Plasmid-mediated azithromycin resistance in non-typhoidal Salmonella recovered from human infections.

Objectives: Mechanisms of non-typhoidal Salmonella (NTS) resistance to azithromycin have rarely been reported. Here we investigate the epidemiology and genetic features of 10 azithromycin-resistant NTS isolates.

Methods: A total of 457 NTS isolates were collected from a tertiary hospital in Guangzhou.

Cross talk between Cu excess and Fe deficiency in the roots of rice.

Copper (Cu) and iron (Fe) share similar characteristics and participate as coenzymes in several physiological processes. Both Cu excess and Fe deficiency result in chlorosis, however, the crosstalk between the two is not clear in rice. In this study, we performed transcriptome analysis for Cu excess and Fe deficiency in rice.

Transcripts related with ammonium use and effects of gibberellin on expressions of the transcripts responding to ammonium in two invasive and native species.

Soil nitrogen forms are important for exotic plant invasions. However, little effort has been made to study the molecular mechanisms underlying the utilization of different N forms in co-occurring invasive and native plants. The invasive plant prefers nitrate relative to ammonium, and mainly invades nitrate-dominated environments, while it co-occurring native congener prefers ammonium.

Fabrication of cellulose derivative coated spherical covalent organic frameworks as chiral stationary phases for high-performance liquid chromatographic enantioseparation.

Porous spherical silica-based chiral stationary phases (CSPs) have been commercially used in the field of chiral separation, however, the scope of their application is, to some extent, limited by the instability of silica towards mobile phase containing strong base or acid. As such, developing new matrix-based CSPs is one of the effective strategies to overcome this bottleneck in studies of chiral separation materials. In this work, we have demonstrated that stable spherical covalent organic frameworks (SCOFs) can be utilized as matrixes for the fabrication of new CSPs for the first time.

Joint effect of pre-operative anemia and perioperative blood transfusion on outcomes of colon-cancer patients undergoing colectomy.

Background: Both pre-operative anemia and perioperative (intra- and/or post-operative) blood transfusion have been reported to increase post-operative complications in patients with colon cancer undergoing colectomy. However, their joint effect has not been investigated. The purpose of this study was to evaluate the joint effect of pre-operative anemia and perioperative blood transfusion on the post-operative outcome of colon-cancer patients after colectomy.

[Molluscicidal effect and mechanism of action of niclosamidate against ].

Objective: To evaluate the molluscicidal effect of niclosamidate against in laboratory and explore its mechanism by determining the enzyme activities of six important enzymes in snail soft tissues.

Methods: snails were treated with niclosamidate at the concentration of 1.25 mg/L for 24 h and the snail soft tissues were separated and prepared for analysis.

[Epidemiological investigation on fascioliasis and its risk factors in population of Binchuan County, Yunnan Province].

Objective: To understand the infection status of Fasciola and related risk factors in population of Binchuan County, Yunnan Province.

Methods: In February and March, 2014, 6 natural villages from 2 administrative villages in Zhoucheng Town were selected as investigated sites, and the permanent residents aged ≥ 5 years were examined by ELISA for the serum antibody detection, and then the positive ones were examined by Kato-Katz technique and the hatching method for Fasciola egg detection. Meanwhile, the fascioliasis cases that checked out by the above methods were further surveyed, and the residents who with the same gender, similar age and negative results in the blood examination were selected as the controls, then the risk factors were explored through a case control study.

[Research of freely grazing and wild feces behaviors in schistosomiasis endemic areas of plateau mountain area in Yunnan Province].

Objective: To understand the situation of the freely grazing and wild feces behaviors of residents in plateau mountain area of schistosomiasis endemic areas in Yunnan Province.

Methods: Two villages of Xidian and Moguang in Heqing County, Yunnan Province were selected as the study area and the questionnaire surveys were performed to the randomly selected villagers aged 6 to 65 years with the sampling ratio of 30%. Then the respondents were tested for the infection of schistosomiasis by indirect hemagglutination assay (IHA).

[Research of water contact and labor practice of residents in plateau mountain schistosomiasis endemic areas in Yunnan Province].

Objective: To understand the status of water contact and labor practice of residents in plateau mountain schisto- somiasis endemic areas in Yunnan Province, so as to provide the evidence for formulating the strategy of prevention and control of schistosomiasis in the next step of work.

Methods: A total of 412 residents aged 6-65 years old in 2 villages were randomly sampled and investigated with questionnaires for their water contacts and labor practices in plateau valley schistosomiasis endem- ic areas. Then the schistosome infection status of the residents was surveyed with the indirect hemagglutination assay (IHA).

[Cloning, Expression and Immunodiagnostic Evaluation of the Fasciola gigantica Thioredoxin Peroxidase].

Objective: To immunoscreen the gene encoding thioredoxin peroxidase (TPx) from a cDNA library made from adult Fasciola gigantica worms, clone and express the gene, and evaluate the immunodiagnostic value of TPx recombinant protein.

Methods: The A ZAP cDNA library was immunoscreened with pooled serum of fascioliasis gigantica patients. The obtained positive clones were sequenced and analyzed by multiple sequence alignment.

[Effect of comprehensive schistosomiasis control measures based on infection source control in mountainous areas of Yunnan Province].

Objective: To evaluate the effect of comprehensive schistosomiasis control measures based on infection source control in plateau mountain areas of Yunnan Province.

Methods: From 2006 to 2004, four administrative villages were selected as test areas from plateau canyon and plateau basin endemic areas in Jindun Town, Heqing County, two villages each type, and the comprehensive control measures were implemented, including the examination and treatment of schistosomiasis, Oncomelania hupensis snail survey and control, health education, improving drinking water and lavatories, banning grazing, constructing sanitary pen of livestock, replacing cattle with machine, etc. The schistosome infection state and snail status in 2006 were treated as the baseline information, and the effect of the comprehensive measures were evaluated.

[Epidemiological investigation of fascioliasis and analysis of a chronic human fascioliasis case in Binchuan County, Yunnan Province].

From February to March 2014, six natural villages in Zhoucheng Town, Binchuan County of Yunan Province, were randomly selected by cluster sampling. Serum anti-fascioliasis IgG was detected by ELISA. The sero-positive individuals were further tested for Fasciola infection using sediment detection with nylon bag (260 meshes) and Kato-Katz method.

An Outbreak of Human Fascioliasis gigantica in Southwest China.

Fascioliasis is a common parasitic disease in livestock in China. However, human fascioliasis is rarely reported in the country. Here we describe an outbreak of human fascioliasis in Yunnan province.

[Effect of comprehensive control and management of schistosomiasis at plateaus regions in Yunnan Province].

Objective: To evaluate the effect of comprehensive control and management of schistosomiasis at plateaus regions in Yunnan Province.

Methods: Xiaolian and Kangfu villages at plateaus regions where schistosomiasis was endemic were selected as study areas from 2006 to 2011, the schistosomiasis comprehensive control measures were conducted, and these measures included the health education, chemotherapy, stool and water management, grazing forbidden, Oncomelania snail control, etc. combined with the infrastructure of farmland water conservancy, agricultural comprehensive development, adjustment of industry structure, returning farmland to forest, ditch hardening, and aquaculture.

[Investigation on outbreak of angiostrongyliasis cantonensis due to consumption of snail food in Dali City].

Objective: To investigate the potential cause of an outbreak of eosinophilic meningitis in Dali City in March, 2011.

Methods: A retrospective investigation was performed among 18 consumers who had meal together. The clinical documents of inpatients were analyzed and the diagnoses were checked.