Publications by authors named "Ji-Youn Chang"
Objective: This study aimed to investigate the effects of zinc compounds on the candidacidal activities of lysozyme and the peroxidase (PO) and glucose oxidase-mediated peroxidase (GO-PO) systems against Candida albicans.
Methods: Four zinc compounds were used: zinc chloride, gluconate, lactate, and sulphate. Three antimicrobial systems were used: hen egg-white lysozyme (HEWL), the PO system [bovine lactoperoxidase (bLPO), potassium thiocyanate, and hydrogen peroxide], and the GO-PO system (bLPO, potassium thiocyanate, glucose oxidase, and glucose).
Article Synopsis
- The study aimed to examine how common preservatives in oral health products impact the enzymatic activities of three enzymes: lysozyme, peroxidase, and α-amylase, both in solution and on the surface of hydroxyapatite beads.
- Four preservatives were tested: sodium benzoate, methylparaben, propylparaben, and benzalkonium chloride, using sources like hen egg-white lysozyme and human saliva for enzyme activity measurements.
- Results showed that parabens significantly decreased peroxidase and lysozyme activity in solution, while sodium benzoate and benzalkonium chloride were deemed better preservatives than parabens based on their effects on salivary enzymes.
Objectives: To investigate the clinical characteristics and salivary biomarkers in each type of burning mouth syndrome (BMS) patients.
Materials And Methods: Ninety-eight postmenopausal female patients with BMS were included. Fifty and 21 patients were assigned to the primary and secondary groups, respectively.
Article Synopsis
- - This study looked at how different zinc compounds (like zinc chloride and gluconate) affect the activities of key enzymes (lysozyme, peroxidase) and their ability to combat fungi, particularly Candida albicans.
- - Results showed that zinc gluconate and sulfate boosted lysozyme activity while all zinc compounds reduced peroxidase activity, with zinc binding to these enzymes in varying degrees.
- - The zinc compounds were effective against C. albicans, with a minimum inhibitory concentration of 1.0-2.4 mM and showing candidacidal activity ranging from 17.7-47.0%, suggesting they could be useful for improving oral health, especially in older adults.
Objectives: To investigate the viscosity values of mixtures of hyaluronic acids with different molecular weights and the effects of these mixtures on the enzymatic activities of lysozyme and peroxidase.
Materials And Methods: Mixtures of high molecular weight (1 or 2 MDa) and low molecular weight (10 or 100 kDa) hyaluronic acids at different concentrations were used for viscosity measurements. Hyaluronic acid mixtures showing viscosity values similar to those of human whole saliva were used for enzyme experiments in solution and on hydroxyapatite surface.
Salivary biomarkers of inflammation and oxidative stress in healthy adults.
Arch Oral Biol
January 2019
Objectives: Diagnostic value of saliva depends on the reproducibility of data in repeatedly collected samples and predictable correlations between saliva and blood. We aimed to investigate the reliability, blood reflectance, and influence of blood contamination in the analysis of inflammatory and oxidative stress biomarkers in saliva samples.
Design: In total, 37 healthy young male participants (26.
Article Synopsis
- - The study explored how the molecular weight of hyaluronic acid impacts its viscosity and the activities of the enzymes lysozyme and peroxidase, using different moles of hyaluronic acid and measuring viscosity at varying concentrations.
- - Results indicated that only the highest molecular weight (2 MDa) hyaluronic acid notably inhibited lysozyme activity in saliva, with overall inhibitory effects being more pronounced on the enzyme activities when hyaluronic acid was on a surface compared to in solution.
- - High-molecular-weight hyaluronic acids at low concentrations had viscosity values similar to human saliva, suggesting that their potential applications in oral health could be beneficial, particularly due to their effects on enzymatic activity.
Objectives: To investigate possible relationships among oral mucosal epithelial MUC1 expression, salivary female gonadal hormones and stress markers, and clinical characteristics in patients with burning mouth syndrome (BMS).
Design: Thirty post-menopausal female patients with BMS (60.0±5.
Polymorphisms of interleukin-1β and MUC7 genes in burning mouth syndrome.
Clin Oral Investig
April 2017
Objective: The objectives of the present study are to compare polymorphisms of the IL-1β and MUC7 genes between patients with burning mouth syndrome (BMS) and controls and to investigate relationships between these polymorphisms and clinical characteristics in BMS patients.
Materials And Methods: Forty female BMS patients and 40 gender- and age-matched controls were included. Genomic DNA was extracted from saliva samples.
Objective: To investigate whether xylitol and sorbitol affect enzymatic and candidacidal activities of lysozyme, the peroxidase system, and the glucose oxidase-mediated peroxidase system.
Design: Xylitol and sorbitol were added to hen egg-white lysozyme, bovine lactoperoxidase, glucose oxidase-mediated peroxidase, and whole saliva in solution and on hydroxyapatite surfaces. The enzymatic activities of lysozyme, peroxidase, and glucose oxidase-mediated peroxidase were determined by the turbidimetric method, the NbsSCN assay, and production of oxidized o-dianisidine, respectively.
Objective: To investigate interactions between hyaluronic acid (HA), lysozyme, and the glucose oxidase-mediated lactoperoxidase (GO-LPO) system in enzymatic and candidacidal activities.
Design: The influences of HA (0.5, 1.
Objective: Expression levels of MUC1 and TLR-2 were evaluated in burning mouth syndrome (BMS) patients and compared with those of controls and oral lichen planus (OLP) patients. The relationships between the expression levels of MUC1 and TLR-2 and levels of salivary pro-inflammatory cytokines were also investigated.
Design: Ten female BMS and ten female OLP patients were included.
Objective: To investigate the viscosity of yam tuber mucilage (YTM) and its effects on lysozyme and peroxidase activities in solution phase and on surface phase.
Methods: Two kinds of YTM were extracted, one containing both protein and carbohydrate and the other containing mainly carbohydrate. Hen egg-white lysozyme and bovine lactoperoxidase were used as lysozyme and peroxidase sources, respectively.
Objective: The purpose of this study was to investigate the candidacidal activity of the glucose oxidase-mediated lactoperoxidase system at various levels of glucose and glucose oxidase.
Design: Candida albicans ATCC strains 18804, 10231, 11006, bovine lactoperoxidase (25 and 50 μg/mL), and KSCN (1 mM) were used. Different levels of glucose oxidase (1, 5, 10, and 20 units/mL) and glucose (0.
Objective: MUC1 is primarily involved in the protection of epithelial surfaces. Decreases in oral mucosal defence can be a predisposing factor for the development of oral mucosal diseases in the elderly. The aim of this study was to compare MUC1 expression level in oral mucosal epithelial cells of the elderly with that of young adults.
View Article and Find Full Text PDFObjective: To investigate the effects of peroxidase or the peroxidase system on the enzymatic and candidacidal activities of lysozyme.
Design: The effects of peroxidase on lysozyme were examined by incubating hen egg-white lysozyme (HEWL) with bovine lactoperoxidase (bLPO). The influence of the peroxidase system on lysozyme was examined by the subsequent addition of potassium thiocyanate and hydrogen peroxide.
Objective: The purpose of this study was to investigate the viscosity and wettability of a water-soluble extract of yam and its effects on lysozyme and peroxidase activities.
Design: Human whole saliva, yam tuber, hen egg-white lysozyme, and bovine lactoperoxidase were used. Viscosity was measured with a cone-and-plate digital viscometer, while wettability was determined by measuring the contact angle.