[Analysis of sensitization effect of chimeric allergen TAT-IhC-R8 derived from major allergen group 1 genes of dust mites].

Objective: To explore the sensitization effect of allergen TAT-IhC-R8, derived from major allergen group 1 genes of dust mites.

Methods: Forty BALB/c mice were randomly divided into 4 groups, namely PBS group, ovalbumin (OVA) group, R8 group and TAT-IhC-R8 (TIR8) group, 10 mice each group. All the mice in OVA, R8 and TIR8 groups were treated with corresponding allergens (10 µg/ml) on the 0, 7th and 14th day by intraperitoneal injection and nebulized inhalation on day 21 with the concentration of 30 min/d for 7 days.

[Effect of immunotherapy with recombinant allergen group 3 from dermatophagoides farinae in asthma mice].

Objective: To explore the effect of specific immunotherapy with major 3 group recombinant allergen rDer f 3 of Dermatophagoides farinae in murine asthma model.

Methods: Forty BALB/c mice were randomly divided into 4 groups, namely PBS group (negative control), ovalbumin(0VA) group (positive control), rDerf3 allergen sensitization group (asthma group), and rDerf3 specific immunotherapy group(SIT group). The mice in asthma group and SIT group were injected intraperitoneally with purified rDer f 3 protein on days 0, 7 and 14, respectively, and rDer f 3 solution was inhalated from day 21 for 7 days.

[Prediction and identification of T-cell epitopes in major group 3 allergen derived from Dermatophagoides farina].

Objective: To predict and identify T cell epitopes of major group 3 allergen derived from Dermatophagoidesfarina (Der f 3).

Methods: The T cell epitopes of Der f 3 were analyzed through the sequence analysis by using the bioinformatics online tools. The five predicted peptides of T-cell epitopes were artificially synthesized.

[Prediction and identification of linear B-cell epitopes in major group 3 allergen derived from Dermatophagoides farina].

Objective: To predict and identify the linear B-cell epitopes in the major group 3 allergen derived from Dermatophagoides farina (Der f 3).

Methods: The linear B-cell epitopes of Der f 3 allergen were analyzed based on the physicochemical properties of amino acids including antigenicity, surface accessibility, flexibility, hydrophilicity, beta-turn by online bioinformatics softwares. The eight predicted peptides of linear B-cell epitopes were artificially synthesized and incubated with three aller gic serum pools (4 serum samples in each), which were consisted of total 12 serum samples from the allergic individuals, and the strong positive epitopes were selected.

Generation of a chimeric dust mite hypoallergen using DNA shuffling for application in allergen-specific immunotherapy.

Specific immunotherapy (SIT) is the only treatment that provides long lasting relief of allergy symptoms. Unfortunately, SIT-based traditional remedies carry the risk of producing local and/or systemic side effects. To improve the safety and efficacy of SIT, it has been proposed that SIT must utilize allergens that are hypoallergenic but hyperimmunogenic.

Acaroid mite allergens from the filters of air-conditioning system in China.

Accumulation of acaroid mites in the filters of air-conditioners is harmful to human health. It is important to clarify the allergen components of mites from the filters of local air-conditioning system. The present study was to detect the allergen types in the filters of air-conditioners and assesse their allergenicity by asthmatic models.