A Prediction Model for Clinical Outcomes of COVID-19 Hospitalized Patients: Construction and Accuracy Assessment.

Background: The coronavirus disease 2019 (COVID-19) pandemic spread rapidly with considerable morbidity nationwide since China's liberalization in December 2022. Our work has focused on identifying different predictive factors from the laboratory examination of critically ill patients, and forecasting the unfavorable outcome of critically ill patients with COVID-19 through a combined diagnosis of biological markers.

Methods: We conducted a retrospective study at the Department of First Affiliated Hospital of Wenzhou Medical University, China, from December 24, 2022, to January 10, 2023, where 434 critically ill patients who met the inclusion criteria were involved.

Roles of MT-ND1 in Cancer.

The energy shift toward glycolysis is one of the hallmarks of cancer. Complex I is a vital enzyme complex necessary for oxidative phosphorylation. The mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1 (MT-ND1) is the largest subunit coded by mitochondria of complex I.

Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4'-di--glycosyltransferase from var. .

A highly efficient and promiscuous 7,4'-di--glycosyltransferase ZjOGT3 was discovered from the medicinal plant var. . ZjOGT3 could sequentially catalyse 4'- and 7--glycosylation of flavones to produce 7,4'-di--glycosides with obvious regio-selectivity.

GuRhaGT, a highly specific saponin 2''--rhamnosyltransferase from .

A highly regio- and donor-specific 2''--rhamnosyltransferase GuRhaGT was characterised from the medicinal plant . GuRhaGT could efficiently catalyse rhamnosylation at 2''-OH of the C-3 glycosyl moiety of triterpenoid saponins.

[Clinical study of lymphadenectomy of lung cancer by video-assisted thoracic small incision surgery].

Objective: To study the clinical efficacy of lymphadenectomy of lung cancer by video-assisted thoracic small incision surgery.

Methods: Radical lobectomy or pneumonectomy and systemic lymphadenectomy were performed in 197 patients with NSCLC through video-assisted thoracoscopic surgery. Pathological type and grade, total number of dissected lymph nodes, number of metastatic lymph nodes, survival and life quality were evaluated.

[Protein array technology applied in high throughput monoclonal antibody generation].

Unlabelled: To reduce the huge labor-cost in the screening in traditional monoclonal antibody generation, We established a new system for monoclonal antibody generation integrating with protein array. BALB/c mice were immunized by eight recombinant proteins respectively, and the positive hybridoma cells were obtained by cell fusion and ELISA screening. All the eight kinds of positive hybridoma cells were mixed, cloned, screened by protein array, and definite dilution cloned.