A regio-specific 4--methyltransferase from regiospecifically catalyzing 8-prenylkeampferol to icaritin.

is widely used in traditional Chinese medicine and contains rich bioactive compounds. These compounds often have a methyl group at their 4'-OH position catalyzed by methyltransferases. Therefore, studying methyltransferases in plants is of great significance.

Exploring the catalytic diversity of two short-chain dehydrogenases/reductases from .

Short-chain dehydrogenase/reductases (SDRs) belong to the NAD(P)(H)-dependent oxidoreductase superfamily, which have various functions of catalyzing oxidation/reduction reactions and have been generally used as powerful biocatalysts in the production of pharmaceuticals. In this study, ScSDR1 and ScSDR2, two new SDRs have been identified and characterized from 3.5365.

Enzymatic synthesis of anhydroicaritin, baohuoside and icariin.

Anhydroicaritin (), baohuoside () and icariin () were recognized as major pharmacologically active ingredients of plants. Their primary means of acquisition were chemical isolation from plants. However, it suffers from low yield, environmental pollution and shortage of plants.

Two new 5/6/6 polyketide-amino acid hybrids from a genetic mutant of sp. F-31.

Peridecalins C and D ( and ), one decalin and one oxygen-decalin containing polyketide-amino acid hybrids with 5/6/6 ring system, was isolated from a genetic mutant of sp. F-31. Their structures were elucidated through extensive spectroscopic data analysis, including 1 D/2D NMR and HR-MS spectra.

Sesquiterpenes from the endophytic fungus sp. F-31.

One new eremophilane sesquiterpene periconianone L (), together with four known guaiane-type sesquiterpenes 4,10,11-trihydroxyguaiane (), (-)-guai-1(10)-ene-4,11-diolhydroxymecuration (), guaidiol A (), and epi-guaidiol A () were isolated from the endophytic fungus sp. F-31. The structure of the new compound was established by spectroscopic methods, including UV, IR, HRESIMS, and extensive NMR techniques.

[Study on chemical bibenzyls in Dendrobium gratiosissimum].

Nineteen compounds were isolated and structurally characterized from an ethanol extract of Dendrobium gratiossimum, including dendrogratiol A(1), DDB-1(2), 3,4-dihydroxyl-5,3',4'-trimethoxybibenzyl(3), amoenylin(4), chrysotoxine(5), DTB(6), 3,4,4'-trihydroxyl-5,3'-dimethoxybenzyl(7), 3-methylgiga(8), aloifol(9), gigantol tetramethyl ether(10), batatasin Ⅲ(11), moscatilin(12), moniliformine(13), gigantol(14), DMB(15), flavanthrinin(16), cannithrene-2(17), 3,4-dihydroxyl-5,4'-dimethoxystilbene(18) and 4-hydroxy-3,5,4'-trimethoxystilbene(19). 1 was a new compound, and 2-10, 16, 18 and 19 were obtained from this plant species for the first time. In vitro cytotoxic and antiviral activities of these isolates were evaluated, which displayed that 4 showed moderate cytotoxicity against human hepatoma cell line HepG2 with the IC_(50) of 10.

Biosynthesis of polyketides by two type III polyketide synthases from .

Various bioactive polyketides have been found in However, the polyketide synthases (PKSs), which participate in biosynthesis of polyketides in remain unknown. In this study, two type III PKSs (AbPKS1 and AbPKS2) were identified from . AbPKS1 and AbPKS2 were able to utilize malonyl-CoA to yield heptaketides (TW93a and aloesone) and octaketides (SEK4 and SEK4b), respectively.

Bistachybotrysin K, one new phenylspirodrimane dimer from with potent cytotoxic activity.

Bistachybotrysin K (), one new phenylspirodrimane dimer with a central 6/7 oxygen heterocycle core, was isolated from the fungus CGMCC 3.5365. Its structure was elucidated by extensive spectroscopic data and single-crystal X-ray diffraction.

Three new phenylspirodrimane derivatives with inhibitory effect towards potassium channel Kv1.3 from the fungus .

Three new phenylspirodrimanes derivatives named stachybotrysins H and I ( and ) and stachybotrin E (), together with one known compound stachybotrylactam (), were isolated from CGMCC 3.5365. Their structures were determined by extensive NMR data and mass spectroscopic analysis.

Enzymatic synthesis of glucuronidated metabolites of two neurological active agents using plant glucuronosyltransferases.

Glucuronidation is an important and popular metabolic reaction in vivo of drugs. The further evaluation of biological activity and toxicity of glucuronides is necessary in the course of the drug research and development. However, the synthesis of glucuronides is limited by the lack of efficient approach.

Lanostane triterpenoids and ergostane-type steroids from the cultured mycelia of Ganoderma capense.

Two new lanostane triterpenoids (1 and 2), two new ergostane-type steroids (3 and 4) together with two known lanostane triterpenoids (5 and 6) and one known steroid (7) were isolated from the cultured mycelia of Ganoderma capense (CGMCC 5.71). Their structures were determined on the basis of extensive spectroscopic (HRESIMS, 1D NMR, 2D NMR) data analyses.

Four new monoterpenoids from an endophytic fungus Periconia sp. F-31.

Five monoterpenoids were isolated from the endophytic fungus Periconia sp. F-31, including three new carene-type monoterpenoids, 2-carene-5,8-diol (1), 2-carene-8,10-diol (2), 2-carene-8-acetamide (3), one new menthene-type monoterpenoid 8-hydroxy-1,7-expoxy-2-menthene (4), and one known monoterpenoid anethofuran (5). The structures of all compounds were elucidated based on a comprehensive spectroscopic data analysis, electronic circular dichroism (ECD), and calculated ECD.

Bioactive steroids and sorbicillinoids isolated from the endophytic fungus Trichoderma sp. Xy24.

A new steroid glucoside (1), along with nine known steroids (2-10) and four known sorbicillinoids (11-14), were isolated from the endophytic fungus Trichoderma sp. Xy24. Their structures were elucidated on the basis of spectroscopic data analyses and by comparison with reported data.

Neural anti-inflammatory sesquiterpenoids from the endophytic fungus Trichoderma sp. Xy24.

Three new sesquiterpenoids trichoacorenols B-C and cyclonerodiol B (1-3), along with three known ones (4-6), were isolated from the mangrove plant endophytic fungus Trichoderma sp. Xy24 using various column chromatography techniques. The structures of these compounds were determined on the basis of extensive spectroscopic data analyses.

[A new lactone derivative from plant endophytic fungus Periconia sp. F-31].

To investigate the secondary metabolites of endophytic fungi Pericinia sp. F-31. Column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds.

Flavonoids from the cultured cells of Glycyrrhiza uralensis.

Two new flavonoids (1 and 2), along with 14 known ones (3-16), were isolated from the cultured cells of Glycyrrhiza uralensis. Most of them were prenylated flavonoids. Their structures were elucidated on the basis of spectroscopic data analysis.

[Isolation and Sequence Analysis of the Xa21 Gene Wxon II Homologus from Different Species of Wild Rice in Yunnan.].

The Xa21 gene previously cloned from the wild rice species Oryzae longistaminata confers broad-spectrum resistance to rice leaf blight caused by different strains of Xanthomonas oryzae pv. oryzae. Here we attempted to determine the existence of Xa21 homologs in other wild rice species and rice cultivars and the sequence differences between the homologs.