Publications by authors named "Jia-Kun Zheng"
Objective: To investigate the effect of electroacupuncture(EA) on miR-34a-5p, silent mating type information regulation 2 homolog-1 (SIRT1) and nuclear factor-κB subunit p65 (NF-κB p65) in the trigeminal ganglion of rats with migraine, so as to explore the mechanisms of EA underlying prevention of migraine.
Methods: Male SD rats were randomly divi-ded into normal, sham operation, model, EA, and EA plus EX527(a SIRT1 inhibitor) groups, with 10 rats in each group. The rat model of migraine was established by electrical stimulation of the trigeminal ganglion.
Article Synopsis
- A study was conducted in Wuxi, Jiangsu Province, China, where 10,297 healthy individuals underwent screening for hemoglobinopathies using various advanced genetic testing methods like Hb electrophoresis and PCR.
- Five individuals were identified as carriers of α-thalassemia, with specific deletions noted, along with several cases of β-thalassemia and other hemoglobin variants, leading to a documented low prevalence of these conditions in the population.
- Eight different genotypes of hemoglobin variants were discovered, including notable forms such as Hb E and Hb J-Bangkok, with a unique identification of a Sicilian δβ(0)-thal variant, marking its first report in Asia.
Background: Human papillomavirus (HPV) infection is the main cause of cervical cancer. Limited epidemiologic data of HPV prevalence are available for women attending hospitals in southern China. This study aimed to evaluate the profiles of HPV infection and cytology status in gynecological outpatients in Chaozhou City.
View Article and Find Full Text PDFArticle Synopsis
- HPV infection is the main cause of cervical cancer, with a study conducted on rural women in Chaozhou, China, revealing a 7.89% high-risk HPV prevalence among 48,559 cases.
- The study identified age (55-60 years) as a peak incidence group, and found that the most common HPV types were 52, 16, and 58, with a significant portion (48.23%) of infections involving types 52 and 58 combined.
- Results suggest a low prevalence of HPV vaccine types 16 and 18, indicating the potential benefit of developing second-generation HPV vaccines targeting types 52 and 58 for better protection in this rural population.
Gliomas are the most common tumors of the central nervous system (CNS) and a frequent cause of death. The treatment of malignant gliomas is often palliative due to their high recurrence rate. A growing body of evidence suggests that glioma may arise from cancer stem cells (CSC) correlated with neural stem cells (NSC), with the capacity for self-renewal and multipotency.
View Article and Find Full Text PDFThe cellular origin of soft tissue sarcomas (STSs) is not fully understood. The cancer stem cell hypothesis presumes that tumors originate from the malignant transformation of stem cells. As a type of multipotent stem cell, adipose tissue-derived stromal/stem cells (ADSCs), which possess an unexpected degree of plasticity and often reside in other tissues, may represent a potential source of soft tissue sarcoma.
View Article and Find Full Text PDFThis study investigated the inhibitory capacity of oxymatrine on in vitro hepatitis B virus (HBV) replication. HepG2.2.
View Article and Find Full Text PDF[Human amnion cells express the phenotypes of neural cells and adipocytes in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban
September 2006
Objective: To search for culture conditions in which the cells from human amnion could diferentiate into neural cells and hence to explore a new cell source for neural transplantaion.
Methods: Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
Results: Amnion cells migrated from explants and primary culture was established; they could multiply and expand steadily in a short time, and they could be passaged by trypsin digestion.
[Long-term culture and differentiation of human glioma stem cells ].
Sichuan Da Xue Xue Bao Yi Xue Ban
January 2006
Objective: To explore the culture and subculture conditions for glioma stem cells(GSCs) and to investigate the differentiation potential of GSCs.
Methods: The cells from human glioma were mechanically dissociated. Cells were cultured in N2 or B27 medium with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and they were identified by immunocytochemistry.
[Intraventricular transplantation of human neural stem cells into embryonic rats].
Sichuan Da Xue Xue Bao Yi Xue Ban
September 2005
Objective: To explore the migration and distribution of human neural stem cells (NSCs) after they were transplanted into lateral cerebroventricles of embryonic rats.
Methods: This study was conducted with the proxy consent and the permission from the Hospitals Ethical Review Committee. The cells from the embryonic human cortices were mechanically dissociated.
Objective: To investigate the differentiative capability of adult human bone marrow mesenchymal stem cells (BMSCs) into Schwann-like cells.
Methods: Bone marrows were aspirated from healthy donors and mononuclear cells were separated by Percoll lymphocytes separation liquid (1.073 g/ml) with centrifugation, cells were cultured in DMEM/F12 (1:1) medium containing 10% fetal bovine serum (FBS), 20 ng/ml epidermal growth factor (EGF) and 20 ng/ml basic fibroblast growth factor (bFGF).
[Stromal cells from human Wharton's jelly differentiate into neural cells].
Sichuan Da Xue Xue Bao Yi Xue Ban
January 2005
Objective: To investigate the neural differentiating capability of the umbilical cord tissue-derived stromal cells (UCSCs) in the attempt to find a new cell source for neural transplantation.
Methods: UCSCs from umbilical cord of human were cultured with tissue piece method, passaged by trypsin digestion. And Salvia miltiorrhiza was used to induce the cells to differentiate.
Objective: To investigate the culture method of skin-derived precursors (SKPs) and to explore a new cell source for cell transplantation of central nervous system.
Methods: Cells from skins of juvenile and adult mice were isolated and cultured in serum-free medium. A mechanical method was chosen to passage these cells and they were identified by the immunocytochemistry assay.
[Adipose tissue-derived stromal cells as vector for gene therapy in central nervous system].
Sichuan Da Xue Xue Bao Yi Xue Ban
July 2004
Objective: To investigate exogenous gene expressing ability of adipose tissue-derived stromal cell (ADSCs) and cell distribution after they were transplanted into brains, and to get the genetically modified cells for autografting.
Methods: ADSCs were transfected by Ad5beta gal adenovirus containing a report gene, LacZ gene, then they were transplanted into the adult brain of rats, or ADSCs labeled by Hoechst33258 were transplanted into the adult brain of rats to investigate the migration and distribution of cells.
Results: ADSCs showed a good expression of LacZ with X-gal staining after transfecting and transplantation into adult brains, and they could incorporate into the host brain tissues and no disruption was observed.
The in vitro myelin formation in neurospheres of human neural stem cells.
Chin J Traumatol
December 2003
Objective: To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres.
Methods: The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells.