Seasonal resource selection of free-ranging Zhongwei goats in the semi-arid grassland.

Goats rarely move and forage randomly. They tend to move in ways generally influenced by biotic and abiotic factors, respectively. However, few studies have explored the foraging behaviour of goats in the absence of predation and human disturbance.

The Accelerated Progression of Atherosclerosis Correlates with Decreased miR-33a and miR-21 and Increased miR-122 and miR-3064-5p in Circulation and the Liver of ApoE-/- Mice with Streptozocin (STZ)-Induced Type 2 Diabetes.

Atherosclerosis is a major risk factor for type 2 diabetes (T2D) mortality. We aim to investigate the changes in miR-21, miR-122, miR-33a and miR-3064-5p in circulation and the liver of ApoE-/- mice with streptozocin (STZ)-induced T2D. Twenty 5-week-old male ApoE-/- mice were randomly assigned to the control (n = 10) and T2D group (n = 10) and intraperitoneally injected with a citrate buffer and streptozotocin (STZ) (40 mg/kg BW) once a day for three consecutive days.

Novel combined method of biosorption and chemical precipitation for recovery of Pb from wastewater.

A novel combined biosorption-precipitation process has been designed and applied to recycle Pb from low concentration lead containing wastewater. Pb was firstly removed selectively from wastewater by pyromellitic dianhydride (PMDA) modified sugarcane bagasse (SB) fixed-bed column, and then, it was desorbed into the concentrated eluate and recycled by adding chemical precipitant. Adsorption performance of the column and optimum desorption and precipitation condition for Pb were investigated in detail.

Facile synthesis of α-alkoxyl amides scandium-catalyzed oxidative reaction between ynamides and alcohols.

A novel and efficient scandium-catalyzed oxidative reaction between ynamides and alcohols for the facile synthesis of various α-alkoxyl amides is reported in this paper. The reaction avoids the need for the use of α-diazo carbonyls which are unstable and may cause some safety concerns. Instead, by using alkynes as the starting materials, this protocol features readily available substrates, compatibility with a broad range of functional groups, simple procedure, mild reaction conditions, and high chemoselectivity.

Selective adsorption and recycle of Cu from aqueous solution by modified sugarcane bagasse under dynamic condition.

Tetraethylenepentamine modified sugarcane bagasse was prepared and applied to test its feasibility in removing and recovering Cu from wastewater under dynamic condition. Results showed that the Cu could be selectively absorbed from wastewater by the modified SCB fixed bed column. To understand the adsorption mechanism, Cd had been selected as the model interfering ion to investigate how co-ions influence the adsorption of Cu on the sorbent.

[Construction of RevS gene knock-out mutant of Streptococcus suis serotype 2].

Objective: To construct a gene knock-out mutant of response regulator named RevS in Streptococcus suis serotype 2 virulent strain 05ZYH33, and to investigate the effects of its deletion on the biological characters of this pathogen and the pathogenesis to mice and piglets.

Methods: Recombinant gene knock-out vector consisting of Spc(r) cassette was constructed and flanking was constructed consisting of Spc(r) cassette with flanking homology regions to the RevS genes while the isogenic RevS-deficient mutant was screened by allelic replacement. The effects of RevS deletion on the basic biological characters of 05ZYH33 including growth stability, colonial morphology, haemolysis, Gram staining, growth curve and protein expression were examined in vitro.

[Study on molecular epidemiology of major pathogenic Streptococcus suis serotypes in middle part of Jiangsu province].

Objective: To determine the prevalence of Streptococcus suis and major pathogenic serotypes in middle part of Jiangsu province.

Methods: Tonsillar specimens from 303 slaughtered pigs aged 6 to 8 months were investigated for the presence of Streptococcus suis and major pathogenic serotypes by polymerase chain reaction (PCR) method. Bacteriological examination compared with molecular genetics identification for three Streptococcus suis isolates were also done.

[Study on expression of Streptococcus suis serotype 2 sly gene, purification and activity of its product].

Objective: To clone and express Streptococcus suis serotype 2 (S. suis 2) sly gene for constructing an foundation on identification of S. suis 2 protective antigen.

[Homologous analysis of Streptococcus suis srt gene and prokaryotic expression of srtA].

Aim: To elucidate the distribution of srt gene in the genome of S.suis 05ZYH33, prokaryotically express Sortase A and analyze its antigenicity.

Methods: Homologous genes encoding sortase family members were analyzed, and then the srtA gene of S.

Early-phase endotoxic shock-induced myocardial injury increases iNOS and selectin expression in macaque primate.

Background: Our previous study has established a macaque model with early-phase endotoxic shock. The present study further investigated myocardial and blood vessel injury in Macaques by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response to LPS treatment.

Methods: In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised into: an En group (n=6), receiving a dose of 2.

Effect of fluid resuscitation on ryanodine receptor in macaques with endotoxic shock.

Objective: Our recent study demonstrated that sodium bicarbonate improved cardiac function in macaque models with early-phase endotoxic shock. In the present study, we investigated further the ryanodine receptor/calcium release-channel (RyR) and calcium pump after fluid resuscitation of macaques with early-phase endotoxic shock.

Methods: Twenty-four anaesthetised macaques were assigned to four groups.

[Cloning and prokaryotic expression of truncated muramidase-released protein gene of human Streptococcus suis type 2].

Aim: To clone and express the truncated Habb mrp gene of human Streptococcus suis type 2 (S.suis 2) and detect its activity.

Methods: A pair of primers based on S.

[Detection of virulence-associated factors of Streptococcus suis by multiplex PCR assay].

Objective: To rapidly and sensitively detect the four virulence-associated factors of Streptococcus suis, a multiplex PCR was developed.

Methods: In the process of this reaction, four distinct DNA targets were amplified. One target was based on the serotype 2 (and 1/2) specific cps gene and the others were based on Streptococcus suis mrp, epf (epf*) and sly gene, encoding the MRP, EF(EF*) and Sly proteins of Streptococcus suis.

[Antimicrobial susceptibility in vitro of 32 drugs against pig Streptococcus isolates in China].

A number of 65 Streptococcus species isolated from diseased pigs were surveyed for in vitro minimal inhibitory concentration (MIC) to 32 drugs by microdilution test. Clinical categorization of isolates as susceptible or resistant was based on MIC interpretative standards of the National Committee for Clinical Laboratory Standards(NCCLS). None of the strains were susceptible to all antimicrobial compounds tested and most strains (96.

Endotoxic shock model with fluid resuscitation in Macaca mulatta.

These studies established a macaque model of early-phase endotoxic shock, and investigated the resuscitation effects of three different solutions. Twenty-four macaques were assigned to four groups. Nineteen animals were given an intravenous dose of 2.

[Screening and identification of mimic epitopes of monoclonal antibodies against hantaan virus using phage display technique].

Aim: To obtain mimic epitopes of monoclonal antibody (mAb) against Hantaan virus, and identify preliminarily their immunological characteristics.

Methods: Group-specific mAbs F3 and B11 were used as selective molecules for biopanning. After biopanning, the positive phages were identified by ELISA and DNA sequencing.