Nomogram predicting the cardiovascular disease mortality for older patients with colorectal cancer: A real-world population-based study.

Background: Cardio-oncology has received increasing attention especially among older patients with colorectal cancer (CRC). Cardiovascular disease (CVD)-specific mortality is the second-most frequent cause of death. The risk factors for CVD-specific mortality among older patients with CRC are still poorly understood.

Screening and verification of proteins that interact with HSPC238.

HSPC238 is a recently identified tumor suppressor and demonstrates ubiquitin ligase E3 enzyme activity. HSPC238 was found to be significantly downregulated in human hepatocellular carcinoma (HCC) in vivo and to inhibit the proliferation and invasion of hepatoma cells in vitro; however, the underlying molecular mechanism is largely unknown. In the present study, we screened for and identified proteins that physically interact with HSPC238.

PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells.

The gene for proteasome subunit alpha type-7 (PSMA7) is located in chromosomal 20q13.33, a region frequently amplified in tumor. In this study, we employed A549 human lung adenocarcinoma cells and showed that PSMA7 inhibits the proliferation, tumorigenicity and invasion of A549 cells in vitro.

Association of TGF-beta1, IL-4 and IL-13 gene polymerphisms with asthma in a Chinese population.

Background: Asthma is a common respiratory disease caused by genetic and environmental factors. It has been suggested that TGF-beta1, IL-4 and IL-13 play important roles in asthma.

Objectives: We attempted to confirm the roles of TGF-beta1, IL-4 and IL-13 polymorphisms in asthma in a Chinese population.

[Effect of UBE2C overexpression on the proliferation of 293T cell line].

Aim: To establish a 293T cell line with stable expression of UBE2C and to investigate the effect of UBE2C overexpression on the proliferation of 293T cells.

Methods: Recombinant plasmid pcDNA3.1(-)/UBE2C successfully constructed in previous time was transfected into 293T cells by lipofectamine 2000.

[Construction of recombinant eukaryotic expression vector pDsRed1-C3/XAPC7 and cellular localization of XAPC7 protein in different cell lines].

Aim: To construct the recombinant eukaryotic expression vector pDsRed1-C3/XAPC7 and to investigate the cellular localization of XAPC7 protein in 786-O and 293T and Chang liver cell lines.

Methods: The cDNA of XAPC7 was amplified from HBE135-E6E7 cell line by RT-PCR method. The aim gene fragment XAPC7 from pMD18-T/XAPC7 was subcloned into eukaryotic expression vector pDsRed1-C3.

[Association of the C3 gene polymorphisms with susceptibility to adult asthma].

Objective: To investigate the association between single nucleotide polymorphisms (SNPs) of the complement component 3 gene (C3) and adult asthma of Hans in southern China.

Methods: A case-control study was performed. Four hundred and eighty-four adult asthma patients diagnosed in Nanfang Hospital and Affiliated Hospital of Guangdong Medical College, and 553 healthy subjects were collected from 2006 to 2010 for the study.

[Association of single nucleotide polymorphisms of MD-1 gene with asthma in adults of Han Nationality in Southern China].

Objective: To investigate the correlation between MD-1 gene single nucleotide polymorphism (SNP) and bronchial asthma in adults of the Han nationality in Southern China as well as their lung functions.

Methods: From 2006 to 2010, 332 adult asthmatic patients of the Han nationality diagnosed in Nanfang Hospital, Southern Medical University, and 276 healthy volunteers were recruited for the study. The SNPs of MD-1 gene loci rs2233128 and rs7740529 were genotyped by using MassARRAY-IPLEX technology and matrix-assisted laser desorption ionization time of flight mass spectrometry platform (MALDI-TOF-MS).

[Construction of recombinant eukaryotic expression vector pDsRed1-C3/LOC51255 and subcellular localization of LOC51255 protein in HePG2 cell line].

Aim: To construct the recombinant eukaryotic expression vector pDsRed1-C3/LOC51255 and investigate the subcellular localization of LOC51255 protein in HePG2 cell line.

Methods: The cDNA of LOC51255 was amplified from the recombinant plasmid pET28b/LOC51255 by PCR. The aim gene fragment LOC51255 from pMD18-T/LOC51255 was subcloned into eukaryotic expression vector pDsRed1-C3.

[Molecular epidemiology of human caliciviruses diarrhea among infants and young children in Lanzhou from December 2001 to June 2004].

Objective: To investigate the characteristics of human caliciviruses (HuCV) diarrhea among infants and young children with acute diarrhea in Lanzhou, Gansu Province, China by using molecular epidemiologic techniques.

Methods: Stool specimens were collected from both outpatients and inpatients with acute diarrhea in Lanzhou. Enzyme-linked immunosorbant assay (ELISA) was used to detect rotavirus antigen (RVA).