[Real-time fluorescent quantitative PCR for detection of peripheral blood T-cell receptor excision circles].

Objective: To develop and optimize real-time fluorescence quantitative PCR (FQ-PCR) with the housekeeping gene RAG2 as cell number control to quantify T-cell receptor excision circle (TREC) in the peripheral blood.

Methods: The real-time PCR system for amplifying TREC and RAG2 genes was established on the basis of ABI 7000 apparatus using Golden Taq system, designed primers, TaqMan-MGB probes and optimized buffer. PCR conditions were optimized with standard samples of TREC plasmid.

[Changes of peripheral blood CD45RA+ cells after hematopoietic stem cell transplantation: evaluation with four-color flow cytometry].

Objective: To investigate the alteration of peripheral blood naive T cells in adult patients after hematopoietic stem cell transplantation (HSCT).

Methods: Peripheral blood samples were collected from 49 patients requiring stem cell transplantation before and 15, 30, 90, and 180 d after transplantation, respectively. Dynamic changes of CD4(+)CD45RA(+)/CD8(+)CD45RA(+) cell counting were measured using flow cytometry.