Dorsomorphin attenuates ABCG2-mediated multidrug resistance in colorectal cancer.

Colorectal cancer is a common malignant tumor with high mortality, for which chemotherapy resistance is one of the main reasons. The high expression of ABCG2 in the cancer cells and expulsion of anticancer drugs directly cause multidrug resistance (MDR). Therefore, the development of new ABCG2 inhibitors that block the active causes of MDR may provide a strategy for the treatment of colorectal cancer.

[Research progress of Wallis dynamic stabilization system for lumbar degenerative diseases].

Wallis dynamic stabilization system is a surgical approach in the non-fusion technique of lumbar spine, consisting of interspinous blockers and dacron artificial ligaments that provide stability to the spine while maintaining a degree of motion in the affected segment. Recent studies have demonstrated the significant benefits of Wallis dynamic stabilization system in treating lumbar degenerative diseases. It not only improves clinical symptoms, but also effectively delays complications such as adjacent segmental degeneration.

Induction immune-checkpoint inhibitors for resectable oncogene-mutant NSCLC: A multicenter pooled analysis.

Despite limited efficacy of immunotherapy for advanced non-small-cell lung cancer (NSCLC) with driver mutations, whether neoadjuvant immunotherapy could be clinically valuable in those patients warrants further investigation. We utilized 40 oncogene-mutant NSCLC treated with induction immunotherapy from a large consecutive multicenter cohort. Overall response rate was 62.

Preparation of Magnesium Hydroxide Flame Retardant from Hydromagnesite and Enhance the Flame Retardant Performance of EVA.

In this study, hydromagnesite, a rare natural hydrated alkaline magnesium carbonate, was used to synthesize magnesium hydroxide (MH) as a flame retardant for ethylene-vinyl acetate (EVA) to enhance its fire resistance and smoke suppression. Various concentrations of sodium hydroxide (NaOH) were used to alter the morphology and the flame-retardant efficiency of synthesized MH. EVA/MH composites were prepared through melt blending, and the influence of NaOH on the flame retardancy and mechanical properties was investigated by means of the limiting oxygen index (LOI), cone calorimeter test (CCT) and tensile test.

Real-World Scenario of Patients With Lung Cancer Amid the Coronavirus Disease 2019 Pandemic in the People's Republic of China.

Introduction: The coronavirus disease 2019 (COVID-19) outbreak throughout the world has affected millions of people in many ways, putting a huge burden on the health care system. The ongoing outbreak of this respiratory disease has posed critical challenges to public health, research, and medical communities around the world. This study aimed at evaluating the impact of COVID-19 pandemic on patients with lung cancer in the People's Republic of China.

Integrated Exploitation of the Structural Diversity Space of Chemotherapy Drugs to Selectively Inhibit HER2 T798M Mutant in Lung Cancer.

An acquired T798M gatekeeper mutation in human epidermal growth factor receptor 2 (HER2) kinase can cause drug resistance to anti-HER2 chemotherapy drugs in lung cancer. Previously, the reversible pan-kinase inhibitor staurosporine has been found to selectively inhibit the HER2 T798M mutant over wild-type kinase, suggesting that the staurosporine scaffold is potentially to develop mutant-selective inhibitors. Here, we systematically evaluated the chemical space of staurosporine scaffold-based compounds in response to HER2 T798M mutation at structural, energetic and molecular levels by using an integrated analysis strategy.

Promoter methylation of tumor suppressor genes in esophageal squamous cell carcinoma.

Esophageal squamous cell carcinoma (ESCC) is a prevalent and fatal cancer in China and other Asian countries. Epigenetic silencing of key tumor suppressor genes (TSGs) is critical to ESCC initiation and progression. Recently, many novel TSGs silenced by promoter methylation have been identified in ESCC, and these genes further serve as potential tumor markers for high-risk group stratification, early detection, and prognosis prediction.

E-cadherin promotes proliferation of human ovarian cancer cells in vitro via activating MEK/ERK pathway.

Aim: E-cadherin is unusually highly expressed in most ovarian cancers. This study was designed to investigate the roles of E-cadherin in the carcinogenesis and progression of ovarian cancers.

Methods: Human ovarian adenocarcinoma cell line SKOV-3 was examined.

Antagonistic effects of ultra-low-molecular-weight heparin on Aβ25-35-induced apoptosis in cultured rat cortical neurons.

Low-molecular-weight heparin (LMWH) and ultra-low-molecular-weight heparin (ULMWH) are heparin's derivatives, having various pharmacological effects. The present study aims to investigate the effect of ULMWH on amyloid β peptide (Aβ25-35)-induced neurotoxicity in cultured rat cortical neurons, and LMWH was employed as a positive control agent. The neurons were incubated with Aβ25-35 (35μM), Aβ25-35 plus ULMWH (2, 10, 50 μg/ml) or LMWH (10 μg/ml) for 24h.

TRIB3 functional Q84R polymorphism is a risk factor for metabolic syndrome and carotid atherosclerosis.

Objective: To determine the association of TRIB3 Q84R polymorphism with metabolic syndrome (MetS) and carotid atherosclerosis.

Research Design And Methods: A case-control study enrolled 513 Chinese subjects in three groups: control, MetS, and obese. The functional TRIB3 Q84R polymorphism was genotyped among subjects undergoing carotid ultrasonography.

Protective effect of ultra low molecular weight heparin on glutamate-induced apoptosis in cortical cells.

Purpose: To investigate the effect of ultra low molecular weight heparin (ULMWH) on glutamate induced apoptosis in rat cortical cells and to explore the possible mechanisms.

Materials And Methods: Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was first analyzed with Hoechst 33258 and then confirmed by DNA fragmentation.

Topology theory on rough sets.

For further studying the theories and applications of rough sets (RS), this paper proposes a new theory on RS, which mainly includes topological space, topological properties, homeomorphism, and its properties on RS by some new definitions and theorems given. The relationship between partition and countable open covering is discussed, and some applications based on the topological rough space and its topological properties are introduced. Moreover, some perspectives for future research are given.

Antagonistic effects of ultra-low-molecular-weight heparin against cerebral ischemia/reperfusion injury in rats.

Heparin and low-molecular-weight heparin have long been proposed for stroke treatment. This study was conducted to demonstrate the antagonistic effects of ultra-low-molecular-weight heparin (ULMWH) on cerebral ischemic injury in rats and the mechanisms underlying the effects. Male Wistar rats were subjected to middle cerebral artery occlusion (MCAO) for 2h followed by reperfusion for 24h.

The relationship between the structure of dermatan sulfate derivatives and their antithrombotic activities.

To study the relationship between the structure of dermatan sulfate (DS) derivatives and their anti-thrombotic activities, DS-derived oligosaccharides (with different structures and relative molecular weight (M(r))) were prepared, and the effects of the DS-derived oligosaccharides on the activities of heparin cofactor II (HCII), activated protein C (APC), blood platelet, and vascular endothelial cells were studied. The major disaccharides of DS and polysulfated dermatan sulfate (PSDS) were IdoA-1-->3-GalNAc-4-OSO(3) and IdoA-2OSO(3)-1-->3-GalNAc4, 6-diOSO(3), respectively. The results showed that the consequence of the thrombotic inhibitory effects of DS and its derivatives were as follows: PSDS>low molecular weight polysulfated dermatan sulfate (LPSDS)>DS.

[Effects of zinc on activity of NOS and expression of nNOS in hippocampus of acute hypoxic mice].

Aim: To explore the effect of zinc on vigor of nitric oxide synthase (NOS) and the expression level of neuronal nitric oxide synthase (nNOS) in the hippocampus of acute hypoxic mice and the protective effects of zinc.

Methods: Model of acute hypoxic mice was duplicated, NADPH-d histochemistry and nNOS immunohistochemistry were used to investigate the changes of NOS in different groups.

Results: Compared with the NS group, the hypoxia endurance of the zinc group was significantly increased; the number of NOS positive neurons and nNOS positive neurons in hippocampus and CA1 were significantly decreased.

[Protection of lithium on hippocampal cholecystokinin and nitric oxide synthase neuron in lead exposed rats].

Objective: To investigate the effect of lithium on hippocampal cholecystokinin (CCK) and neuronal nitric oxide synthase (nNOS) positive neurons and its relationship to the learning and memory ability of lead exposed rats.

Methods: Wistar rats were randomly divided into the control group, the lead group, four lead + LiCl (3, 30, 300, 3,000 mg/kg) groups. Four lead + LiCl groups were fed with food containing 3, 30, 300, 3,000 mg/kg LiCl respectively.

[Effects of taurine and zinc on activity of NOS and expression of nNOS in cerebral cortex of acute hypoxic mice].

Objective: To explore the effect of taurine and zinc on vigor of nitric oxide synthase (NOS) and the expression level of neuronal nitric oxide synthase (nNOS) in the cerebral cortex of acute hypoxic mice and the their neuroprotective effects.

Methods: Model of acute hypoxic mice was duplicated, NADPH-d histochemistry and nNOS immunohistochemistry were used to investigate the changes of NOS in different groups.

Results: Compared with the NS group, the anoxia endurance of the zinc sulfate group was increased 33.

[Effects of lithium on rat hippocampus cholecystokinin protein expression and learning and memory].

Objective: To investigate the effects of lithium on brain development, leaning and memory, we observed the effects of lithium chloride on rat body weight, learning ability and memory capacity.

Methods: Wistar rats were randomly divided into control group and four lithium chloride (LiCl) groups. Four LiCl groups were feed with food containing 3, 30, 300, 3000 mg/kg LiCl respectively.

[Inducible nitric oxide synthase gene expression in the testis of rats instilled with depleted uranium particles].

Objective: To explore genital toxicity of depleted uranium (DU) by studying the changes of inducible nitric oxide synthase (iNOS) in the testis of rats instilled with DU particles.

Methods: Wistar rats were exposed to DU by means of different dosages of DU particles intratracheal instillation. The samples of the testis were collected 3 months later, and iNOS mRNA was determined by reverse-transcription PCR (RT-PCR).

[Protection of zinc on hippocampal cholecystokinin of lead-exposed rat].

Objective: To investigate the relationship between the effects of zinc on hippocampal cholecystokinin (CCK) positive neurons and learning and memory ability of lead-exposed rats.

Methods: Thirty-six Wistar rats were divided into control group, lead-exposed group (drunk 6.15 mmol/L of lead solution) and lead-zinc group (drunk 6.

Inhibitory effect of heparin-derived oligosaccharides on secretion of interleukin-4 and interleukin-5 from human peripheral blood T lymphocytes.

Aim: To investigate the inhibitory effect of heparin-derived oligosaccharides (Oligs) on secretion of interleukin-4 (IL-4) and interleukin-5 (IL-5) from human peripheral blood T lymphocytes (PBTLs).

Methods: Oligs were prepared by three different heparin depolymerization methods and separated by gel filtration chromatography. PBTLs from ten adult patients with allergic eosinophilic gastroenteritis were treated with phytahematoagglutinin (PHA) and Oligs.

Effects of dermatan sulfate derivatives on platelet surface P-selectin expression and protein C activity in blood of inflammatory bowel disease patients.

Aim: To investigate the effect of dermatan sulfate (DS) derivatives on platelet surface P-selectin expression and blood activated protein C (APC) activity in patients with inflammatory bowel disease (IBD), and to clarity the anti-inflammatory mechanism of DS derivatives.

Methods: Dermatan sulfate (DS) was sulfated with chlorosulfonic acid to prepare polysulfated dermatan sulfate (PSDS). The major disaccharides of DS and PSDS were determined by 1H nuclear magnetic resonance spectroscopy (1H-NMR) and 13C-NMR.

[Effect of taurine on NOS activity in hippocampus of rat exposed lead].

Objective: To study the effect of taurine on lead-induced damage to the ability of learning and memory.

Methods: Using NADPH-d histochemistry method to study the change of rat NOS positive neurons in hippocampus. Rats in experimental groups were fed with different doses of lead in drinking water (0.