[Enhancing effect of matrine on the tumor-inhibition by TIM2 gene-modified hepatocarcinoma H22 cells in mice].

Objective: To investigate the effects of matrine on the anti-tumor efficiency of TIM2 gene-modified murine hepatocarcinoma H22 cells.

Methods: A combined eukaryotic expression vector pIRES2-EGFP-TIM2 was constructed and transfected into H22 cells by lipofectamin. The monoclone of positive H22-TIM2 cells and negative control H22-EGFP cells transfected with pIRES2-EGFP vector were selected by G418 pressure and limited dilution method in turn and were inoculated to establish the tumor-bearing mouse model.

[Effects of matrine on oncogenicity of H22 cells modified by TIM2 gene in vivo].

Objective: To investigate the effects of matrine on the anti-tumor efficiency of H22 murine hepatocarcinoma cell-based vaccine modified by TIM2 gene in vivo.

Method: The combinant eukaryotic expression vector pIRES2-EGFP-TIM2 was constructed and transfected into H22 cells by lipofectamin. The monoclone of the positive H22-TIM2 cells and negative control H22-EGFP cells were selected by G418 pressure and limited dilution method in turn.

[Inhibition of tumor growth in tumor-bearing mice treated with matrine].

Objective: To investigate the inhibitory effect of matrine on tumor growth in tumor-bearing mice and explore its possible mechanisms of anti-tumor action in vivo.

Methods: Hepatocellular carcinoma cells H(22) were subcutaneously injected into BALB/c mice and matrine was administered to the tumor-bearing mice. The kinetics of tumor formation and tumor growth were measured, tumor growth inhibition rate (IR) was calculated, and tumor tissue samples were taken and examined by light and electron microscopy to assess the inhibitory effects of matrine on tumor growth in the mice.

[Construction of eukaryotic expression vector of unknown KH gene and its effect on cell proliferation].

Objective: To construct an eukaryotic expression vector of open reading frame of unknown KH gene (KH-ORF), and investigate its effect on cell proliferation.

Methods: The pCI-neo-KH-ORF expression vector was constructed by DNA recombinant technique and was introduced into COS-7 cells and K562 cells by lipofectactin-mediated DNA transfection. Expression of KH-ORF mRNA was detected by RT-PCR.

[Gene expression profile in early differentiation of K562 cells induced by matrine].

Objective: To compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.

Methods: K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.

[Effects of matrine on the activity of protein tyrosine kinase and phosphatase in K562 cells].

Background & Objective: The differentiation of K562 cells can be induced by a given concentration of matrine. This study was designed to investigate the mechanism of signal transduction in induced differentiation of K562 cells.

Methods: ELISA coupled with streptavidin-biotin system was used to dynamically detect the activity of protein tyrosine kinase and phosphatase in the cytoplasm and the membrane of K562 cells treated by matrine.

[Matrine affects early expression of proto-oncogenes in K562 cells].

Background & Objective: It has been reported that matrine had the anti-tumor activity, and our previous study have provided that matrine had the effects of inhibiting proliferation and inducing differentiation in K562 cell line, but its molecular mechanism is unknown yet.

Method: The expression of several proto-oncogenes(c-myc, c-jun, H-ras, p21, and HNF-1 alpha) in K562 cell line treated by 0.2 mg/ml matrine were measured by RT-PCR.