Publications by authors named "Ji-da Xu"
Article Synopsis
- Identifying biomarkers for early cancer detection is crucial for improving diagnosis and treatment outcomes, specifically focusing on esophageal squamous cell carcinoma (ESCC) in this study.
- Researchers analyzed DNA methylation patterns in cancer cells and plasma cell-free DNA from early-stage ESCC patients to construct a reliable methylation profile.
- Seven specific CG sites showed significant hypermethylation in three genes (CASZ1, CDH13, and ING2), demonstrating 100% sensitivity and specificity for potential non-invasive diagnosis of esophageal cancer through blood samples.
Background & Objective: This study was conducted to investigate the altered gene expression of MCF-7 cell before and after the treatment with beta-carotene using cDNA microarray and to investigate the mechanism which beta-carotene induce breast cancer cell apoptosis.
Methods: Two fluorescence cDNA probes were made using reverse transcriptional reaction from mRNA of beta-carotene untreated or treated MCF-7 cells (human estrogen receptor positive breast cancer cells), marked with two different fluorescence dyes (cy3 and cy5) respectively, hybridized with expressed cDNA microarray scanned and analyzed by computer system and finally the expressed gene was produced.
Results: A total of 21 genes related to cell apoptosis, cell signal transduction, protein translation and immunity were expressed differently after the treatment of beta-carotene, which 3/21 were up-regulated (AF040958, AK001555,g41894),18/21 were down- regulated(hshsp90r,U83857,AB014509,AF126028,AF053641,AF117386,AF050127,NM_012177,humtopi,AJ250915,U37547,U78798,NM_004849,NM_005346,AF004711,NM_006595,NM_001418,AB015051).