context of bilateral renal carcinoma, is rare and often presents as small, low-grade papillary renal cell carcinoma (RCC). There is currently no consensus or effective treatment for advanced metastatic RCC after kidney transplantation. A 40-year-old man developed renal transplant carcinoma with venous thrombus and lung metastases 13 years after transplantation.

Integrating scRNA-seq and Visium HD for the analysis of the tumor microenvironment in the progression of colorectal cancer.

Background: Colorectal cancer (CRC) development is a complex, multi-stage process, transitioning from normal to adenomatous tissue, and then to invasive carcinoma. Despite research, there's a knowledge gap on using high-resolution spatial omics to understand CRC's tumor microenvironment dynamics.

Methods: We used single-cell transcriptomics to study major biological changes and cell interactions in CRC progression.

A novel type III effector RipBU from Ralstonia solanacearum suppresses plant immunity and promotes peanut susceptibility.

A predicted peanut R. solanacearum T3E RS_T3E_Hyp6 was identified as a definite T3E and renamed as RipBU. It is relative conserved in 31 R.

Visible-light-programmed patterning in dynamically bonded cholesteric liquid crystal elastomer.

Optical properties of cholesteric liquid crystal elastomers (CLCEs) can be tuned by an external field, however, it will spontaneously restore to the original state after the field is removed. Here, we introduce diselenide dynamic covalent bonds (DCBs) into CLCEs, whose optical properties can be reversibly and precisely tuned under the combined action of force and light. The tuned optical properties will be written into and remembered by the CLCEs, thus a programming effect is achieved.

Integrated analysis of single-cell RNA sequencing and bulk transcriptome data identifies a pyroptosis-associated diagnostic model for Parkinson's disease.

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by an insidious onset. Despite the emphasis on motor symptom-based diagnosis, there remains an unmet clinical need for effective diagnostic approaches during the prodromal phase of PD. Recent advances in single-cell RNA sequencing (scRNA-seq) and bulk transcriptomic analyses of PD patients open avenues for identifying potential diagnostic biomarkers.

Metformin inhibits migration and epithelial-to-mesenchymal transition in non-small cell lung cancer cells through AMPK-mediated GDF15 induction.

The growth differentiation factor 15 (GDF15) may serve as a biomarker of metformin, which mediates the bodyweight lowering effect of metformin. However, whether GDF15 also serves as a molecular target of metformin to inhibit carcinogenesis remains largely unknown. This study examined the role and molecular mechanisms of GDF15 in the anticancer effects of metformin in non-small cell lung cancer (NSCLC) cells, which has never been reported before.

Concomitant gut dysbiosis and defective gut barrier serve as the bridges between hypercortisolism and chronic systemic inflammation in Cushing's disease.

Objective: The aim of this study was to investigate the gut microbial signatures and related pathophysiological implications in patients with Cushing's disease (CD).

Design And Methods: Twenty-seven patients with CD and 45 healthy controls were enrolled. Based on obtained metagenomics data, we performed correlation, network study, and genome interaction group (GIG) analysis.

Effects of clear aligners and traditional removable appliances on oral microbiome in mixed dentition: a comparative study.

Background: This prospective study aims to investigate the comparative effects of clear aligners (CA) and traditional removable appliances (RA) on the cariogenic risk of patients in mixed dentition, focusing on the oral microbiome.

Methods: 25 children were included and assigned into CA and RA groups. Supragingival plaque and saliva samples were collected, and clinical parameters including Decay-missing-filled teeth index (DMFT), Plaque Index (PI) and Gorelick Index (GI) were recorded before treatment (T) and after 6-month follow-up (T).

Development of a standardized and reproducible murine femoral distraction osteogenesis model.

Objective: Distraction osteogenesis (DO) has been widely used to treat bone defects as its effectiveness in bone regeneration. Currently, distraction devices for establishing DO models are mainly developed for rats or large animals. However, a mouse DO model is in great need for in-depth mechanistic investigations using various transgenic mice.

Plasma lipidome, circulating inflammatory proteins, and Parkinson's disease: a Mendelian randomization study.

Background: Observational studies have suggested that plasma lipidome play a pivotal role in the occurrence of Parkinson's disease (PD). However, it remains unknown which lipids among plasma lipidome affect PD and how they exert their influence. Clarity is lacking regarding the causal relationship between plasma lipidome and PD, as well as whether circulating inflammatory proteins serve as mediators.

Pyrrolic-Nitrogen Chemistry in 1-(2-hydroxyethyl)imidazole Electrolyte Additives toward a 50,000-Cycle-Life Aqueous Zinc-Iodine Battery.

Rechargeable aqueous zinc iodine (Zn-I) batteries offer benefits such as low cost and high safety. Nevertheless, their commercial application is hindered by hydrogen evolution reaction (HER) and polyiodide shuttle, which result in a short lifespan. In this study, 1-(2-hydroxyethyl)imidazole (HEI) organic molecules featuring pyrrole-N groups are introduced as dually-functional electrolyte additives to simultaneously stabilize Zn anode and confine polyiodide through ion-dipole interactions.

Degradation of a Bipolar Membrane in a Hybrid Acid/Alkali Electrolyzer Studied by X-ray Computed Tomography.

The use of a bipolar membrane (BPM) in a hybrid acid/alkali electrolyzer is widely considered as a promising energy technology for efficient hydrogen production. The stability of a BPM is often believed to be largely limited by the anion exchange layer (AEL) due to the hydrophilic attack of AEL polymers by hydroxide groups in alkaline. In this study, we employ X-ray computed tomography (CT) to investigate the degradation behaviors of BPM and found that the cation exchange layer (CEL) experiences more pronounced degradation compared with the AEL during water splitting operations.

Crystal structure and structure-guided tunnel engineering in a bacterial β-1,4-galactosyltransferase.

Lacto-N-neotetraose (LNnT), a representative oligosaccharide found in human milk, has been previously examined for its beneficial traits. However, the LNnT titer is limited by the efficient glycosyltransferase pathway, particularly with respect to the catalysis of rate-limiting steps. As data on the crystal structure of the key enzyme required for synthesizing LNnT are lacking, the synthesis of LNnT remains an uncertainty.

Deciphering the energy storage mechanism of CoS nanowire arrays for High-Energy aqueous copper-ion batteries.

Transition metal sulfide (TMs) offers ultra-high specific capacity through multi-electron transfer, showing promise for aqueous batteries. However, the poor cycling performance and the uncleared energy storage mechanism are restricted from further development. Herein, CoS nanowire arrays grown on carbon cloth (CoS/CC) are proposed as binder-free and self-supporting electrodes for aqueous copper-ion batteries.

LINC00461 promotes bladder cancer cells EMT through miR-518b/HNRNPUL1 axis.

Bladder cancer (BC) is a prevalent type of tumor in the urinary system, and it has been discovered that long non-coding RNA (lncRNA) plays a significant role in its occurrence and development. However, thus far, no reports have been published on the involvement of LINC00461 in BC. Here, we found that LINC00461 levels were upregulated in BC tissues and cell lines.

Risk of Parkinson's disease and depression severity in different populations: A two-sample Mendelian randomization analysis.

Background: Depression is widely recognized as a common non-motor symptom of Parkinson's disease (PD). Across different studies, the reported prevalence of depression in PD varies widely, ranging from 2.7% to 90%, but it is unclear whether this association is due to genetic or acquired factors.