Gold-nanostructured immunosensor for the electrochemical sensing of biotin based on liposomal competitive assay.

This work describes the development of a cost-effective, easy-to-use, portable immunoanalytical platform technology with sufficient sensitivity for use in the detection of physiologically important targets. Biotin, also known as vitamin H, was selected as the model analyte. The detecting system employs biotin-tagged, potassium ferrocyanide-encapsulated liposomes as the signal amplifier and PAH (poly allylamine hydrochloride)-modified, nanosized-Au particles assembled screen-printed electrode (nanoAu-SPE) as the working electrode.