[Serum triacylglycerol-lowering effect of soybean beta-conglycinin in hyperlipidemic women].

Objective: Improving effects on serum triacylglyceride in women with hypertriacylglycerolemia by soybean beta-conglycinin.

Methods: A placebo-controlled, randomized, double-blind study was conducted in 100 women aged 40-60 with TC > 6.22 mmol/L (or TG > 1.

[Safety assessment of milk on reproductive development by reproduction study].

Objective: Observe the influence of whole fat milk and high-dose soy isoflavones (SI, 1 g/kg) on reproductive development to assess the safety of milk.

Methods: Sixty Spraque-Dawley rats (30 male and 30 female) were randomly separated into milk group, S1 group and control group (10 male and female each) according to body weight and were fed with milk, the diet with 1 g/kg SI and basic diet, respectively. Intervention measures were implemented from 11 weeks before mating to sexual maturity of F1.

Characterization and robust classification of EEG signal from image RSVP events with independent time-frequency features.

Unlabelled: This paper considers the problem of automatic characterization and detection of target images in a rapid serial visual presentation (RSVP) task based on EEG data. A novel method that aims to identify single-trial event-related potentials (ERPs) in time-frequency is proposed, and a robust classifier with feature clustering is developed to better utilize the correlated ERP features. The method is applied to EEG recordings of a RSVP experiment with multiple sessions and subjects.

Intravenous administration of multi-walled carbon nanotubes affects the formation of atherosclerosis in Sprague-Dawley rats.

Background: Carbon nanotubes (CNTs) have many potential applications, including as delivery systems for a variety of diagnostic or therapeutic agents. However, it has been suggested that exposure to carbon nano-materials may be a risk for the development of vascular diseases due to its impact on the vascular endothelium.

Materials And Methods: Male Sprague-Dawley rats (180-200 g) were used to generate an atherosclerosis (AS) model, and the effect of intravenous administration of multi-walled carbon nanotubes (MWCNTs) on AS was studied.

Sugary beverage intakes and obesity prevalence among junior high school students in Beijing - a cross-sectional research on SSBs intake.

Background: Excessive consumption of sugar-sweetened beverages (SSBs) may increase the risk of obesity. Data in regards to the consumption of SSBs is insufficient in the Chinese population, especially in middle school students experiencing rapid nutritional transition. We aimed to describe the consumption of SSBs among junior high school students and explore the relationship between SSB intake and adolescents' overweight/obesity in Beijing.

Changes of serum biochemical parameters and liver pathology in 18 patients with 1,1,2,2-tetrachloroethane-induced hepatotoxicity.

Objective: To evaluate the changes of serum biochemical parameters and liver pathology in patients with 1,1,2,2-tetrachloroethane (TTCE)-induced hepatotoxicity.

Methods: A total of 18 patients were diagnosed as TTCE-induced hepatotoxicity with an incubation period of 11-56 days. Blood samples were obtained from all patients and liver biopsy was performed in 16.

[Oxidative damage and immunotoxicity effect of people who exposed to electronic waste].

Objective: To investigate the relationship between risk factors and the effects of antioxidation and immune function in adults who exposed to electronic waste( e-waste).

Methods: The exposed group was chosen from the people who lived in the e-waste disposing areas of Zhejiang province. The control group was chosen from people who lived in unpolluted area.

Synthesis of acridines and persubstituted phenols from cyclobutenones and active methylene ketones.

A new benzannulation strategy that proceeds via a regiospecific [4 + 2] cycloaddition of readily available cyclobutenones and active methylene ketones has been developed. On the basis of this strategy, persubstituted phenols/anilines with up to six different functional groups on the benzene ring were synthesized in a single step. In addition, a series of acridine derivatives were prepared in excellent yield from persubstituted phenols/anilines.

Health risk assessment of lead for children in tinfoil manufacturing and e-waste recycling areas of Zhejiang Province, China.

Tinfoil manufacturing and electronic waste (e-waste) recycling remain rudimentary processes in Zhejing Province, China, which could account for elevated blood lead levels (BLLs) and health impacts on children. We assessed the potential health risks of lead in tinfoil manufacturing and e-waste recycling areas. 329 children in total aged 11-12 who lived in a tinfoil manufacturing area (Lanxi), an e-waste recycling area (Luqiao) and a reference area (Chun'an) were studied.

Direct and efficient cellular transformation of primary rat mesenchymal precursor cells by KSHV.

Infections by viruses are associated with approximately 12% of human cancer. Kaposi's sarcoma-associated herpesvirus (KSHV) is causally linked to several malignancies commonly found in AIDS patients. The mechanism of KSHV-induced oncogenesis remains elusive, due in part to the lack of an adequate experimental system for cellular transformation of primary cells.

A genome-wide inducible phenotypic screen identifies antisense RNA constructs silencing Escherichia coli essential genes.

Regulated antisense RNA (asRNA) expression has been employed successfully in Gram-positive bacteria for genome-wide essential gene identification and drug target determination. However, there have been no published reports describing the application of asRNA gene silencing for comprehensive analyses of essential genes in Gram-negative bacteria. In this study, we report the first genome-wide identification of asRNA constructs for essential genes in Escherichia coli.

Bayesian non-negative factor analysis for reconstructing transcription factor mediated regulatory networks.

Background: Transcriptional regulation by transcription factor (TF) controls the time and abundance of mRNA transcription. Due to the limitation of current proteomics technologies, large scale measurements of protein level activities of TFs is usually infeasible, making computational reconstruction of transcriptional regulatory network a difficult task.

Results: We proposed here a novel Bayesian non-negative factor model for TF mediated regulatory networks.

Biclustering of time series microarray data.

Clustering is a popular data exploration technique widely used in microarray data analysis. In this chapter, we review ideas and algorithms of bicluster and its applications in time series microarray analysis. We introduce first the concept and importance of biclustering and its different variations.

Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents.

The neuroimmunological and behavioral consequences of a high-fat diet (HFD) are not well delineated. This is especially true when short term (24 h) fasting is used as a physiologic stressor. In this study, we examined the impact of a HFD on learning and memory and depressive-like behaviors to understand how fasting impacts neuroimmunity and whether obesity modulates the response.

Exocytosis and mobility of histamine vesicles exhibit no difference to that of histamine-negative vesicles in guinea pig superior cervical ganglion neurons.

Our previous studies have shown that histamine existed widely in the sympathetic nervous system and functioned differentially on the sympathetic nerve activation level. Therefore, in this study, we tried to find out whether it is the special exocytosis/recycling of histamine-containing vesicles that contribute to those differential histamine synaptic effects. By using N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridinium dibromide and histamine immunostaining methods, we confirmed that histamine was stored in small vesicles and found that the histamine-containing vesicles included the recycling pool and the reserve pool.

Preparation and in vitro-in vivo evaluation of double layer coated and matrix sustained release pellet formulations of diclofenac potassium.

The purpose of the present study was to prepare matrix extended release pellets of diclofenac potassium using low amount of release-modifying agents and, to compare its performance in vivo with coated pellets and matrix tablets. Coated pellets were prepared by extrusion-spheronization, followed by double layer coating using different polymers separately. Matrix pellets with different release rate in vitro were prepared by extrusion-spheronization with different kinds of retarding materials.

Robust inference of the context specific structure and temporal dynamics of gene regulatory network.

Background: Response of cells to changing endogenous or exogenous conditions is governed by intricate molecular interactions, or regulatory networks. To lead to appropriate responses, regulatory network should be 1) context-specific, i.e.

Moving affinity boundary electrophoresis and its selective isolation of histidine in urine.

Affinity analysis is a key biotechnique used in the fields of biology and biomedicine. Herein, we advanced the concept of moving affinity boundary (MAB) using metal ion Ni(II) and histidine (His) as the model inorganic ion and ligand, respectively, developed the simple method of MAB affinity capillary electrophoresis (MAB-ACE), and carried out the relative experiments. The experiments manifested that (a) an MAB could be created with the model metal ion and ligand; (b) the MAB-ACE could specifically capture His rather than other amino acids, or numerous metabolites in human urine; and (c) the capture had the merits of simultaneous focusing and separation to the target metabolite of His.

Preparation of ergoloid mesylate submicron emulsions for enhancing nasal absorption and reducing nasal ciliotoxicity.

The aim of this investigation was to prepare ergoloid mesylate submicron emulsions (EMSEs) for enhancing nasal absorption of drug and reducing nasal ciliotoxicity. Following intranasal administrations of EMSE and ergoloid mesylate solution (EMS) and intravenous administration of EMS to rats separately at the dose of 2 mg kg(-1), the levels of EM in blood and the cerebrospinal fluid (CSF) were evaluated by microdialysis method. The nasal ciliotoxicity was evaluated by using in situ toad palate model.

Enrichment constrained time-dependent clustering analysis for finding meaningful temporal transcription modules.

Motivation: Clustering is a popular data exploration technique widely used in microarray data analysis. When dealing with time-series data, most conventional clustering algorithms, however, either use one-way clustering methods, which fail to consider the heterogeneity of temporary domain, or use two-way clustering methods that do not take into account the time dependency between samples, thus producing less informative results. Furthermore, enrichment analysis is often performed independent of and after clustering and such practice, though capable of revealing biological significant clusters, cannot guide the clustering to produce biologically significant result.

Highly efficient access to bi- and tricyclic ketals through gold-catalyzed tandem reactions of 4-acyl-1,6-diynes.

Single step: Fused bicyclic and bridged tricyclic ketals were synthesized in a single step from the reactions of easily available 4-acyl-1,6-diynes with H(2)O and alkanols (see scheme). The highly efficient AuCl(3)-catalyzed multicomponent domino reactions, involving five C-O bond formations, can proceed in a highly regio- and diastereoselective manner at room temperature under air and lead to structures of high molecular complexity from simple starting materials in an atom economic way.

Immunoregulatory effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et. Gilg on immune functions of ICR mice.

Objective: To evaluate the effects of ethyl-acetate fraction (EAF) of extracts from Tetrastigma hemsleyanum Diels et. Gilg (TDG) on immune functions of ICR mice.

Methods: ICR mice were exposed to different doses of EAF for 15 or 30 days and then their immune functions were analyzed, including ConA-induced splenic lymphocyte transformation, SRBC-induced delayed type hypersensitivity response, serum hemolysin analysis, antibody-producing cells, peritoneal macrophage phagocytized chicken red blood cells, natural killer cell activity, and serum level of cytokines.

Renal protective activity of Hsian-tsao extracts in diabetic rats.

Objective: To investigate the renal protective activity of Hsian-tsao Mesona procumbens Hemsl. water extracts in diabetic rats.

Methods: Thirty Sprague-dawley female rats were randomly divided into three groups (n = 10 each), "control group" with intraperitoneal saline injection, "diabetic group" with 60 mg of intraperitoneal streptozotocin injection per kg of body weight and "Hsian-tsao group" with intragastric administration of Hsian-tsao extraction everyday for 4 weeks after intraperitoneal streptozotocin injection.

Histamine H(4) receptors regulate ACTH release in AtT-20 cells.

The early research described that adrenocorticotropic hormone (ACTH) release from mouse pituitary tumor AtT-20 cells was regulated by histamine H(3) receptors. Here, we provide the evidence that histamine H(4) receptor was expressed in AtT-20 cell, and that the accelerated ACTH secretions from the cells by histamine and R-alpha-methylhistamine were blocked by JNJ7777120, a specific H(4) receptor antagonist, in concentration dependent manner, but not by the H(1) and H(2) receptor antagonists. The results indicate, for the first time, that histamine H(4) receptor, rather than histamine H(3) receptor, played a role in regulation of ACTH release from mouse pituitary AtT-20 cells.