Rapid discrimination and quantification of chemotypes in Perillae folium using FT-NIR spectroscopy and GC-MS combined with chemometrics.

Perillae Folium (PF) is a well-known food and herb containing different chemotypes, which affect its quality. Herein, a method was proposed to classify and quantify PF chemotypes using gas chromatography-mass spectrometry (GC-MS) and Fourier transform-near infrared spectroscopy (FT-NIR). GC-MS results revealed that PF contains several chemotypes, including perilla ketone (PK) type, -asarone (PP-as) type, and dillapiole (PP-dm) type, with the PK type being the predominant chemotype.

MRGPRX2 is critical for clozapine induced pseudo-allergic reactions.

Background: Clozapine is one of the most widely used second-generation antipsychotics in clinic. However, allergy-like symptoms such as rash and angioedema have been reported frequently, and the mechanism is still not clear. Mas-related G protein-coupled receptor X2 (MRGPRX2) expressed on mast cells is a crucial receptor for drug induced pseudo-allergic reactions.

[Effects of interplanting on soil aggregate distribution and stability in economic orchard at hilly slope red soil of southern China].

We investigated the effects of interplanting on soil aggregate distribution and stability of red soil in economic orchard in a new-constructed slope land, based on 12 kiwifruit planting experi-mental plots with a slope of about 12°, a length of 18 m, and a width of 1.5 m. Three types of interplanting patterns were implemented by interplanting purple sweet potato (PSP), hairy vetch (HV), and weeds (W) for three years, respectively, taking the bare land with no vegetation as control (CK) to determine the aggregate indicators at 0-15 cm soil layer.

Induction of Osteogenic Differentiation of Human Adipose-Derived Stem Cells by a Novel Self-Supporting Graphene Hydrogel Film and the Possible Underlying Mechanism.

Graphene and its derivatives have received increasing attention from scientists in the field of biomedical sciences because of their unique physical properties, which are responsible for their interesting biological functions. With a range of extraordinary properties such as high surface area, high mechanical strength, and ease of functionalization, graphene is considered highly promising for application in bone tissue engineering. Here, we examined the effect of using a self-supporting graphene hydrogel (SGH) film to induce the osteogenic differentiation of human adipose-derived stem cells (hADSCs).

Maxillary sinus floor elevation with a tissue-engineered bone composite of deciduous tooth stem cells and calcium phosphate cement in goats.

The study aimed to assess the effect of maxillary sinus floor elevation with tissue-engineered bone constructed from deciduous tooth stem cells (DTSCs) and calcium phosphate cement (CPC). The stem cells from goat deciduous teeth (SGDs) were isolated and transfected by means of the adenovirus with an enhanced green fluorescent protein gene (AdEGFP). As many as 18 bilateral maxillary sinuses of nine goats were randomly allocated into three groups (n = 6/group): group A (SGDs-CPC compound), group B (CPC alone) and group C (autogenous bone obtained from an iliac crest).

[Preliminary study of akermanite's effect on stem cells from goat deciduous teeth in proliferation and osteogenic differentiation].

Purpose: To explore the characters of attachment, proliferation and osteogenic differentiation of stem cells from goat deciduous teeth (SGDs) cultured on akermanite ceramic.

Methods: SGDs were collected with enzyme digestion, and cultured. Under culture condition in growth medium, actin filament labeling and CCK-8 analysis were used to reveal attachment and proliferation.

[Ectopic osteogenesis of SGDs in goat's muscle pouch].

Purpose: To investigate ectopic osteogenesis of composites SGDs with porous calcium phosphate cement(pCPC) in goat's muscle pouch.

Methods: SGDs were cultivated with modified tissue culture techniques, then were induced into osteoblasts in the third passage, the osteogenic-induced SGDs were combined with pCPC and transplanted into the goat left dorsal muscle pouch, the pCPC without cells was transplanted into the right dorsal muscle pouch as negative controls. The transplants were harvested at 2-,4-,6-,8- week and prepared for histological examination.

[Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis].

Purpose: To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth (SGDs) with porous calcium phosphate cement (pCPC).

Methods: The expression of STRO-1 on SGDs was measured with flow cytometry (FCM); the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days. Combined with pCPC, the adhesion and growth of the compounds were observed with scanning electron microscopy (SEM); the ectopic bone-like tissue formation was observed 8 weeks after the compounds implanted subcutaneously into the nude mice.

[Isolation, cultivation and induced-mineralization of dental pulp cells from goat deciduous teeth in vitro].

Purpose: To isolate and cultivate dental cells from goat deciduous teeth ,and explore changes of its biological characters before and after induced-mineralization.

Methods: Pulp cells were cultivated with modified tissue block enzymolytic method, cell lineage in the second passage with SAB methods was checked out. Induced-mineralized cultivation was adopted in the fourth passage, some examinations were used to compare with normal cultivated cells: cell proliferative capality, mineralized ability test, cell morphology change, protein(OCN) expression level, related osteogenic genes(ALP,COL-I,OCN,OPN) expression.

[Culture and green fluorescent protein gene transfection of pulp cells from goat deciduous teeth in vitro].

Purpose: To isolate and culture pulp cells from goat deciduous teeth, and transfect green fluorescent protein gene.

Methods: Pulp cells from goat deciduous teeth were obtained by tissue culture. Cell growth curve was measured by counting the number of cells, HE and alkaline phophatase(AKP) stain, as well as immunhistochemical stain of vimentin and keratin were performed.

[A study of osteogenic induction of dental pulp stem cells from deciduous teeth in vitro].

Unlabelled: URPOSE: To isolate and culture dental pulp stem cells from human deciduous teeth, and induce them to osteoblasts.

Methods: Dental pulp stem cells were separated from deciduous teeth with enzyme digestionìthen isolated and purified by limited dilution. The cells clone form rate was carried out, and cell growth curve was measured by counting the number of cells.