The plastomes of Cypripedium (Orchidaceae: Cypripedioideae) exhibit atypical GC content and genome size based on different sequencing strategies.

Most of the sequenced plastomes of photosynthetic angiosperms exhibit conservation in size, gene content, gene order, and GC content. In contrast, the sequenced plastomes of Cypripedium are distinguished by genome size expansion, AT-biased base composition, structural variation, and a low substitution rate. Additionally, the impact of sequencing methods is seldom addressed in prior studies, and the species represented in these studies are underrepresented.

Endoscopic "calabash" ligation and resection for small gastric mesenchymal tumors.

Background: Gastric mesenchymal tumors (GMT) are identified as soft tissue neoplasms that arise from mesenchymal stem cells within the gastrointestinal tract. GMT primarily encompass gastric stromal tumors (GST), gastric leiomyomas, and gastric schwannomas. Although most GMT are benign, there are still potential malignant changes, especially GST.

Multichromosomal Mitochondrial Genome of : Compact and Fragmented Genome, and Rampant Intracellular Gene Transfer.

Orchidaceae is one of the largest families of angiosperms. Considering the large number of species in this family and its symbiotic relationship with fungi, Orchidaceae provide an ideal model to study the evolution of plant mitogenomes. However, to date, there is only one draft mitochondrial genome of this family available.

B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma.

Background: Beta-1,3-galactosyltransferase-4 (B3GALT4) plays a critical regulatory role in tumor biology. However, the role of B3GALT4 in modulating the tumor microenvironment (TME) of neuroblastoma (NB) remains unknown.

Methods: Public datasets and clinical NB samples were collected to evaluate the expression and clinical significance of GD2 and B3GALT4 in NB patients.

The chloroplast genome evolution of Venus slipper (Paphiopedilum): IR expansion, SSC contraction, and highly rearranged SSC regions.

Background: Paphiopedilum is the largest genus of slipper orchids. Previous studies showed that the phylogenetic relationships of this genus are not well resolved, and sparse taxon sampling documented inverted repeat (IR) expansion and small single copy (SSC) contraction of the chloroplast genomes of Paphiopedilum.

Results: Here, we sequenced, assembled, and annotated 77 plastomes of Paphiopedilum species (size range of 152,130 - 164,092 bp).

Chloroplast Genomes of Two Species of : Expanded Genome Size and Proliferation of AT-Biased Repeat Sequences.

The size of the chloroplast genome (plastome) of autotrophic angiosperms is generally conserved. However, the chloroplast genomes of some lineages are greatly expanded, which may render assembling these genomes from short read sequencing data more challenging. Here, we present the sequencing, assembly, and annotation of the chloroplast genomes of and .

Efficient Editing of an Adenoviral Vector Genome with CRISPR/Cas9.

Immunotherapy based on genetic modification of T cells has played an important role in the treatment of tumors and viral infections. Moreover, adenoviral vectors engineered with improved safety due to their inability to integrate into the host genome have been key in the clinical application of T cell therapy. However, the commonly used adenoviral vector Ad5 exhibits low efficiency of infection of human T cells and the details of the intracellular trafficking pathway of adenoviral vectors in human primary T cells remains unclear.

Increased plasma lipoprotein-associated phospholipase A2 levels are associated with coronary slow flow.

Objective: Coronary slow flow (CSF) is characterized by delayed opacification of distal epicardial coronary arteries without significant coronary stenosis. In addition, The changes of lipoprotein-associated phospholipase A2 (Lp-PLA) as a significant predictive factor for CSF remain controversial. The study aims to investigate the association between plasma Lp-PLA and CSF.