[Characterization of the HIV-1 subunit vaccine containing the gp41 NHR domain of the HIV-1 CRF01_AE recombinant subtype in China].

Aim: To design and construct an HIV-1 subunit vaccine containing the N-terminal heptad repeat (NHR) domain N51 in gp41 of the HIV-1 CRF01_AE recombinant subtype in China and study its immunogenicity.

Methods: Two pairs of primers were designed to amplify DNA fragment encoding N51Fd gene, which was then subcloned into pFUSE-hIgG1-Fc2 vector. The recombinant plasmid pFUSE/N51Fd was confirmed by DNA sequencing.