Analysis of 602 Chinese cases of nevus of Ota and the treatment results treated by Q-switched alexandrite laser.

Background: Nevus of Ota is a congenital or acquired pigmentary disorder of the skin and mucous membranes, which are areas innervated by the first and second branches of the trigeminal nerve. Nevus of Ota is very common in Asia. Nevus of Ota was effectively treated with Q-switched alexandrite (755 nm) laser, but no detailed report existed on many Chinese cases treated with Q-switched alexandrite laser.

Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.

Objective: To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).

Methods: HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).

[Effectiveness and safety of long-pulsed Alexandrite laser for hair removal in 1702 patients].

Objective: To evaluate the effectiveness and safety of long-pulsed Alexandrite laser for hair removal.

Methods: Hair removal was performed in 1702 hirsute patients with long-pulsed Alexandrite laser. Among them 1603 patients received two or more operations.

[Flashlamp-pumped pulsed dye laser (585 nm) in the treatment of port-wine stains--a retrospective study of 2317 Chinese patients].

Objective: To evaluate the efficacy and safety of 585 nm flashlamp-pumped pulsed dye lasers (PDL) in the treatment of port-wine stains (PWS).

Methods: A retrospective review was performed in 2 317 patients with PWS who visited the Dermatology Laser Centre of PUMC Hospital and accepted treatment with 585 nm PDL. The correlation between the treatment efficacy and the treatment sessions, lesion types, and usage of other therapies were analyzed.

[Clinical efficacy of Q-switched Alexandrite laser for pigmentary skin diseases in 4656 patients].

Objective: To evaluate the clinical efficacy and safety of Q-switched Alexandrite laser in the treatment of pigmentary skin, diseases ( PSDs).

Methods: Totally 4 656 patients with PSDs were treated with Q-switched Alexandrite laser. These PDSs included nevus of Ota, seborrheic keratosis, tattoo, naevus fusco-caeruleus zygomaticus, cafe-au-lait-spots, lentigo, naevus of Ito, and spilus naevus.

Correlation between deoxyribonucleic acid loads of human papillomavirus and recurrence of condylomata acuminata.

To determine the correlation between deoxyribonucleic acid (DNA) loads of human papillomavirus (HPV) and recurrence of condylomata acuminata (CA), 31 cases of primary CA and 32 cases of recurrent CA were assayed for the HPV6/11 and HPV16/18 DNA loads by real-time fluorogenic quantitative PCR. The results showed 62 of the 63 cases were HPV6/11 DNA positive (98.4%).

[Correlation between DNA load of human papillomavirus and recurrence of condyloma acuminata].

Objective: To determine the correlation between DNA load of human papillomavirus (HPV) and recurrence of condyloma acuminata (CA).

Methods: The HPV6/11 and HPV16/18 DNA load of 31 cases of primary CA and 32 cases of recurrent CA were detected by real-time fluorogenic quantitative PCR.

Results: Among the 63 CA patients, 62 cases were HPV6/11 DNA positive.

[Immunogenicity of mutant and wild HPV16 DNA vaccines].

Objective: To evaluate the efficacy of mutation of human papillomavirus 16 (HPV16) E7 in two zinc-binding motifs on HPV16 E7 C terminus on antigen-specific immunity.

Methods: pcDNA3.1/16E7 and pcDNA3.

Construction and immunogenicity of human papillomavirus type 6b L1 recombinant plasmid.

Objective: To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immunogenicity in mice.

Methods: The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic expression plasmid (pcDNA3.1).

[Detecting HPV DNA in tissues of external genital squamous cell carcinoma in situ by PCR-RFLP technique].

Objective: To study the relationship between the infection of human papillomavirus (HPV) and external genital squamous cell carcinoma in situ.

Methods: HPV DNA was detected with the consensus primer by polymerase chain reaction (PCR) and its type was determined by restriction fragment length polymorphism (RFLP).

Results: HPV DNA was detected and HPV16 was the most frequently identified type in 29 (56.

[Analysis of 602 cases of nevus of Ota and study of ultrastructures on the melanocytes].

Objective: To analyze clinical information and efficacy of 602 cases of nevus of Ota, and investigate the histopathology and ultrastructure on the melanocytes before and after Q-switched Alexandrite laser irradiation.

Methods: Clinical information of 602 cases of nevus of Ota were collected by applying clinical records, checking photos, and inquiry to patients by letters and telephones. Ten cases of biopsies were observed by light microscopy and 6 cases by electron microscopy before and after laser irradiation.

[Identification and assessment of multiple human papillomavirus types in condyloma acuminata lesions from patients with genital warts in Beijing area].

Objective: To identify and assess multiple human papillomavirus types in condyloma acuminatum lesions from patients with genital warts in Beijing area, and compare different features between otherwise healthy and immunosuppressed patients.

Methods: PCR, RFLP and nucleotide sequencing analysis were used to determine HPV types from individual lesions.

Results: The predominant type from other healthy patients was HPV6, secondly HPV11.