[To explore the clinical significance of HBV large protein (HBV-LP) in diagnosing viral replication].

Objective: To explore the clinical significance of HBV large protein (HBV-LP) in diagnosing viral replication, we detected the HBV-LP, HBV DNA and the hepatitis B viral markers (HBV M) in the serum of the patients infected with HBV.

Methods: HBV-LP and HBV M were analyzed by using ELISA. HBV-DNA was quantitatively detected using real-time fluorescent polymerase chain reaction.

[Detection of anti-Sp17 antibodies in infertile patients' serum and its clinical significance].

Objective: To test anti-Sp17 antibodies in the serum of AsAb positive infertile patients, to investigate the proportion of anti-Spl7 antibodies in AsAb and their potential application to the serologic diagnosis of immune infertility and immunocontraception.

Methods: With human recombinant Sp17 as the antigen, the ELISA method was used to detect the positive rate, antibody titre and content of anti-Sp17 antibodies in the AsAb positive serum.

Results: The positive rate of anti-Sp17 antibodies in the AsAb positive serum was 56.

[Preparation and characterization of the monoclonal antibody against human sperm protein 17].

Aim: To prepare and characterize the monoclonal antibody (mAb) against sperm protein 17 (Sp17).

Methods: Human Sp17 cDNA was cloned and recombinant Sp17 protein was expressed as a fusion protein with an N-terminal 6-His tag. The purified recombinant Sp17 protein was used to immunize BALB/c mice for preparing mAb.