Publications by authors named "Ji-Xiong Leng"
Article Synopsis
- Cancer antigen 125 (CA125) is a blood marker used to detect ovarian cancer, but its levels can also rise due to non-cancerous conditions, making diagnosis tricky.
- Researchers engineered cells to express CA125 that carries the Tn antigen, hoping to improve the accuracy of ovarian cancer diagnosis.
- They achieved this by knocking out specific genes in kidney cells to increase Tn antigen levels, using a system to anchor CA125 to the cell surface, and ultimately converting it to a form that can be secreted for better detection.
Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry. The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing. In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.
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