The long-term consequences of Corona Virus Disease 2019 patients receiving Chinese herbal medicine treatments in acute phase: A protocol for systematic review and meta-analysis.

Background: In December 2019, the first case of Corona Virus Disease 2019 (COVID-19) associated with severe acute respiratory syndrome coronavirus-2 viral infection was described in Wuhan. Similar to SARS in 2003, COVID-19 also had a lasting impact. Approximately 76% of patients discharged after hospitalization for COVID-19 had neurological manifestations which could persist for 6 months, and some long-term consequences such as the gradual loss of lung function due to pulmonary interstitial fibrosis could have comprehensive effects on daily quality of life for people who were initially believed to have recovered from COVID-19.

Alpha7 nAChR Expression Is Correlated with Arthritis Development and Inhibited by Sinomenine in Adjuvant-Induced Arthritic Rats.

Sinomenine (SIN) is the active ingredient of the Chinese herb that has been used to treat rheumatoid arthritis (RA) for about 30 years in China. Marked expression of the alpha7 nicotinic acetylcholine receptor (7nAChR) in the joint synovium of RA patients suggested a relationship between 7nAChR and RA. This study investigated the relationship between 7nAChR and RA development and the effects of SIN on 7nAChR expression and .

The correlation between interleukin-34 and bone erosion under ultrasound in rheumatoid arthritis.

Rheumatoid arthritis (RA) is a chronic inflammatory arthropathy characterized by excessive synovial hyperplasia and progressive joint destruction. Pro-inflammatory cytokines play major roles in the regulation of synovial inflammation. The contribution of interleukin-34 (IL-34) in RA pathogenesis has been strongly suggested in clinical studies.

[Effects of Triptolide Combined with Homoharringtonine on Proliferation and Apoptosis of KG-1α Cells].

Objective: To investigate the effect and possible mechanism of low concentration of triptolide (TPL) combined with homoharringtonine (HHT) on the proliferation and apoptosis of KG-1α cells.

Methods: CCK-8 method was used to detect the antiproliferating effects of different concentrations of TPL and HHT single-use and combined use on KG-1α cells, and the combined index (CI) was calculated. The colony formation ability was also determined by methylcellulose colony formation assay, cell surface molecules, apoptosis rate and cell cycle changes were detected by flow cytometry.

Double substituted variant of Bacillus amyloliquefaciens esterase with enhanced enantioselectivity and high activity towards 1-(3',4'-methylenedioxyphenyl)ethyl acetate.

Bacillus amyloliquefaciens esterase (BAE) was applied to produce (R)-1-(3',4'-methylenedioxyphenyl)ethanol, a chiral drug intermediate. In this study, we improved the enantioselectivity of BAE by protein engineering instead of process engineering as used in our previous work. Saturation mutagenesis was carried out on eight positions of BAE based on structure modeling and substrate docking.

Effect of intestinal autochthonous probiotics isolated from the gut of sea cucumber (Apostichopus japonicus) on immune response and growth of A. japonicus.

The study isolated 224 bacteria from the intestine of Apostichopus japonicus, then selected and identified three of the bacteria (HS1, HS7, and HS10) which demonstrated amylase, lipase, and protease production capacity as candidate probiotics for sea cucumbers. The three potential probiotics showed no pathogenicity both in hemolytic assays on sheep blood agar plates and after immersing sea cucumbers in a suspension of the bacteria. To reveal the effects of these three potential probiotics on the innate immunity of sea cucumbers, total coelomocyte counts, respiratory burst activity, superoxide dismutase activity, lysozyme activity, acid phosphatase activity, and phagocytic activity by coelomocytes were examined after feeding with four different diets for up to 28 days.

A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: catalytic properties and performance in kinetic resolution of α-hydroxy acids.

A novel esterase, rPPE01, from Pseudomonas putida ECU1011 was heterologously expressed in Escherichia coli and identified for enzymatic resolution of hydroxy acids via O-deacetylation. α-Acetoxy carboxylates were converted with approximately 50% yield and excellent enantioselectivity (E>200) at a substrate concentration of 100 mM. The half-lives of rPPE01 were 14 days at 50°C and 30 days at 30°C, indicating the enzyme has relatively high thermostability.

A multicomponent synthetic strategy for two-carbon-tethered 1,3-oxathiole-indole pairs.

An efficient methodology for the multicomponent synthesis of new and highly functionalized heterocycles containing 1,3-oxathiole and indole units which are connected through an sp(2)-C(2) bridge has been developed. This domino reaction enables successful assembly of three new sigma bonds including a C-S bond and a C-O bond in a one-pot operation. Features of this strategy include mild conditions, convenient one-pot operation, and high stereo- and regioselectivity.

The processes leading to deception: ERP spatiotemporal principal component analysis and source analysis.

The cognitive and neural mechanisms leading to deception were studied by the event-related brain potential (ERP) technique. In a simulated deception situation with graded monetary incentives, participants made a decision to lie or be truthful in each trial and held their response until a delayed imperative signal was presented. Spatiotemporal principal component analysis (PCA) and source analysis revealed that brain activities dominant in the left lateral frontal area approximately 800-1,000 ms post-stimulus and over the central-frontal-parietal and right frontal areas after 1,300 ms were significantly more negative in the deceptive condition than in the truthful condition.