Study on the impact of children's myopia on parental anxiety levels and its related factors.

Objective: To assess how children's myopia affects the psychological state of their parents, especially their anxiety levels, and its related factors.

Methods: Using a cross-sectional case series design, this study employed the Beck Anxiety Inventory (BAI) to assess the anxiety status of parents of myopic children who visited Beijing Children's Hospital from May to August 2024. Data were statistically analyzed using Chi-square test, t-test, and analysis of variance, and Spearman correlation analysis was used to explore the relationship between myopia and anxiety.

Immune modulation of Th1/Th2/Treg/Th17/Th9/Th21 cells in rabbits infected with .

Introduction: Despite long-term integrated control programs for Eimeria stiedai infection in China, hepatic coccidiosis in rabbits persists. Th1, Th2, Th17, Treg, Th9, and Th21 cells are involved in immune responses during pathogen infection. It is unclear whether Th cell subsets are also involved in E.

Novel lncRNA-prader willi/angelman region RNA, SNRPN neighbour (PWARSN) aggravates tubular epithelial cell pyroptosis by regulating TXNIP via dual way in diabetic kidney disease.

Objectives: Elevated thioredoxin-interacting protein (TXNIP)-induced pyroptosis contributes to the pathology of diabetic kidney disease (DKD). However, the molecular mechanisms in dysregulated TXNIP in DKD remain largely unclear.

Materials And Methods: Transcriptomic analysis identified a novel long noncoding RNA-Prader Willi/Angelman region RNA, SNRPN neighbour (PWARSN)-which was highly expressed in a proximal tubular epithelial cell (PTEC) under high glucose conditions.

Two-dimensional shear wave elastography with two different systems for the diagnosis of breast lesions.

Background: Two-dimensional (2D) - shear wave elastography (SWE) has made promising advances in the diagnostic of breast lesions. However, few studies have assessed whether the diagnostic effectiveness of different platforms employing 2D-SWE is equal or different.

Objective: To compare the diagnostic effectiveness of 2D-SWE techniques from two different systems in differentiating malignant breast lesions from benign ones.

Chemically modified phosphorene as efficient catalyst for hydrogen evolution reaction.

Hydrogen gas produced by electrolysis has been considered as an excellent alternative to fossil fuels. Developing non-noble metal catalysts with high electrocatalytic activities is an effective way to reduce the cost of hydrogen production. Recently, black phosphorus (BP) based materials have been reported to have good potential as electrocatalysts for hydrogen evolution reaction (HER).

[Glaucoma and Moscow Eye Microsurgery complex (Moroz) keratoprosthesis].

Objective: To investigate the relationship between glaucoma and Moscow Eye Microsurgery Complex (Moroz) keratoprosthesis.

Methods: Analysis and retrospective review of consecutive clinical case series. A total of 90 patients had a Moroz keratoprosthesis implantation between Apr, 2000 and Jun, 2011 at our hospital.

Ocular trauma score in transferred fireworks-related ocular injuries: a case series.

Background: Our aim was to review the characteristics of transferred fireworks-related ocular damage and to evaluate the prognostic value of the ocular trauma score (OTS) for these injuries.

Methods: This study included 22 transferred patients (19 male, 3 female; mean age 22.6±14.

Patients' subjective visual experiences during vitreous surgery under local anesthesia.

Objective: To investigate patient's visual experience during vitreous surgery under local anesthesia.

Methods: A prospective and randomized study of 76 patients that underwent vitreous surgeries in our hospital between July 2010 and December 2010 was designed. All patients were interviewed half an hour before and within 1 hour after the surgery using a standardized questionnaire.

[The observation of the effects of a micromolecular compound J2 on rat corneal allograft using atomic force microscopy].

Objective: To study the cell membrane of corneal endothelium with a micromolecular compound J2 in corneal allograft of rat using atomic force microscopy (AFM).

Methods: Cohort study. Subjects were divided into two groups: group A (n = 15): experimental group; group B (n = 15): placebo control group.

[Construction of lentiviral expression vector containing Foxp3 gene and its expression in DC2.4 cell line].

Aim: To construct a Foxp3 lentiviral vector and transfer it into DC2.4 cells, which provides Foxp3+DC cells for further study on its immune modulation.

Methods: We cloned mouse Foxp3 gene into lentiviral vector(pGC-FU) and acquired the plasmid pGC-FU-Foxp3.

Experimental study of the mechanism of tolerance induction in dexamethasone-treated dendritic cells.

Background: The aim of this study was to investigate the mechanisms underlying tolerance induction of dexamethasone (Dex)-treated dendritic cells (DCs).

Material/methods: Well-grown DC2.4 cells were randomly assigned to receive control, 50 µg/L, 100 µg/L, or 200 µg/L of dexamethasone and then were cultured for 6 days.

Two-staged nuclear transfer can enhance the developmental ability of goat-sheep interspecies nuclear transfer embryos in vitro.

The technique of interspecies somatic cell nuclear transfer, in which interspecies cloned embryos can be reconstructed by using domestic animal oocytes as nuclear recipients and endangered animal or human somatic cells as nuclear donors, can afford more opportunities in endangered animal rescue and human tissue transplantation, but the application of this technique is limited by extremely low efficiency which may be attributed to donor nucleus not fully reprogrammed by xenogenic cytoplasm. In this study, goat fetal fibroblasts (GFFs) were used as nuclear donors, in vitro-matured sheep oocytes were used as nuclear recipients, and a two-stage nuclear transfer procedure was performed to improve the developmental ability of goat-sheep interspecies clone embryos. In the first stage nuclear transfer (FSNT), GFFs were injected into the ooplasm of enucleated sheep metaphase-II oocytes, then non-activated reconstructed embryos were cultured in vitro, so that the donor nucleus could be exposed to the ooplasm for a period of time.

[The progress of corneal crosslink in corneal disease].

Corneal crosslink through UVA-riboflavin photochemistry has been shown to be an effective treatment for keratoconus, related keratectasias and infectious melting keratitis that did not respond to systemic therapy. It is a new approach to increase the mechanical and biochemical stability of the stromal tissue. The purpose of this paper is to review the basic principle, procedure, laboratory and clinical researches about corneal crosslink.

New surgical therapy for conjunctival lymphangiectasia.

Aim: To report 7 cases of conjunctival lymphangiectasia, introduce a simple operation method and discuss the result of the pathological examination.

Methods: Clinical data including medical history, physical examination, previous surgery history were collected in 7 patients with conjunctival lymphangiectasia. Ocular data including visual acuity, Slit-lamp biomicroscopy were released, as were results of histopathology studies.

Estrogen regulates expression of osteoprotegerin and RANKL in human periodontal ligament cells through estrogen receptor beta.

Background: The function of periodontal ligament (PDL) cells may be affected by estrogen. PDL cells synthesize the receptor activator of nuclear factor-kappa B ligand (RANKL) and its decoy receptor, namely, osteoprotegerin (OPG), which directly controls osteoclastogenesis. The primary aim of this study was to investigate how estrogen affects the expression of OPG and RANKL in human PDL (hPDL) cells.

Sequential modulation of growth factors: a novel strategy for adoptive immunotherapy of acute myeloid leukemia.

Evidence from allogeneic hematopoietic stem cell transplantation indicates a possible immune response against leukemia-associated antigens in patients with either acute myeloid leukemia (AML) or chronic myeloid leukemia (CML). However, autologous immune responses are less evident. We have developed a method using sequential modulation of growth factors (SMGF) to generate specific anti-AML T-cells from primary cultures of mononuclear cells (MNCs) from patients with AML.