Potential role of Jun activation domain-binding protein 1 and phosphorylated p27 expression in prognosis of glioma.

To investigate whether Jun activation domain-binding protein 1 (Jab1) expression is correlated with p27 protein and its phosphorylation status as well as how it might be clinically relevant in glioma, we carried out an immunohistochemical study of Jab1, Ser10-phosphorylated p27 (pSer10p27), and p27 using biopsies from 192 patients with primary glioma. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of patients. Immunostaining revealed a positive correlation between Jab1 and cytoplasmic p27 as well as pSer10p27 in all glioma cases.

Reduced expression of SMAD4 in gliomas correlates with progression and survival of patients.

Background: To examine the expression of SMAD4 at gene and protein levels in glioma samples with different WHO grades and its association with survival.

Methods: Two hundreds fifty-two glioma specimens and 42 normal control tissues were collected. Immunochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of SMAD4.