Ascaris spp. and Capillaria caudinflata infections in captive-bred crested ibis (Nipponia nippon) in China.

Crested ibis (Nipponia nippon), an endan gered native bird, was called the "precious stone" of oriental birds. N. nippon was considered a critically endangered species in the IUCN Red List of Threatened Species and a first-class national protected animal in China.

Encystation--survival of Blastocystis hominis in immunocompetent mice abdomen cavity.

Human Blastocystis hominis were isolated from diarrhea patients' feces and cultured in vitro. Then the cultures were inoculated intraperitoneally to laboratory mice. The B.

Broad profiling of DNA-binding transcription factor activities improves regulatory network construction in adult mouse tissues.

Molecular systematics involves the description of the regulatory networks formed by the interconnections between active transcription factors and their target expressed genes. Here, we have determined the activities of 200 different transcription factors in six mouse tissues using an advanced mouse oligonucleotide array-based transcription factor assay (MOUSE OATFA). The transcription factor signatures from MOUSE OATFA were combined with public mRNA expression profiles to construct experimental transcriptional regulatory networks in each tissue.

Novel high-throughput profiling of human transcription factors and its use for systematic pathway mapping.

Transcription factors (TFs) are crucial components of regulatory networks that control gene transcription. Current TF assays are limited to the analysis of a single TF or require TF-specific antibodies. Here we report the Single Primer Amplification assisted Oligonucleotide Array-based Transcription Factor Assay (SPA-OATFA) which can directly analyze the binding activities of 240 human TFs simultaneously.

[Vitro culture of blastocystis hominis in medium DMEM].

Objective: To establish the vitro culture of Blastocystis hominis (B. h) in medium DMEM for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza.

Methods: The growth, reproduction and relevant factors of B .

[Study on the biological characteristic of Blastocystis hominis: morphology, mode of reproduction and the relation to bacteria].

Objective: To observe the reproductive modes of Blastocystis hominis and study the relation between this protozoa and bacteria.

Methods: Using the Iodine and Haematoxylin staining, the morphology of B. h from patients and RPMI 1640 medium were observed.

[Experimental infection of mice with Blastocystis hominis].

Objective: To seek a better pathway and proper number of parasites for Blastocystis hominis (B.h) infection in normal and immunocompromised ICR mice.

Methods: (1) 10(4), 10(5) and 10(6) B.

Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data.

[Study on morphology of Blastocystis hominis in culture and from diarrhea patients].

Objective: To study morphology of different stage Blastocystis hominis (B. h) for establishing a base in the research of life cycle and pathogenicity of B. h and providing information for clinical laboratory.