csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis.

Background: Clonorchis sinensis (CS) is classified as a group 1 carcinogen and can cause intrahepatic cholangiocarcinoma (ICC). CS extracellular vesicles (CsEVs) play important roles in mediating communication between parasitic helminths and humans. Ferroptosis is a novel cell death mechanism that is mainly induced by lipid peroxidation and iron overload.

Gene expression profiling analysis of ovarian cancer.

As a gynecological oncology, ovarian cancer has high incidence and mortality. To study the mechanisms of ovarian cancer, the present study analyzed the GSE37582 microarray. GSE37582 was downloaded from Gene Expression Omnibus and included data from 74 ovarian cancer cases and 47 healthy controls.

[The binding property of different regions in Duffy-binding-like domain alpha of Plasmodium falciparum erythrocyte membrane protein 1 to heparin].

Objective: To clone and express Plasmodium falciparum erythrocyte membrane protein 1 DBLalpha (PfEMP1-DBLalpha) and three fragments genes, and screen the strongest affinity sequence with the red blood cell surface receptors-heparin or heparin sulfate in the structure of PfEMP1-DBLalpha.

Methods: The sequence of PfEMP1-DBLalpha1245 was optimized according to the characteristics of E. coli codon, synthesized, and divided into three fragments (DBLaA, DBLalphaB, and DBLalphaC) by PCR.

Analysis of var genes cloned from a Plasmodium falciparum isolate in China.

Objective: To analyse the var gene repertoire and characterise the chondroitin sulphate A (CSA)-binding activity of the Duffy-binding like (DBL) domains encoded by the var2csa gene of a Plasmodium falciparum (P. falciparum) isolate in Hainan Province, China.

Methods: The sequences of var DBL1 regions were PCR-amplified, sequenced and the sequence characteristics was bioinformatically analysed.

[Serological detection of Cryptosporidium spp. infection in outpatients in Changchun].

CP23 gene of Cryptosporidium parvum was expressed in Escherichia coli, and the recombinant protein was purified. Its immunoreactivity was analyzed by Western blotting. Serum samples were collected from outpatients of different ages from August to November, 2010 in Changchun.

[Cloning and expression of var2csa DBL domains from Plasmodium falciparum hainan isolate and functional analysis of the recombinant protein].

Objective: To clone and express three VAR2CSA duffy antigen-binding ligand (DBL) domains (DBL4/ 5/6) encoded by var2csa gene of a Hainan isolate of Plasmodium falciparum, and study the difference of chondroitin sulfate A (CSA)-binding activity among them.

Methods: Three DBL domains was amplified by PCR and cloned into the vector pMD18-T. The recombinant plasmids were identified by enzyme digestion and sequencing, and then subcloned into the prokaryotic expression vector pET-22b.

[Polysaccharide and molecular pathogenesis of Plasmodium falciparum].

In the interaction of Plasmodium falciparum with human cells, sporozoite adheres to the receptor of the liver endothelial cell, then invades to liver. Merozoite binds to the surface of red blood cells, and invades to erythrocyte. The adhesion of membrane protein of the infected erythrocytes to the surface molecules of vascular endothelial cell in the vital organs leads to the obstruction of blood circulation eventually.

[Screening for relevant proteins involved in adhesion of Cryptosporidium parvum sporozoites to host cells].

The Cryptosporidium parvum T7 phage display library was screened by using Caco-2 cells. Five specific gene fragments were identified by blasting sequences in GenBank, one of which encoding the CP2 protein was previously identified as a surface molecule of sporozoites and involved in parasite invasion. The others are hypothetic proteins with unknown functions.

[Research and perspectives in parasitology].

This article reviews the recent achievements in parasitology including new diagnostic techniques, molecular mechanism of parasitic pathogenesis, drug resistance, antigenic variation, parasite genomics and proteomics. The perspective development in the area is also discussed.

[Cloning and sequence analysis of a partial gene of Trichomonas vaginalis dsRNA virus].

A pair of degenerate primers were designed following the published nucleotide sequence of Trichomonas vaginalis virus(U08999, NC003873, NC003824, NC003834). Using the total nucleic acids extracted from the Trichomonas vaginalis as template, RT-PCR was performed with the primers to obtain a fragment of the TVV. The product was cloned, sequenced and compared with the sequences available in the GenBank.

[Construction and screening of recombinant fowlpox virus expressing Eimeria tenella F2 hybrid strain SO7 gene].

Objective: To construct the recombinant fowlpox virus expressing Eimeria tenella F2 hybrid strain SO7 gene.

Methods: A recombinant expression plasmid pUTA-SO7 was constructed by inserting the SO7 gene of Eimeria tenella F2 hybrid strain into downstream of a hybrid poxvirus promoter which was flanked by the TK gene of fowlpox virus (FPV). The constructed pUTA-SO7 was firstly transfected into chicken embryo fibroblast cells(CEF) pre-infected with FPV strain 282E4 by using liposome, then the viruses resulted from the transfection were selected for 2 passages by culturing in CEF cells with MEM medium containing 40 mg/L 5-bromo-2-deoxy-uridine (BrdU).

[Immunoprotection induced by recombinant plasmids pVAX1-Mzp5-7 of Eimeria tenella].

Objective: To observe the immunoprotection of recombinant plasmids of lambda Mzp5-7 to chicken challenged with Eimeria tenella (E. t) oocysts.

Methods: All the chickens were immunized with the recombinant plasmids by different inoculation pathways, three times on d7, d14 and d21, and challenged with E.

[Development of hybrid strains from geographic isolates of Eimeria tenella and their immunoprotection].

Objective: To cultivate hybrid strains from three geographic isolates of Eimeria tenella and to explore the possibility of developing vaccine candidates.

Methods: Three parental strains were selected from five geographic isolates of E. tenella through immune experiment, and hybrid strains were cultivated.

[Cloning and sequencing of the gp23 gene encoding a surface antigen on sporozoites of Cryptosporidium parvum].

Objective: To clone and sequence the gp23 gene encoding a surface antigen on the sporozoites of Cryptosporidium parvum.

Methods: Genomic DNA was isolated from oocysts of Cryptosporidium parvum. The gp23 gene was amplified by polymerase chain reaction (PCR) and cloned into pMD18-T vector and sequenced by the methods of dideoxy-mediated chain termination.

[Cloning of a species-specific gene fragment from Cryptosporidium parvum and the development of diagnostic PCR primers].

Objective: To develop a pair of diagnostic PCR primers for Cryptosporidium parvum.

Methods: A species-specific gene fragment of C. parvum was obtained through RAPD analysis.