[Research of the electrophysiological properties of acutely dissociated spiral ganglion neurons isolated from mice using patch clamp technique].

Objective: To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice.

Methods: Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique.

Results: In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents.

[Establishment of methods of mechanical dissociation and culture of spiral ganglion neurons in postnatal mice].

Objective: To conduct a study on the methods for establishing a cytological model of mechanical dissociation and culture of spiral ganglion neurons (SGNs) in postnatal mice.

Methods: The spiral ganglion neurons were taken from 1-6-day old mice for primary culture. The morphology and the process of axon were observed by use of an inverted/phase contrast microscope.