The detection and clinical features of human cytomegalovirus infection in infants.

The aim is to investigate the spectrum of disease in 378 infants with human cytomegalovirus infection. In these patients, 27.78% were systemic infection and 72.

[Clinical characteristics and outcomes of respiratory distress syndrome in term and late-preterm neonates].

Objective: Respiratory distress syndrome (RDS) is a frequently seen acute respiratory disorder in the newborn infants. Since the original description of deficiency of the pulmonary surfactant in premature neonates by Avery in 1959, RDS has most commonly been attributed to developmental immaturity of surfactant production. But in clinical practice it has been found that there was RDS in term and late-term neonates.

Cytomegalovirus-associated idiopathic thrombocytopenic purpura in Chinese children.

The objective was to investigate the clinical features, glycoprotein B (gB) genotype and therapy of the human cytomegalovirus (CMV)-associated idiopathic thrombocytopenic purpura (ITP) in Chinese children. Clinical features and laboratory data of 25 CMV-associated ITP, including 18 males and 7 females with a median age of 0.3 y, were analysed and compared with 116 CMV-negative ITP.

[Association between clinical manifestations of infants with human cytomegalovirus infection and glycoprotein B genotype].

Objective: To investigate the association between the clinical manifestations of infants with human cytomegalovirus (HCMV) infection and glycoprotein B (gB) genotype.

Methods: Urine samples were obtained from 107 symptomatic infants with HCMV infection confirmed by fluorescence quantitative PCR, 70 male and 37 female, aged 5 d-8 months, and 25 asymptomatic infants with HCMV infection, 16 male and 9 female, aged 21 d-7 months. A fragment of glycoprotein B gene was amplified by nested PCR (nPCR).

Cytomegalovirus gB genotype and clinical features in Chinese infants with congenital infections.

Objective: To investigate cytomegalovirus (CMV) glycoprotein B (gB) genotypes and clinical features in Chinese infants with congenital infections.

Methods: Urine samples were obtained from 79 infants with human CMV infection confirmed by quantitative fluorescence polymerase chain reaction (PCR). A fragment of the gB gene was amplified by nested PCR.

[Effects of phytoestrogens on gap junctional intercellular communication].

Objective: To observe effects of phytoestrogens quercetin (QC), Genistein (GEN), coumestrol (COM), and enterolactone (ENL) on gap junctional intercellular communication (GJIC) in HaCaT cells.

Methods: HaCaT cells were exposed to QC, GEN, COM, and ENL at 0.1, 1.

[Application of flow cytometry to detect PP65 antigenemia for diagnosis and monitoring of human cytomegalovirus infection].

Objectives: To evaluate the clinical significance of flow cytometry (FCM) to detect the cytomegalovirus (CMV) PP65 antigen in patients with CMV infection.

Methods: Samples from 35 patients without CMV infection were used as negative control. The definite diagnosis of CMV infection was based on the national criteria for CMV infection.

[Relationship among serum cortisol and insulin and blood glucose in the asphyxiated neonates].

Objective: To explore the dynamic change of serum cortisol and insulin levels, and their relation with blood glucose concentration in asphyxiated neonates.

Methods: The levels of serum cortisol and insulin at d1,d3 and d7 of birth were measured by radioimmunoassay and the concentration of blood glucose was measured with glucose oxidase method in 43 asphyxiated neonates.

Results: The levels of serum cortisol at d 1, d 3 and d 7 of birth were gradually decreased (P<0.