Anti-breast cancer effect of Phellinus pini and its chemical composition characterization.

Phellinus pini (P. pini) has been widely utilized as a traditional medicinal macrofungus in China and various East Asian countries. Modern pharmacological studies have shown that P.

Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense.

Older individuals experience increased susceptibility and mortality to bacterial infections, but the underlying etiology remains unclear. Herein, it is shown that aging-associated reduction of commensal Parabacteroides goldsteinii (P. goldsteinii) in both aged mice and humans critically contributes to worse outcomes of bacterial infection.

Height development trends among 7-18-year-old school-age children in central plains of China between 2000 and 2019: A serial cross-sectional surveillance study in China.

Objective: The aim of this study was to analyze changes in height of 7-18-year-old school-age children in China during the year of 2000-2019.

Methods: We used the survey data from the Chinese National Survey on Students' Constitution and Health in Henan Province for the years 2000, 2005, 2010, 2014, and 2019. Data were categorized into subgroups based on geographic location, gender, and age; mean, standard deviation, and Pearson's correlation coefficient were used to analyze trends in height change among children and adolescents and the correlation between socioeconomic indicators and height change.

Secular Trends in the Median Age at Menarche and Spermarche Among Chinese Children From 2000 to 2019 and Analysis of Physical Examination Indicators Factor.

Objectives: This research aimed to understand the long-term trends in the age of menarche among girls and spermarche among boys, and to analyze the factors that may influence the onset of menarche and first spermarche.

Methods: The study was based on data from 49 566 students in Henan Province collected from the Chinese National Surveys on Students' Constitution and Health (CNSSCH) conducted between 2000 and 2019. Median age at menarche and spermarche was estimated by probit analysis.

Sucrose synthase: An enzyme with multiple roles in plant physiology.

Sucrose synthase (SuS) is a key enzyme in the regulation of sucrose metabolism in plants and participates in the reversible reaction of sucrose conversion to uridine diphosphate-glucose and fructose. It plays an important role in promoting taproot development, starch synthesis, cellulose synthesis, improving plant nitrogen fixation capacity, sugar metabolism, and fruit and seed development. Recent studies have shown that SuS responds to abiotic stresses such as drought stress, cold stress and waterlogging stress, especially in waterlogging stress.

Needs for discharge planning and facilitators-barriers to implementing discharge plans for patients with SLE: A qualitative study.

Objective: To investigate the needs of patients with SLE in terms of discharge planning, to outline the practical perspectives of clinical healthcare professionals regarding the enablers and impediments to SLE patients' discharge planning, and to establish a basis for the creation of SLE discharge plans for subsequently diagnosed patients.

Methods: Descriptive qualitative research methodology was used in this study, with the researcher herself as the research instrument. Healthcare professionals formally employed in the rheumatology and immunology department, as well as SLE patients admitted to a tertiary-level hospital in Anhui Province between August and December 2023, were chosen for the study using a purposeful sample technique.

In vitro silencing of RIP2 in naive CD4 T cells from lupus-prone mice promotes pathogenic Th17 cell differentiation.

Objective: This work aims to investigate whether RIP2 silencing in naive CD4 T cells from lupus-prone mice impacts Th17 cell activity or differentiation in vitro.

Methods: Naive CD4 T cells isolation from MRL/lpr mice's spleens. Three RNA interference target sequences of RIP2 were packaged with lentivirus and transfected into naive CD4 T cells.

[Effects of tree species assembly on bioavailable P components in rhizosphere soil of southern subtropical plantation].

Improving the availability of soil phosphorus (P) and promoting tree growth through tree species selection and assembly are the critical issue. We conducted an afforestation experiment following randomized block experimental design with 1, 2, 4, and 6 tree species richness in south subtropics, including , , , , , , , and . We measured the bioavailable P components (CaCl-P, citrate-P, enzyme-P and HCl-P) and examined the effects of different tree species assembly on bioavailable P components and tree growth.

Current investigation of the high prevalence of sexual dysfunction in female patients with systemic lupus erythematosus: a cross-sectional study.

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects women of childbearing age and has been reported to cause sexual dysfunction in women. Although there are articles on sexual function in women with SLE, the number of articles is small, and the factors affecting sexual function in women with SLE are controversial. Based on this, this study aimed to investigate the prevalence of sexual dysfunction in Chinese female SLE patients and to explore the factors that influence it.

Highly sensitive and specific detection of human papillomavirus type 16 using CRISPR/Cas12a assay coupled with an enhanced single nanoparticle dark-field microscopy imaging technique.

Human papillomavirus (HPV) is a prevalent sexually transmitted pathogen associated with cervical cancer. Detecting high-risk HPV (hr-HPV) infections is crucial for cervical cancer prevention, particularly in resource-limited settings. Here, we present a highly sensitive and specific sensor for HPV-16 detection based on CRISPR/Cas12a coupled with enhanced single nanoparticle dark-field microscopy (DFM) imaging techniques.

New flavonoid and their anti-A549 cell activity from the bi-directional solid fermentation products of Astragalus membranaceus and Cordyceps kyushuensis.

Astragalus membranaceus and Cordyceps kyushuensis were used to obtain Astragalus membranaceus-Cordyceps kyushuensis bi-directional solid fermentation products using the bi-directional solid fermentation technique. The fermentation products were isolated and purified to obtain 20 individual compounds, of which compound 1 was a novel isoflavane, and compounds 2, 3, and 4 were novel isoflavones, along with 16 known compounds. In vitro experiments demonstrated that compounds 4, 5, 8, 10, and 20 exhibited significant inhibitory activity against A549 lung cancer cells.

Wnt5 controls splenic myelopoiesis and neutrophil functional ambivalency during DSS-induced colitis.

Neutrophils are important innate immune cells with plasticity, heterogenicity, and functional ambivalency. While bone marrow is often regarded as the primary source of neutrophil production, the roles of extramedullary production in regulating neutrophil plasticity and heterogenicity in autoimmune diseases remain poorly understood. Here, we report that the lack of wingless-type MMTV integration site family member 5 (WNT5) unleashes anti-inflammatory protection against colitis in mice, accompanied by reduced colonic CD8 T cell activation and enhanced splenic extramedullary myelopoiesis.

Vitamin D Improves -Induced Severe Pneumonia in Rats by Regulating Intestinal Microbiota.

Background: In the context of progressively uncontrolled drug resistance of bacteria, the difficulty of treating (KP)-induced pneumonia increases. Searching for drugs other than antibiotics has become an urgent task. Vitamin D (VD), meanwhile, is shown to be capable of treating pneumonia.

A Compound Heterozygosis of Two Novel Mutations in vWF Exacerbates vWD in a Chinese Pedigree.

Background: von Willebrand disease (vWD), caused by mutations in the von Willebrand factor (vWF) coding gene, is a disease characterized by abnormal coagulation activity and a severe tendency for hemorrhage. Therefore, identifying mutations in vWF is important for diagnosing congenital vWD.

Methods: We studied a 23-year-old male vWD patient and his parents.

Benzosceptrin C induces lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting DHHC3.

Programmed cell death-1 (PD-1)/programmed cell death ligand-1 (PD-L1) blockade has become a mainstay of cancer immunotherapy. Targeting the PD-1/PD-L1 axis with small molecules is an attractive approach to enhance antitumor immunity. Here, we identified a natural marine product, benzosceptrin C (BC), that enhances the cytotoxicity of T cells to cancer cells by reducing the abundance of PD-L1.

Association between serum PCSK9 and coronary heart disease in patients with type 2 diabetes mellitus.

Background And Aims: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is considered a new biomarker for atherosclerosis, but its ability to predict cardiovascular outcomes has been controversial. This study aimed to address the lack of data on PCSK9, coronary heart disease (CHD) severity, and major cardiovascular events (MACEs) in patients with type 2 diabetes mellitus (T2DM).

Methods: A total of 2984 T2DM patients underwent selective coronary angiography, and their serum PCSK9 levels were measured using enzyme-linked immunosorbent assay.

Exploring the regulatory role of Linc00893 in asthenozoospermia: Insights into sperm motility and SSC viability.

The role of long intergenic noncoding RNA 00893 (Linc00893) in asthenozoospermia (AS) and its impact on sperm motility remains unclear The present study explored the effect of Linc00893 on AS, specifically its effect on sperm motility and its relationship with spermatogonial stem cell (SSC) vitality and myosin heavy chain 9 (MYH9) protein expression. Linc00893 expression was analyzed in semen samples using reverse transcription‑quantitative PCR, revealing a significant downregulation in samples from individuals with AS compared with those from healthy subjects. This downregulation was found to be negatively correlated with parameters of sperm motility.

Mechanical and Electromagnetic Wave Absorption Performance of Carbonyl Iron Powder-Modified Nonwoven Materials.

In order to develop carbonyl iron-enhanced electromagnetic wave-absorbing composites, this paper utilizes two different morphologies of carbonyl iron powder (CIP), spherical and flake-like, which are blended with aqueous polyurethane (PU) in three different ratios to prepare impregnating solutions. Polyester (PET) needle-punched nonwoven materials are impregnated with these solutions to produce electromagnetic wave-absorbing composites. First, electromagnetic parameters of the two CIP particle types, spherical carbonyl iron (SCIP) and flake-like carbonyl iron (FCIP), are tested with the coaxial method, followed by calculation of the results of their electromagnetic wave absorption performance.

De novo NAD synthesis contributes to CD8 T cell metabolic fitness and antitumor function.

The dysfunction and clonal constriction of tumor-infiltrating CD8 T cells are accompanied by alterations in cellular metabolism; however, how the cell-intrinsic metabolic pathway specifies intratumoral CD8 T cell features remains elusive. Here, we show that cell-autonomous generation of nicotinamide adenine dinucleotide (NAD) via the kynurenine pathway (KP) contributes to the maintenance of intratumoral CD8 T cell metabolic and functional fitness. De novo NAD synthesis is involved in CD8 T cell metabolism and antitumor function.

Intranasal dexmedetomidine improves postoperative sleep quality in older patients with chronic insomnia: a randomized double-blind controlled trial.

This study was determined to investigate the impact of intranasal dexmedetomidine (DEX) on postoperative sleep quality in older patients (age over 65) with chronic insomnia during their hospitalization after surgery. A randomized double-blind controlled trial was conducted to compare the effects of intranasal dexmedetomidine spray with a placebo group. The study was carried out at Xiangya Hospital, Central South University.