LncRNA NEAT1 promotes malignant phenotypes and TMZ resistance in glioblastoma stem cells by regulating let-7g-5p/MAP3K1 axis.

Glioblastoma multiforme (GBM) is one of the most malign brain tumors in adults. Temozolomide (TMZ) is an oral chemotherapy drug constituting the backbone of chemotherapy regimens utilized as first-line treatment of GBM. However, resistance to TMZ often leads to treatment failure.

[Clinical significance of BMP-2 protein and mRNA expression in human glioma].

Aim: To investigate the expression of BMP-2 protein and mRNA in glioma, and evaluate its clinical significance in clinicopathological status of patients with glioma.

Methods: BMP-2 protein and mRNA expression in 88 patients with glioma were examined by SABC immunohistochemistry and RT-PCR, respectively.

Results: The positive expression rate of BMP-2 protein in glioma tissues were 78.

[Electrocorticography monitoring in microsurgical treatment of solitary cavernous angiomas].

Objective: To investigate the curative effect of electrocorticography (ECoG) monitoring in the microsurgical treatment of cavernous angiomas.

Methods: Clinical data of 71 patients with epileptogenic cavernous angiomas,who had been performed ECoG monitoring during the operation,were analyzed retrospectively.

Results: The foci of cavernous angiomas and epilepsy of the 71 patients were resected during the operation.

[Thrombin preconditioning reduces brain injury caused by intracerebral infusion of high dose thrombin].

Objective: To explore the effect of the cerebral thrombin preconditioning on the thrombin-induced brain edema, to detect the expression of tumor necrosis factor-alpha (TNF-alpha), and to analyse the relationship between TNF-alpha and the thrombin-induced brain edema.

Methods: Forty SD rats were randomly divided into a ST group and a TT group. The rats received 50 L saline (ST group) or 1 U thrombin infusion (TT group), and received the second infusion (10 U thrombin) 24 h later.

[Chemoresistance of CD133(+) tumor stem cells from human brain glioma].

Objective: To explore the multidrug resistance (MDR) mechanism of ABC superfamily transporters in the tumor stem cells(TSC) from human brain glioma tissues.

Methods: Samples of glioma were obtained from 30 patients undergoing microsurgical tumor resection. The CD133(+) cells and CD133(-) cells from these tumor specimens were isolated by magnetic activated cell sorting(MACS).

[Isolation and identification of brain tumor stem cells from human brain neuroepithelial tumors].

Objective: To establish a simplified culture system for the isolation of brain tumor stem cells (BTSCs) from the tumors of human neuroepithelial tissue, to observe the growth and differentiation pattern of BTSCs, and to investigate their expression of the specific markers.

Methods: Twenty-six patients with brain neuroepithelial tumors underwent tumor resection. Two pieces of tumor tissues were taken from each tumor to be dissociated, triturated into single cells in sterile DMEM-F12 medium, and then filtered.

[Mononostril-septum-transsphenoidal approach for pituitary adenoma].

Objective: To summarize the mononostril-septum-transsphenoidal approach for pituitary adenoma.

Methods: The clinical features, operative techniques, and outcome of 36 patients with pituitary adenoma were analyzed retrospectively.

Results: Tumors were totally removed in 28 cases, and subtotally resected in 8 patients.

[Correlation research between cancer stem cells and the pathological grades of neuroepithelial tumors].

Objective: To explore the methods of isolation, culture and identification of brain tumor stem cells (BTSCs) in neuroepithelial tumor tissues in vitro, and to study the correlation between BTSCs and the patholorical grades of neuroepithelial tumors.

Methods: Tumor cells from patients undergoing neuroepithelial tumors excision were acutely dissociated, triturated into single cells, and then seeded into serum-free medium. After the primary brain tumor spheres (BTSs) were generated, they were triturated again and passaged in fresh medium.

[Isolation and characterization of brain tumor stem cells in human medulloblastoma].

Background & Objective: Tumor stem cells have been isolated from several kinds of solid tumors, including primary brain tumors such as glioma and medulloblastoma. This investigation was to establish a simplified culture system to isolate and passage brain tumor stem cells (BTSCs) from human medulloblastoma, observe their proliferation and differentiation, and determine the expression of normal neural stem cell antigens, CD133 and Nestin, in BTSCs.

Methods: Eleven specimens of medulloblastoma were acutely dissociated and triturated into single cells without trypsin digestion.

[Microsurgical treatment for craniopharyngioma combined transorbital-subfrontal and temporal craniotomy].

Objective: To summarize the experience in microsurgical removal of craniopharyngioma using combined transorbital-subfrontal and temporal craniotomy.

Methods: Eighteen patients with craniopharyngioma varied from 3.1 cm to 6.

[Keyhole microsurgery for acoustic neurinomas through suboccipital retrosigmoid sinus approach].

Background And Objective: Acoustic neurinomas is one of the most common benign tumor in central nervous system, the main treatment is surgical resection. This study is to explore keyhole surgery with suboccipital retrosigmoid sinus approach for acoustic tumor resection and discuss how to improve the surgical skill and effect.

Methods: Thirteen cases of acoustic neurinomas from Sept.